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141.
Shun-ichi Wada Anna Takesada Yurie Nagamura Eri Sogabe Rieko Ohki Junsuke Hayashi Hidehito Urata 《Bioorganic & medicinal chemistry letters》2017,27(24):5378-5381
The conjugation of Aib-containing amphipathic helical peptide with cyclo(-Arg-Gly-Asp-d-Phe-Cys-) (cRGDfC) at the C-terminus of the helix peptide (PI) has been reported to be useful for constructing a carrier for targeted siRNA delivery into cells. In order to explore structure–activity relationships for the development of potential carriers for siRNA delivery, we synthesized conjugates of Aib-containing amphipathic helical peptide with cRGDfC at the N-terminus (PII) and both the N- and C-termini (PIII) of the helical peptide. Furthermore, to examine the influence of PI helical chain length on siRNA delivery, truncated peptides containing 16 (PIV), 12 (PV), and 8 (PVI) amino acid residues at the N-terminus of the helical chain were synthesized. PII and PIII, as well as PI, could deliver anti-luciferase siRNA into cells to induce the knockdown of luciferase stably expressed in cells. In contrast, all of the truncated peptides were unlikely to transport siRNA into cells. 相似文献
142.
A new genus, Neocarpenteles, the Trichocomaceae (Eurotiales), and a new combination, N. acanthosporum, are proposed to accommodate Hemicarpenteles acanthosporus Udagawa et Takada. The fungus is characterized by sclerotioid, nonostiolate, unilocular stromata in which asci gradually
produce, outward from the center, lenticular ascospores with two equatorial crests and convex surfaces ornamented by triangular
ridges and microtubercles, and an Aspergillus anamorph with uniseriate aspergilla. It has the Q-10 system as the major ubiquinone.
Received: May 1, 2001 / Accepted: July 4, 2001 相似文献
143.
Cordyceps owariensis f. viridescens forma nov. (Nom. Jap., Amami-yarinohosemitake) is described from an underground nymph of Platypleura kuroiwae collected in Amami-oshima Island, southwestern Japan. Cultural isolations were made from ascospores of the fresh material
of this fungus, and consequently a new hyphomycete was developed as its anamorph. Nomuraea owariensis is described to accommodate this anamorphic state. This is the first report on the association of a Nomuraea anamorph with cicadicolous Cordyceps species.
Received: June 6, 2001 / Accepted: December 18, 2001 相似文献
144.
Shun-ichi Wada Shigeki Matsunaga Nobuhiro Fusetani Shugo Watabe 《Marine biotechnology (New York, N.Y.)》1999,1(4):337-341
The effect of a marine bicyclic peptide, theonellamide F, on vacuole formation in exponentially growing 3Y1 rat embryonic
fibroblasts was studied in comparison with the effect of monensin. Many abnormally large vacuoles appeared around the nucleus
in the cells treated with 6 μM theonellamide F within 24 hours. Following prolonged treatment with this agent, the number
of enlarged vacuoles increased. Such vacuoles accumulated many granules that showed Brownian movement. The effect of theonellamide
F on the cells was more drastic in an amino-acid-deficient medium, in which all cells died within 1 hour at a 3-μM concentration
of the agent. Theonellamide F probably affects cellular autophagy, inhibiting the degradation of the organelles and turnover
of proteins. Monensin, a well-known Na+ ionophore that disintegrates the Golgi apparatus, induced similar types of vacuole formation, although the vacuoles were
localized in a region slightly distant from the nucleus. Monensin readily affected cell morphology, resulting in cell death.
We propose that theonellamide F, like monensin, is a useful agent for investigating membrane structures in cells.
Received November 25, 1998; accepted February 10, 1999 相似文献
145.
Morimoto R Uehara S Yatsushiro S Juge N Hua Z Senoh S Echigo N Hayashi M Mizoguchi T Ninomiya T Udagawa N Omote H Yamamoto A Edwards RH Moriyama Y 《The EMBO journal》2006,25(18):4175-4186
Osteoclasts are involved in the catabolism of the bone matrix and eliminate the resulting degradation products through transcytosis, but the molecular mechanism and regulation of transcytosis remain poorly understood. Upon differentiation, osteoclasts express vesicular glutamate transporter 1 (VGLUT1), which is essential for vesicular storage and subsequent exocytosis of glutamate in neurons. VGLUT1 is localized in transcytotic vesicles and accumulates L-glutamate. Osteoclasts secrete L-glutamate and the bone degradation products upon stimulation with KCl or ATP in a Ca2+-dependent manner. KCl- and ATP-dependent secretion of L-glutamate was absent in osteoclasts prepared from VGLUT1-/- knockout mice. Osteoclasts express mGluR8, a class III metabotropic glutamate receptor. Its stimulation by a specific agonist inhibits secretion of L-glutamate and bone degradation products, whereas its suppression by a specific antagonist stimulates bone resorption. Finally, it was found that VGLUT1-/- mice develop osteoporosis. Thus, in bone-resorbing osteoclasts, L-glutamate and bone degradation products are secreted through transcytosis and the released L-glutamate is involved in autoregulation of transcytosis. Glutamate signaling may play an important role in the bone homeostasis. 相似文献
146.
Shuji Udagawa Satoshi Sakami Takahiro Takemura Mikiya Sato Takahiro Arai Aiko Nitta Takumi Aoki Koji Kawai Tomokatsu Iwamura Seiji Okazaki Takehiro Takahashi Mie Kaino 《Bioorganic & medicinal chemistry letters》2013,23(6):1617-1621
A series of novel 5-trans-hydroxyadamantan-2-yl-5,6,7,8-tetrahydropyrazolo[4,3-c]azepin-4(1H)-ones that inhibit 11beta-hydroxysteroid dehydrogenase type 1 are described. We discovered these 7-membered cyclic amide derivatives by introducing a distinctive linker through pharmacophore analysis of known ligands included in X-ray co-crystal structures. Further optimization using docking studies led to highly potent inhibitors 15b and 27, which furthermore showed the potent efficacy in in vivo studies. 相似文献
147.
148.
Cold adaptation of the terrestrial isopod,Porcellio scaber,to subnivean environments 总被引:1,自引:1,他引:0
K. Tanaka T. Udagawa 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1993,163(6):439-444
The terrestrial isopod, Porcellio scaber, was susceptible to subzero temperature: both freezing and chilling were injurious. The level of cold hardiness against chilling and freezing showed different patterns in their seasonal variation. The lower lethal temperature causing 50% mortality, an indicator of the tolerance to chilling, ranged from-1.37°C in August to-4.58°C in December. The whole body supercooling point, the absolute limit of freeze avoidance, was kept at about-7°C throughout the year. The winter decrease in lower lethal temperature was concomitant with an accumulation of low molecular weight carbohydrates which are possible protective reagents against chilling injury, whereas the less seasonally variable supercooling point seemed to be associated with the year-round presence of gut content. Food derivatives may act as efficient ice nucleators. The different trend in seasonal changes between lower lethal temperature and supercooling point may be related to the microclimate of the hibernacula in subnivean environments, where the winter temperature became lower than the lower lethal temperature in the summer active phase, but remained higher than the summer supercooling point.Abbreviations LLT50
lower lethal temperature inducing 50% mortality
- SCP
supercooling point
-
T
a
ambient air temperature
-
T
s
soil surface temperature 相似文献
149.
Sakae Tanaka Naoyuki Takahashi Nobuyuki Udagawa Hiroshi Murakami Ichiro Nakamura Takahide Kurokawa Tatsuo Suda 《Journal of cellular biochemistry》1995,58(4):424-435
Involvement of tyrosine phosphorylation in osteoclastic bone resorption was examined using osteoclast-like multinucleated cells prepared from co-cultures of mouse osteoblastic cells and bone marrow cells in the presence of 1α,25-dihydroxyvitamin D3. When osteoclast-like cells were plated on culture dishes in the presence of 10% fetal bovine serum, they were sharply stained in their peripheral region by anti-phosphotyrosine antibody. Western blot analysis revealed that 115-to 130-kD proteins were tyrosine-phosphorylated in osteoclast-like cells. Using immunoprecipitation and immunoblotting, one of the proteins with 115–130 kD was identified as focal adhesion kinase (p125FAK), a tyrosine kinase, which is localized in focal adhesions. Immunostaining with anti-p 125FAK antibody revealed that p125FAK was mainly localized at the periphery of osteoclast-like cells. Herbimycin A, a tyrosine kinase inhibitor, not only suppressed tyrosine phosphorylation of p125FAK but also changed the intracellular localization of p125FAK and disrupted a ringed structure of F-actin-containing podosomes in osteoclast-like cells. Antisense oligodeoxynucleotides to p125FAK inhibited dentine resorption by osteoclast-like cells, whereas sense oligodeoxynucleotides did not. These results suggest that p125FAK is involved in osteoclastic bone resorption and that tyrosine phosphorylation of p125FAK is critical for regulating osteoclast function. 相似文献
150.
Isolation of ascomycetous microfungi from 58 house dust samples from detached and apartment dwellings around Kobe City revealed
that species ofTalaromyces, Eurotium andNeosartorya were common ascomycetous propagules in the house dust.Neosartorya species accounted for 8.3% of the total identified isolates, of whichN. pseudofischeri was the main constituent, indicating that it may be a common potential fungal pathogen in the dwelling environment. Based
on the specimens collected,N. pseudofischeri is described and illustrated as new to Japan. 相似文献