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31.
Takashi Nishinaka Yui Yamazaki Atsuko Niwa Hidenori Wake Shuji Mori Tadashi Yoshino 《Biomarkers》2020,25(3):305-313
AbstractBackground: Cerebral small vessel disease (CSVD) is associated with future stroke. Although pathological alteration in small vessels of patients with CSVD can be detected by neuroimaging, diagnosis of CSVD is delayed because it is an asymptomatic disease. The stroke-prone spontaneously hypertensive rat (SHRSP) show similar pathological features to human CSVD and develop stroke-related symptoms with advancing age.Objective: We investigated the time course of haematological parameters in Wistar rats and SHRSP.Material and Methods: Blood cells were analysed using an automated haematological analyser.Results: SHRSP develop stroke-related symptoms including onset of neurological symptoms, decreased body weight and blood brain barrier leakage between 12 and 14?weeks of age. Lymphocyte counts were gradually decreased at 3?weeks before development of stoke-related symptoms and then were further decreased after the development of stroke-related symptoms. The both mean platelet volume and large platelet ratio gradually increased at 3?weeks before the development of stoke-related symptoms. However, although SHRSP showed more microcytic red cells than Wistar rats, the trajectories of change in erythrocyte-related parameters were similar between Wistar rats and SHRSP.Conclusion: Our pilot study suggests that alterations of lymphocyte count and platelet volume predictive indicators for asymptomatic CSVD and symptomatic stroke in SHRSP. 相似文献
32.
Johnson JC Nettikadan SR Vengasandra SG Lovan S Muys J Henderson E Christiansen J 《In vitro cellular & developmental biology. Animal》2005,41(7):225-231
Summary Melanomacrophages (MMs) are a component of an internal, pigmented cell system in liver and splenic tissues of some fishes,
anurans, and reptiles. The cells have been found in centers or aggregates in sinusoids and are associated with cells capable
of producing a peptide cytokine and immunoglobulins. A unique cell extension process has been observed in turtle MMs placed
into cell culture, and this process has been studied by light and atomic force microscopy. These structures, referred to as
cablepodia, are uniquely straight, narrow, and unbranching and appear to originate from growth cones opposite lamellipodia.
Cablepodia were found to connect with other turtle MMs and fibroblasts forming cell networks. Dividing fibroblasts to which
a cablepodium attached ceased cell division. The observations collectively suggest that a principal reason for aggregations
of MMs in internal organs of lower vertebrates in their ability to form interconnected networks of cell processes for trapping
and processing of particulate matter, cells and infectious organisms and, possibly, for the communication of cell signals
and transfer of intracellular materials. 相似文献
33.
A new species of the genus Machilus Nees (Lauraceae) from China, M. parapauhoi F. N. Wei, S. C. Tang & W. B. Xu is described and illustrated. The new species is similar to M. pauhoi Kanehira, but differs in its densely appressed pubescent branchlets and petioles, shorter petiole, 0.8–1.2 cm long and an infructescence conspicuously shorter than the leaf. It is also similar to M. kwangtungensis Yang, but differs by its oblique leaves, with falcately curved apex and 14–18 lateral veins on both sides of the midrib. Based on a study of the type and isotype specimens of M. balansae (Airy Shaw) F. N. Wei & S. C. Tang and M. grandifolia S. Lee & F. N. Wei, M. grandifolia is regarded as a new synonym of M. balansae. 相似文献
34.
Dayanidhi Sarkar Masahiro Yabusaki Yuta Hasebe Pei Yee Ho Shuji Kohmoto Takayuki Kaga Kazuyuki Shimizu 《Applied microbiology and biotechnology》2010,87(1):127-136
The metabolism of Gluconacetobacter oboediens was investigated in relation to different carbon sources for the continuous cultures at the dilution rate of 0.05 h−1. The 13C-flux result implies the formation of metabolic recycles for the case of using glucose and acetate as carbon sources. When
glucose and ethanol were used as carbon sources, the specific ethanol uptake rate and the specific acetate production rate
increased as the feed ethanol concentration was increased from 40 to 60 g/l, while the specific CO2 production rate and the biomass concentration decreased, where the 13C-metabolic flux result indicates that the glycolysis, oxidative PP pathway, and the tricarboxylic acid (TCA) cycle were less
active, resulting in less biomass concentration. The flux result also implies that oxaloacetate decarboxylase flux became
negative, so that oxaloacetate is backed up by this pathway, resulting in less activity of glyoxylate pathway. When gluconate
was added for the case of using glucose and ethanol as carbon sources, the acetate and cell concentrations as well as gluconate
concentrations increased. The glucose and ethanol concentrations decreased concomitantly with the increased feed gluconate
concentration. In accordance with these fermentation characteristics, the enzyme activity result indicates that glucose dehydrogenase
and glucose-6-phosphate dehydrogenase pathways became less active, while the glycolysis and the TCA cycle was activated as
the feed gluconate concentration was increased. 相似文献
35.
Shuji Kitagawa Yasue Hongu Fujio Kametani 《Biochemical and biophysical research communications》1982,104(4):1371-1375
ADP-stimulated aggregation of bovine blood platelets was observed in media containing isotonic potassium salts of various monovalent anions. The aggregation depended on the anion in the medium, the order of aggregation being Cl?, Br?>I?>SCN?, . After 30-min incubation, the extent of aggregation of platelets in Cl? or Br? medium was little changed, whereas, that in SCN? or medium was remarkably decreased. This anion dependency of aggregation may be due to change in the membrane potential. 相似文献
36.
Rong Huang Sai Tian Rongrong Cai Jie Sun Wenqing Xia Xue Dong Yanjue Shen Shaohua Wang 《Journal of cellular and molecular medicine》2017,21(8):1448-1456
Saitohin (STH) Q7R polymorphism has been reported to influence the individual's susceptibility to Alzheimer's disease (AD); however, conclusions remain controversial. Therefore, we performed this meta‐analysis to explore the association between STH Q7R polymorphism and AD risk. Systematic literature searches were performed in the PubMed, Embase, Cochrane Library and Web of Science for studies published before 31 August 2016. Pooled odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to assess the strength of the association using a fixed‐ or random‐effects model. Subgroup analyses, Galbraith plot and sensitivity analyses were also performed. All statistical analyses were performed with STATA Version 12.0. A total of 19 case–control studies from 17 publications with 4387 cases and 3972 controls were included in our meta‐analysis. The results showed that the Q7R polymorphism was significantly associated with an increased risk of AD in a recessive model (RR versus QQ+QR, OR = 1.27, 95% CI = 1.01–1.60, P = 0.040). After excluding the four studies not carried out in caucasians, the overall association was unchanged in all comparison models. Further subgroup analyses stratified by the time of AD onset, and the quality of included studies provided statistical evidence of significant increased risk of AD in RR versus QQ+QR model only in late‐onset subjects (OR = 1.56, 95% CI = 1.07–2.26, P = 0.021) and in studies with high quality (OR = 1.37, 95% CI = 1.01–1.86, P = 0.043). This meta‐analysis suggests that the RR genotype in saitohin Q7R polymorphism may be a human‐specific risk factor for AD, especially among late‐onset AD subjects and caucasian populations. 相似文献
37.
38.
A subpopulation of bone marrow cells depleted by a novel antibody, anti-Liv8, is useful for cell therapy to repair damaged liver 总被引:26,自引:0,他引:26
Yamamoto N Terai S Ohata S Watanabe T Omori K Shinoda K Miyamoto K Katada T Sakaida I Nishina H Okita K 《Biochemical and biophysical research communications》2004,313(4):1110-1118
We previously reported a new in vivo model named as "GFP/CCl(4) model" for monitoring the transdifferentiation of green fluorescent protein (GFP) positive bone marrow cell (BMC) into albumin-positive hepatocyte under the specific "niche" made by CCl(4) induced persistent liver damage, but the subpopulation which BMCs transdifferentiate into hepatocytes remains unknown. Here we developed a new monoclonal antibody, anti-Liv8, using mouse E 11.5 fetal liver as an antigen. Anti-Liv8 recognized both hematopoietic progenitor cells in fetal liver at E 11.5 and CD45-positive hematopoietic cells in adult bone marrow. We separated Liv8-positive and Liv8-negative cells and then transplanted these cells into a continuous liver damaged model. At 4 weeks after BMC transplantation, more efficient repopulation and transdifferentiation of BMC into hepatocytes were seen with Liv8-negative cells. These findings suggest that the subpopulation of Liv8-negative cells includes useful cells to perform cell therapy on repair damaged liver. 相似文献
39.
Hu S Ni W Sai W Zhang H Cao X Qiao J Sheng J Guo F Chen C 《Biotechnology letters》2011,33(10):1949-1953
Myostatin is a negative regulator of skeletal muscle growth. Myostatin dysfunction therefore offers a strategy for promoting
animal muscle growth in livestock production. Knockdown of myostatin was achieved by combining RNA interference and the Sleeping
Beauty (SB) transposon system in sheep cells. Four targeting sites of sheep myostatin were designed and measured for myostatin
silencing in sheep fetal fibroblasts by real-time PCR. The sh3 construct induced significant decrease of myostatin gene expression
by 90% (P < 0.05). Myostatin silencing induced by SB-mediated sh3 was further tested in stably transfected cells. SB transposition
increased the integration frequency of genes into sheep genomes and mediated a more efficient myostatin knockdown than random
integration of sh3. We suggest that SB-mediated shRNA provides a novel potential tool for gene knockdown in the donor cells
of animal cloning. 相似文献
40.
Venkata Sai Prakash Chaturvedula John F. Clos Joshua Rhea Dennis Milanowski Ulla Mocek Grant E. DuBois Indra Prakash 《Phytochemistry letters》2011,4(3):209-212
From the commercial extract of the leaves of Stevia rebaudiana, three new diterpenoid glycosides were isolated besides eight known steviol glycosides including stevioside, rebaudiosides A–F and dulcoside A. The structures of the three compounds were identified as 13-[(2-O-β-d-glucopyranosyl-β-d-glucopyranosyl) oxy]-kaur-16-en-18-oic acid-(6-O-β-d-xylopyranosyl-β-d-glucopyranosyl) ester (1), 13-[(2-O-β-d-glucopyranosyl-β-d-glucopyranosyl) oxy]-17-hydroxy-kaur-15-en-18-oic acid β-d-glucopyranosyl ester (2), and 13-[(2-O-β-d-glucopyranosyl-β-d-glucopyranosyl) oxy]-17-oxo-kaur-15-en-18-oic acid β-d-glucopyranosyl ester (3) on the basis of extensive NMR and MS spectral studies. Another known diterpenoid glycoside, 13-[(2-O-β-d-glucopyranosyl-β-d-glucopyranosyl) oxy]-kaur-15-en-18-oic acid β-d-glucopyranosyl ester (4) was also isolated and its complete NMR spectral assignments were made on the basis of COSY, HSQC and HMBC spectral data. 相似文献