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排序方式: 共有1219条查询结果,搜索用时 31 毫秒
921.
Kazuo Matsumoto Shuji Uchida Kunio Takeyasu Hiroshi Higuchi Hiroshi Yoshida 《Life sciences》1982,31(3):211-220
Trypsin-treatment of the microsome fraction of the ileum and the synaptic membrane fraction of the cerebral cortex of guinea-pig caused selective reduction in the apparent affinity of an agonist (carbachol), but not an antagonist (atropine), to muscarinic acetylcholine receptors (mAChR), measured as inhibition of binding of 3H-quinuclidinyl benzilate (3H-QNB). This effect was similar to that of Gpp(NH)p. The effects of trypsin and Gpp(NH)p were not additive. On the other hand, treatment of these fractions with 5,5'-dithiobis (2-nitrobenzoic acid) (DTNB) increased the apparent affinity of agonist, but not antagonist. The effect of DTNB predominated over those of trypsin and Gpp(NH)p, when the fractions were treated with two reagents simultaneously. 相似文献
922.
Kozo Kaibuchi Yoshimi Takai Yasuhiro Ogawa Shuji Kimura Yasutomi Nishizuka Toshikazu Nakamura Akito Tomomura Akira Ichihara 《Biochemical and biophysical research communications》1982,104(1):105-112
There appear to be considerable differences among tissues in the inhibitory action of adenosine 3′,5′-monophosphate (cyclic AMP) on phosphatidylinositol (PI) turnover induced by various extracellular signals. The present studies were on human peripheral lymphocytes and rat hepatocytes. In the lymphocyte system, cells are activated by phytohemagglutinin that induces PI turnover, and this PI turnover and cellular activation are profoundly blocked by dibutyryl cyclic AMP as well as by prostaglandin E1 which markedly increases cyclic AMP. In contrast, in the hepatocyte system, glycogenolysis is enhanced by α-agonists that induce PI turnover as well as by β-agonists and glucagon that increase cyclic AMP. In these cells the two classes of receptors appear to function independently, and PI turnover is not inhibited by cyclic AMP. 相似文献
923.
Summary Transport of benzenesulfonic acid derivatives through the rat erythrocyte membrane was studied. The transport properties, such as pH-dependence and effects of reagents reacting with amino-groups, were similar to those of anions like Cl– through the human erythrocyte membrane. The rate of transport of anions through rat erythrocyte membranes is higher than through those of other mammals, such as guinea pig and bovine erythrocyte membranes. This relatively high rate of transport makes the rat erythrocyte membrane suitable for use in comparative studies on the transports of slowly penetrating substances, such as organic anions. The transport velocities of benzenesulfonic acid derivatives were compared with their physico-chemical properties. It was shown that the hydrophobicity has no effect on the transport, but the electronic property has a significant effect: the transport rate is mainly dependent on thee
– donor capacities. This feature is the inverse to the well-known inhibitory effect of these derivatives on other anion transport: the inhibition is mainly dependent on thee
– acceptor capacities. It is suggested that the transport is regulated by the binding capacity of anions to the transport site. 相似文献
924.
Naohiro Kayama Kimiko Sakaguchi Shuji Kaneko Tetsuhiro Kubota Taira Fukuzawa Sumio Kawamura Tanihiro Yohimoto Shozo Yamamoto 《Prostaglandins & other lipid mediators》1981,21(4):543-554
1-Alkylimidazole derivatives of various sidechain lengths with various functional groups at the terminal end of the alkychain inhibited the synthesis of thromboxane A2 from arachidonic acid by rabbit platelets and the conversion of prostaglandin H2 to thromboxane A2 by the microsomes of rabbit platelets. These enzyme inhinitors were anti-aggregatory as examine with rabbit and human platelet-rich plasma under various experimental conditions. 相似文献
925.
926.
Fujitani Noboru Kawaguchi Naomasa Toda Shuji Matsumura Sueo Kimura Hiroshi Onishi Shunzo 《Molecular and cellular biochemistry》1997,169(1-2):73-78
An immunocytochemical study was performed to examine the expression of cellular c-myc protein in the heart of 30-, 120- and 180-day-old cardiomyopathic Syrian UM-X7.1 hamsters. The heart of age- and sex-matched BIO-RB hamster was used as normal control. In paraffin sections, an immunostaining for c-myc was markedly increased in cytoplasm of cells from the UM-X7.1 heart as compared with that of the BIO-RB heart which showed a weak staining. However, c-myc was localized in nuclei of cells in frozen sections of the heart. Specific cell types of the heart were differentiated with anti-vimentin, and we found that the increased expression of c-myc was present in nuclei of muscle cells of the UM-X7.1 myocardium. A quantitative study of c-myc-positive nuclei of muscle and nonmuscle cells was carried out by a video micrometer. The mean number of c-myc-positive nuclei of muscle cells was significantly higher in the cardiomyopathic heart than in the control heart from hamsters of all ages studied. These results suggest that the increase of c-myc protein may relate to the pathological state or pathogenesis of the hereditary cardiomyopathy. 相似文献
927.
Makoto Nagai Hisashi Yamada Shuji Nakada Keiko Ochi Tadashi Nemoto Shinobu Takahara Sadayori Hoshina Junko Horiguchi-Yamada 《Molecular and cellular biochemistry》1995,144(2):191-195
The antiproliferative effect of roxithromycin (RXM) was studied using human myeloid leukemia HL60 cells. RXM inhibited the growth of HL60 cells in a concentration-dependent manner, and significantly inhibited growth at concentrations above 75 M. This growth inhibition was not associated with specific cell cycle arrest and DNA synthesis was not impaired. In addition, the number of viable cells remained almost unchanged in the presence of 100 M RXM. RXM induced growth inhibition at least partly by the formation of multinucleate cells. Both flowcytometric and morphological examination revealed that more than 40% of the RXM-treated cells were binucleate. These findings demonstrate that RXM is a potent new modulator of cell cycle progression in HL60 cells and suggest that the inhibition of cytokinesis by this drug may provide a new model for studying mitosis. 相似文献
928.
929.
Abstract The ompK gene of Vibrio parahaemolyticus 1010 (RIMD 2210001) encoding an outer membrane protein (OMP), OmpK, which serves as the receptor for a broad-host-range vibriophage, KVP40, was cloned and sequenced. The gene consisted of 789 nucleotides encoding 263 amino acids. Since the first 20 amino acids most likely constitute the signal peptide, mature OmpK would consist of 243 amino acids with a calculated molecular mass of 27458 Da. Sequence comparisons indicate that OmpK is unique among Vibrio OMPs so far sequenced, but may be distantly related to Tsx of enteric bacteria and is homologous to an Aeromonas hydrophila OMP, protein IV. 相似文献