Structural-functional characteristics of Yersinia pseudotuberculosis plasmid pVM82

The restriction map of Yersinia pseudotuberculosis plasmid pVM82 was established using the "chromosome walking" method. According to transpositional mutagenesis, the plasmid pVM82 appeared to be conjugative and was able to be transmitted from Y. pseudotuberculosis to the E. coli K-12 cells.     

Analysis of the plasmid composition of Yersinia pseudotuberculosis strains and its use for typing pseudotuberculosis pathogens

Electrophoresis in agarose gel has been used to study the plasmid spectra of 854 Yersinia pseudotuberculosis strains isolated from different sources. The plasmids found in the microbial strains are represented by the elements with molecular masses 82; 57; 45; 5.5; 4.4; 3.5; 2.7; 2.4; 2.3 Md. The variable spectra of plasmids is peculiar only for serovar I of Yersinia pseudotuberculosis. Plasmids p45 and p82 are classified as the main, while other plasmids as auxiliary ones. In accord with the classification all plasmid containing strains are divided into 8 plasmid strains. Using the proposed method for intraspecific typing of Yersinia pseudotuberculosis permits one to perfect the epidemiological analysis of pseudotuberculosis infection and make concrete the direction of prophylactic and antiepidemic measures.     

Habitat selection of breeding brown eared-pheasants (Crossoptilon mantchuricum) in Xiaowutaishan National Nature Reserve, Hebei Province, China

From April to July of 2003–2005, we investigated habitat selection of breeding brown eared-pheasants (Crossoptilon mantchuricum) in the Xiaowutaishan National Nature Reserve, Hebei Province. Our results show that brown eared-pheasants preferred deciduous-coniferous mixed or deciduous forests with altitudes ranging from 1600 to 2200 m, steep gradients of slopes and medium or high positions on slopes. Moreover, they preferred habitats with more tree species, larger diameters at the breast height, more density and cover of trees and habitats with more types of grasses, high density and cover of grasses, but with fewer types of shrubs and lower density and cover of shrubs. They had larger territories in the early breeding stage than in the late breeding stage. There were significant differences in diameters at the breast height of trees, average height of trees, cover of trees, density of shrubs, cover of shrubs, aspect, distance to path, distance to water, distance to forest edge (P < 0.01, respectively), density of trees, type of shrubs, height of shrubs, type of grasses, density of grasses and proportion of bareness (P < 0.05, respectively). The result of the principal component analysis suggested that the principal components among the 23 habitat factors were the average height of trees, density of trees, diameters at the breast height of trees, density of shrubs, cover of shrubs and density of grasses. The differences in habitat selection of brown eared-pheasants between early breeding and late breeding stages may correlate with the dynamics of ambient environmental conditions, the reproductive behavior and energy requirements in their sub-stages of breeding.     

Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation

Plant phosphate (Pi) transporters mediate the uptake and translocation of this nutrient within plants. A total of 13 sequences in the rice ( Oryza sativa ) genome can be identified as belonging to the Pi transporter (Pht1) family. Here, we report on the expression patterns, biological properties and the physiological roles of two members of the family: OsPht1;2 ( OsPT2 ) and OsPht1;6 ( OsPT6 ). Expression of both genes increased significantly under Pi deprivation in roots and shoots. By using transgenic rice plants expressing the GUS reporter gene, driven by their promoters, we detected that OsPT2 was localized exclusively in the stele of primary and lateral roots, whereas OsPT6 was expressed in both epidermal and cortical cells of the younger primary and lateral roots. OsPT6, but not OsPT2, was able to complement a yeast Pi uptake mutant in the high-affinity concentration range. Xenopus oocytes injected with OsPT2 mRNA showed increased Pi accumulation and a Pi-elicited depolarization of the cell membrane electrical potential, when supplied with mM external concentrations. Both results show that OsPT2 mediated the uptake of Pi in oocytes. In transgenic rice, the knock-down of either OsPT2 or OsPT6 expression by RNA interference significantly decreased both the uptake and the long-distance transport of Pi from roots to shoots. Taken together, these data suggest OsPT6 plays a broad role in Pi uptake and translocation throughout the plant, whereas OsPT2 is a low-affinity Pi transporter, and functions in translocation of the stored Pi in the plant.     

The phosphate transporter gene OsPht1;8 is involved in phosphate homeostasis in rice

Plant phosphate transporters (PTs) are active in the uptake of inorganic phosphate (Pi) from the soil and its translocation within the plant. Here, we report on the biological properties and physiological roles of OsPht1;8 (OsPT8), one of the PTs belonging to the Pht1 family in rice (Oryza sativa). Expression of a β-glucuronidase and green fluorescent protein reporter gene driven by the OsPT8 promoter showed that OsPT8 is expressed in various tissue organs from roots to seeds independent of Pi supply. OsPT8 was able to complement a yeast Pi-uptake mutant and increase Pi accumulation of Xenopus laevis oocytes when supplied with micromolar (33)Pi concentrations at their external solution, indicating that it has a high affinity for Pi transport. Overexpression of OsPT8 resulted in excessive Pi in both roots and shoots and Pi toxic symptoms under the high-Pi supply condition. In contrast, knockdown of OsPT8 by RNA interference decreased Pi uptake and plant growth under both high- and low-Pi conditions. Moreover, OsPT8 suppression resulted in an increase of phosphorus content in the panicle axis and in a decrease of phosphorus content in unfilled grain hulls, accompanied by lower seed-setting rate. Altogether, our data suggest that OsPT8 is involved in Pi homeostasis in rice and is critical for plant growth and development.     

Tomato sugar transporter genes associated with mycorrhiza and phosphate

A new kind of ribosome-inactivating protein (curcin 2), induced by several different kinds of stress from Jatropha curcas leaves, under the control of the CaMV (cauliflower mosaic virus) 35S promoter, was introduced into the tobacco genome by Agrobacterium tumefaciens-mediated transformation method. The curcin 2 protein was only detected in the transgenic tobacco plantlets transformed with the cur2p fragment (coding premature curcin 2 protein), but not in the plantlets with the cur2m fragment (coding mature curcin 2 protein). The T1 population of the transgenic lines shows an increased tolerance to tobacco mosaic virus (TMV) and a fungal pathogen Rhizoctonia solani by delaying the development of systemic symptoms of TMV and reducing the damage caused by the fungal disease. The increases of the tolerances correspond to the curcin 2 level in the transgenic plants.     

Glucocorticoids synergize with IL-1β to induce TLR2 expression via MAP Kinase Phosphatase-1-dependent dual Inhibition of MAPK JNK and p38 in epithelial cells

Background  

Despite the importance of glucocorticoids in suppressing immune and inflammatory responses, their role in enhancing host immune and defense response against invading bacteria is poorly understood. Toll-like receptor 2 (TLR2) has recently gained importance as one of the major host defense receptors. The increased expression of TLR2 in response to bacteria-induced cytokines has been thought to be crucial for the accelerated immune response and resensitization of epithelial cells to invading pathogens.