全文获取类型
收费全文 | 351篇 |
免费 | 14篇 |
专业分类
365篇 |
出版年
2022年 | 1篇 |
2021年 | 3篇 |
2020年 | 4篇 |
2019年 | 1篇 |
2018年 | 6篇 |
2017年 | 4篇 |
2016年 | 2篇 |
2015年 | 8篇 |
2014年 | 12篇 |
2013年 | 25篇 |
2012年 | 17篇 |
2011年 | 17篇 |
2010年 | 8篇 |
2009年 | 15篇 |
2008年 | 21篇 |
2007年 | 18篇 |
2006年 | 17篇 |
2005年 | 17篇 |
2004年 | 14篇 |
2003年 | 21篇 |
2002年 | 16篇 |
2001年 | 9篇 |
2000年 | 10篇 |
1999年 | 9篇 |
1998年 | 3篇 |
1997年 | 6篇 |
1996年 | 10篇 |
1995年 | 11篇 |
1994年 | 10篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 6篇 |
1989年 | 4篇 |
1988年 | 3篇 |
1987年 | 3篇 |
1986年 | 3篇 |
1985年 | 4篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1980年 | 2篇 |
1979年 | 4篇 |
1978年 | 2篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1974年 | 1篇 |
1973年 | 4篇 |
1970年 | 2篇 |
1968年 | 1篇 |
排序方式: 共有365条查询结果,搜索用时 15 毫秒
91.
Two striking fungi, isolated from soils from the island of Hachijo-jima in Japan, are described and illustrated. One of these
represents a new species ofTalaromyces (Ascomycetes), for which the nameTalaromyces hachijoensis is proposed. The new species is distinctive among all other recognized species in its restricted growth on common media,
yellow ascomata characterized by asci developing in chains, striate ascospores, and the absence of an anamorph. The other
isHeterocephalum taiense, a rare hyphomycete which has only previously been recorded from lvory Coast soil and probably from Panama soil. 相似文献
92.
Sekine S Shichiri M Bernier S Chênevert R Lapointe J Yokoyama S 《Structure (London, England : 1993)》2006,14(12):1791-1799
Glutamyl-tRNA synthetase (GluRS) is one of the aminoacyl-tRNA synthetases that require the cognate tRNA for specific amino acid recognition and activation. We analyzed the role of tRNA in amino acid recognition by crystallography. In the GluRS*tRNA(Glu)*Glu structure, GluRS and tRNA(Glu) collaborate to form a highly complementary L-glutamate-binding site. This collaborative site is functional, as it is formed in the same manner in pretransition-state mimic, GluRS*tRNA(Glu)*ATP*Eol (a glutamate analog), and posttransition-state mimic, GluRS*tRNA(Glu)*ESA (a glutamyl-adenylate analog) structures. In contrast, in the GluRS*Glu structure, only GluRS forms the amino acid-binding site, which is defective and accounts for the binding of incorrect amino acids, such as D-glutamate and L-glutamine. Therefore, tRNA(Glu) is essential for formation of the completely functional binding site for L-glutamate. These structures, together with our previously described structures, reveal that tRNA plays a crucial role in accurate positioning of both L-glutamate and ATP, thus driving the amino acid activation. 相似文献
93.
Two onygenalean fungi isolated from forest soil in the Sikhote-Alin reserve, Russian Far East (east Siberia), are described
and illustrated:Gymnostellatospora parvula as a new species andAphanoascus canadensis as a new record.Gymnostellatospora parvula is characterized by psychrophilic growth, pale yellow to pale cinnamon ascomata with a hyphal peridium, small, hyaline discoid
ascospores with an equatorial rim and more or less longitudinally ridged wall. 相似文献
94.
95.
Shimazaki M Miyamoto Y Yamamoto K Yamada S Takami M Shinki T Udagawa N Shimizu M 《Bioorganic & medicinal chemistry》2006,14(13):4645-4656
96.
97.
Development and evaluation of an automated workstation for single nucleotide polymorphism discrimination using bacterial magnetic particles 总被引:4,自引:0,他引:4
Tanaka T Maruyama K Yoda K Nemoto E Udagawa Y Nakayama H Takeyama H Matsunaga T 《Biosensors & bioelectronics》2003,19(4):325-330
We designed an automated workstation for magnetic particle-based single nucleotide polymorphism (SNP) discrimination of ALDH genotypes. Bacterial magnetic particles (BMPs) extracted from Magnetospirillum magneticum AMB-1 were used as DNA carriers. The principle for SNP discrimination in this study was based on fluorescence resonance energy transfer (FRET) between FITC (donor) and POPO-3 (acceptor) bound to double-stranded DNA. The workstation is equipped with a 96-way automated pipetter which collects and dispenses fluids as it moves in x- and z-directions. The platform contains a disposable tip rack station, a reagent vessel serving as a stock for POPO-3 and FITC-labeled probes and a reaction station for a 96-well microtiter plate. BMPs were collected by attaching a neodymium iron boron sintered (Nd-Fe-B) magnet on the bottom of the microtiter plate. This system permits the simultaneous heating and magnetic separation of 96 samples per assay. The genotypes ALDH2*1 and ALDH2*2 were discriminated by calculating the relative fluorescence intensities on BMPs. 相似文献
98.
Oka M Okada T Nakamura S Ohba M Kuroki T Kikkawa U Nagai H Ichihashi M Nishigori C 《FEBS letters》2003,554(1-2):179-183
The regulation of phospholipase D1 (PLD1) by protein kinase C (PKC) isoforms was analyzed in human melanoma cell lines. 12-O-Tetradecanoylphorbol-13-acetate (TPA)-induced PLD1 activation was suppressed by the introduction of PKCdelta as well as its kinase-negative mutant in MeWo cells, which contain PKCalpha but lack PKCbeta. PLD activity was not affected by PKCdelta in G361 cells, which have PKCbeta but are deficient in PKCalpha. In MeWo cells introduced by PKCalpha and PLD1, the association of these proteins was observed, which was enhanced by the TPA treatment. In cells overexpressing PKCdelta in addition to PKCalpha and PLD1, TPA treatment increased the association of PKCdelta and PLD1, while it attenuated the association of PKCalpha and PLD1. These results indicate that PKCdelta inhibits TPA-induced PLD1 activation mediated by PKCalpha through the association with PLD1. 相似文献
99.
Strategic compartmentalization of Toll-like receptor 4 in the mouse gut 总被引:23,自引:0,他引:23
Ortega-Cava CF Ishihara S Rumi MA Kawashima K Ishimura N Kazumori H Udagawa J Kadowaki Y Kinoshita Y 《Journal of immunology (Baltimore, Md. : 1950)》2003,170(8):3977-3985
Pattern recognition receptors (PRRs), which include the Toll-like receptors (TLRs), are involved in the innate immune response to infection. TLR4 is a model for the TLR family and is the main LPS receptor. We wanted to determine the expression of TLR4 and compare it with that of TLR2 and CD14 along the gastrointestinal mucosa of normal and colitic BALB/c mice. Colitis was induced with 2.5% dextran sodium sulfate (DSS). Mucosa from seven segments of the digestive tract (stomach, small intestine in three parts, and colon in three parts) was isolated by two different methods. Mucosal TLR4, CD14, TLR2, MyD88, and IL-1beta mRNA were semiquantified by Northern blotting. TLR4 protein was determined by Western blotting. TLR4/MD-2 complex and CD14 were evaluated by immunohistochemistry. PRR genes were constitutively expressed and were especially stronger in colon. TLR4 and CD14 mRNA were increased in the distal colon, but TLR2 mRNA was expressed more strongly in the proximal colon, and MyD88 had a uniform expression throughout the gut. Accordingly, TLR4 and CD14 protein levels were higher in the distal colon. TLR4/MD-2 and CD14 were localized at crypt bottom epithelial cells. TLR4/MD2, but not CD14, was found in mucosal mononuclear cells. Finally, DSS-induced inflammation was localized in the distal colon. All genes studied were up-regulated during DSS-induced inflammation, but the normal colon-stressed gut distribution was preserved. Our findings demonstrate that TLR4, CD14, and TLR2 are expressed in a compartmentalized manner in the mouse gut and provide novel information about the in vivo localization of PRRs. 相似文献
100.
Nakahara H Nishimura S Inoue M Hori G Amari S 《Bioinformatics (Oxford, England)》2003,19(9):1124-1131
MOTIVATION: Given the vast amount of gene expression data, it is essential to develop a simple and reliable method of investigating the fine structure of gene interaction. We show how an information geometric measure achieves this. RESULTS: We introduce an information geometric measure of binary random vectors and show how this measure reveals the fine structure of gene interaction. In particular, we propose an iterative procedure by using this measure (called IPIG). The procedure finds higher-order dependencies which may underlie the interaction between two genes of interest. To demonstrate the method, we investigate the interaction between the two genes of interest in the data from human acute lymphoblastic leukemia cells. The method successfully discovered biologically known findings and also selected other genes as hidden causes that constitute the interaction. AVAILABILITY: Softwares are currently not available but are possibly made available in future at http://www.mns.brain.riken.go.jp/~nakahara/DNA_pub.html where all the related information is also linked. 相似文献