Comparisons of seasonal fluctuations in <Emphasis Type="Italic">Uroleucon nigrotuberculatum</Emphasis> (Hemiptera: Aphididae) densities between 2003–2004 and 2015–2016 in northern areas of its distribution range in Japan

Seasonal fluctuations in herbivore densities are affected by various factors. Solidago altissima was introduced from North America and has spread widely over Japan. Then, an aphid Uroleucon nigrotuberculatum and a lace bug Corythucha marmorata, both associated with S. altissima, invaded Japan in the late 1980s and 2000, respectively. In Miyagi, northeastern Japan, U. nigrotuberculatum and C. marmorata were established in the 1990s and 2012, respectively. We conducted periodic surveys of U. nigrotuberculatum in 2003–2004 and 2015–2016, and of C. marmorata in 2016 in Miyagi. Then, a competition experiment was performed to examine the relationship between them. In 2003, the average maximum daily temperature did not exceed 30 °C and the aphid was continuously confirmed on S. altissima during the census period. In other census years, the temperature exceeded 30 °C and the aphid disappeared in summer. At these sites, C. marmorata was continuously found on S. altissima. The presence of C. marmorata did not negatively affect U. nigrotuberculatum in the competition experiment. In addition, peak densities of U. nigrotuberculatum were higher in 2015–2016 than in 2003–2004. Based on these results, we conclude that U. nigrotuberculatum populations were at least partly affected by high temperature during the summer, but not by C. marmorata invasion in Miyagi.     

Occurrence of jasmonic acid in Dunaliella (Dunaliellales, Chlorophyta)

The occurrence of jasmonic acid and related compounds in Dunaliella species was investigated using gas-liquid chromatography/mass spectroscopy (GCY MS). Jasmonic acid was identified in the ethyl acetate soluble-acidic fraction of Dunaliella tertiolecta and Dunaliella salina (Dunal) Teodoresco, The concentration of jasmonic acid in D. salina. which is extremely halophilic, was much higher than that in D. tertiolecta Butcher, These results indicate that jasmonic acid might play an important role in salt-tolerance in Dunaliella.     

Preparation of a series of sulfated tetrasaccharides from shark cartilage chondroitin sulfate D using testicular hyaluronidase and structure determination by 500 MHz1H NMR spectroscopy

Six tetrasaccharide fractions were isolated from shark cartilage chondroitin sulfate D by gel filtration chromatography followed by HPLC on an amine-bound silica column after exhaustive digestion with testicular hyaluronidase. Their structures were determined unambiguously by one- and two-dimensional 500 MHz¹H NMR spectroscopy in conjunction with HPLC analysis of chondroitinase AC-II digests of the tetrasaccharides. One fraction was found to contain two tetrasaccharide components. All the seven tetrasaccharides shared the common core structure GlcA1-3GalNAc1-4GlcA1-3GalNAc with various sulfation profiles. Four were disulfated comprising of two monosulfated disaccharide units GlcA1-3GalNAc(4-sulfate) and/or GlcA1-3GalNAc(6-sulfate), whereas the other three were hitherto unreported trisulfated tetrasaccharides containing a disulfated disaccharide unit GlcA(2-sulfate)1-3GalNAc(6-sulfate) and a monosulfated disaccharide unit GlcA1-3GalNAc(4-or 6-sulfate). These sulfated tetrasaccharides were demonstrated to serve as appropriate acceptor substrates for serum -N-acetylgalactosaminyltransferase, indicating their usefulness as authentic oligosaccharide substrates or probes for the glycobiology of sulfated glycosaminoglycans.Abbreviations NFU National formulary unit - COSY correlation spectroscopy - HOHAHA homonuclear Hartmann-Hahn - 1D or 2D one- or two-dimensional - IdoA l-iduronic acid - GlcA d-gluco-4-enepyranosyluronic acid - Di-0S GlcA1-3GalNAc - Di-4S GlcA1-3GalNAc(4-sulfate) - Di-4S GlcA1-3GalNAc(4-sulfate) - Di-6S GlcA1-3GalNAc(6-sulfate) - Di-6S GlcA1-3GalNAc(6-sulfate) - Di-diS _d GlcA(2-sulfate)1-3GalNAc(6-sulfate) - Di-diS_E GlcA1-3GalNAc(4, 6-disulfate) - U G, U, 2S, 4S, and 6S represent GlcA, GalNAc, GlcA, 2-O-sulfate, 4-O-sulfate, and 6-O-sulfate, respectively     

The tyrosine-sorting motif of the vacuolar sorting receptor VSR4 from Arabidopsis thaliana,which is involved in the interaction between VSR4 and AP1M2, μ1-adaptin type 2 of clathrin adaptor complex 1 subunits,participates in the post-Golgi sorting of VSR4

μ1-Adaptin of adaptor protein (AP) 1 complex, AP1M, is generally accepted to load cargo proteins into clathrin-coated vesicles (CCVs) at the trans-Golgi network through its binding to cargo-recognition sequences (CRSs). Plant vacuolar-sorting receptors (VSRs) function in sorting vacuolar proteins, which are reportedly mediated by CCV. We herein investigated the involvement of CRSs of Arabidopsis thaliana VSR4 in the sorting of VSR4. The results obtained showed the increased localization of VSR4 at the plasma membrane or vacuoles by mutations in CRSs including the tyrosine-sorting motif YMPL or acidic dileucine-like motif EIRAIM, respectively. Interaction analysis using the bimolecular fluorescence complementation (BiFC) system, V10-BiFC, which we developed, indicated an interaction between VSR4 and AP1M2, AP1M type 2, which was attenuated by a YMPL mutation, but not influenced by an EIRAIM mutation. These results demonstrated the significance of the recognition of YMPL in VSR4 by AP1M2 for the post-Golgi sorting of VSR4.     

Effect of light exposure on linalool biosynthesis and accumulation in grape berries

Linalool is an important compound that contributes to the floral aroma in wines. This study showed the effect of light exposure on linalool accumulation in berries. The grape bunches were covered with films that block the full light spectrum (Shade) and the UV spectrum (UV-block), and a transparent film (Control). The linalool content was significantly higher in juice from Control-covered berries than in juice from Shade- and UV-block-covered berries, and the expression levels of the representative genes in linalool biosynthesis in Shade- and UV-block-covered berries were markedly lower than in Control-covered berries. These findings suggest that exposing berries to light is essential for linalool biosynthesis. To reflect sunlight onto grape clusters, reflective sheets were placed on the ground of a vineyard. The linalool content in berries exposed to sunlight reflected from the reflective sheets was higher than those in the control.     

Seasonality in mood and behaviours of Japanese residents in high-latitude regions: transnational cross-sectional study

Background

Daylight hours in high-latitude regions tend to be longer than those in Japan in summer, and shorter than those in Japan in winter. For example, daylight hours in London in winter are one-third those of Tokyo. Therefore, this study investigated and compared seasonal changes in mood and behaviours of Japanese individuals living in and outside Japan.

Methods

Surveys were conducted with Japanese residents in summer and winter in the UK (n?=?106), Nordic countries (n?=?40), Southeast Asia (n?=?50), and Japan (n?=?96). First, summer and winter General Health Questionnaire–28 (GHQ28) scores of each regional group were analysed. Subsequently, month-wise differences in mood and behaviours were compared across the four geographical regions.

Results

Summer and winter GHQ28 scores of participants living in the UK and Nordic countries differed significantly, while no seasonal differences were observed for residents in Japan and Southeast Asia. Further, in the UK and Nordic countries, summer was associated with better mood and more activity, while winter was linked to lowered mood and reduced activity.

Conclusion

The results indicate that Japanese living in the UK and Nordic countries (high-latitude regions) experience seasonal fluctuations in depressive symptoms that may be linked to drastic seasonal environmental changes. Observed over a 12-month period, their mood and behaviour declined in winter and improved in summer. Therefore, considering the prevalence of overseas stressors that differ from those in their home country, it is necessary to investigate the effectiveness of support systems that help migrants adapt to seasonal changes in high-latitude regions.

     

How the scrub height of dwarf pine Pinus pumila decreases at the treeline

Plant height decreases much within narrow altitudinal spans near treelines. We compared the stem age, stem inclination and shoot elongation rates of alpine dwarf pine Pinus pumila between the upper distribution limit (treeline, 2,850 m a.s.l.) and the lower distribution limit (2,500 m a.s.l.) on Mount Norikura in central Japan, to examine how the growth traits of P. pumila change with altitude. The mean stem height at the upper distribution limit (49 cm) was about a quarter of that at the lower distribution limit (187 cm). The mean ratio of stem height to length was lower at the upper distribution limit than at the lower distribution limit, indicating that P. pumila stems inclined more at the higher altitude. The mean stem age at the upper distribution limit (48 years) was less than a half of that at the lower distribution limit (109 years). Although the shoot elongation rate positively correlated with stem length at the two altitudes, the shoot elongation rate at a given stem length was lower at the upper distribution limit than at the lower distribution limit. Thus, less developed scrub at the upper distribution limit than at the lower distribution limit was due to shorter stem age, more creeping stems and lower shoot elongation rates. Generally, wind velocity is greater in higher altitudes. Probably, strong wind reduces the growth and mean stem age of P. pumila stems at the upper distribution limit. Therefore, this study concludes that the scrub height of P. pumila is controlled not only by temperature, but also by strong wind.     

Ranging patterns of Japanese macaques in the coniferous forest of Yakushima: Home range shift and travel rate

Ranging is one of the most important behavioral adaptations for coping with seasonally fluctuating food and thermal conditions. We studied the ranging patterns, in particular home range shift and travel rate of Japanese macaques (Macaca fuscata) in the coniferous forest of Yakushima by tracking a group for 17 months. We also supplemented our data with records collected every August over a 5-year annual census. The macaque group used the eastern part of their home range from May to September and the western part during the rest of the year. The eastern part of the home range was largely primary forest in the national park, and the altitude was higher than in the western part. When they used the western part, the macaques ate more herbs and fruits from small-sized trees, the availability of which was higher in the logged forest. This east–west home range shift occurred repeatedly over multiple years. A neighboring group occupied the western part of the focal group's home range in summer, which the focal group did not use in that season. Both temperature and diet affected seasonal changes in the monthly average travel rate. Animals need more energy for thermoregulation when the temperature is low, so the macaques decreased their ranging efforts to save energy at times of low temperature. They increased their ranging distance to eat fungi, since their encounters with this food would increase with the total distance walked. They also increased their travel rate when eating flowers, which had lower food patch (tree) density than other foods such as fruits. The data supported the hypothesis that the macaques capitalize on habitat heterogeneity in a seasonally fluctuating habitat by shifting their home range and modifying their travel rate.     

Coumarin and Euponin,Two Inhibitors for Insect Development from Leaves of Eupatorium japonicum

An ovicide and a larval growth inhibitor against Drosophila melanogaster were isolated from leaves of Eupatorium japonicum. The ovicide was identified as coumarin. The structure of the larval growth inhibitor, a new naturally occurring guaianolide named euponin, was elucidated on the basis of spectral and chemical evidence. The inhibitory effects of these agents on the egg and larva were also described.