山羊关节炎─脑炎前病毒的PCR检测

山羊关节炎─脑炎前病毒的ＰＣＲ检测相文华,丁恩雨,秦运安,吕晓玲,沈荣显（中国农业科学院哈尔滨兽医研究所哈尔滨１５０００１）关键词聚合酶链反应,山羊关节炎—脑炎病毒,整合山羊关节炎—脑炎（ＣａｐｒｉｎｅＡｒｔｈｒｉｔｉｓ－Ｅｎｃｅｐｈａｌｉｔｉｓ,Ｃ...     

经颅多普勒超声对80例偏头痛病人的检测分析

经颅多普勒超声波对８０例偏头痛病人与１１０例正常对照组血流速度进行检测．结果发现病例组血流速度异常为８７．５％。根据血流速度改变,本组分为血流速度增快型与血流速度减慢型,这有助于偏头痛的临床诊断。     

对似近分析的一点改进及其拓广

本文给出了似近分析一个重要的改进,并将改进的似近分析推广为m维似近分析,同时给出了两个数学定理及其证明。根据多维似近系数的数学性质,提出了一种新的聚类方法,即逐步多维聚类法。实例分析证明,这种聚类法比二维系数聚类优越,聚类功能强,所获得的结果更为客观。     

油菜菌核病菌生理生态研究

通过试验分析,结果得出,油菜菌核病菌生长温度范围在0～34℃之间,23℃以下菌丝生长速度与温度呈正相关,25℃以上则呈负相关,23～25℃最适宜,菌丝和菌核致死温度分别为45℃和75℃．该菌对酸碱度适应范围很广,PH1～14范围内只在PH_1条件下不能生长,其最适PH值为5～6。病菌利用铵态氮源能力最强,硝态氮源次之,不能利用亚硝态氮;对氨基酸利用能力以精氨酸最强,其次是酪氨酸、天门冬氨酸和谷氨酸,赖氨酸最差。植株内精氨酸和赖氨酸比值大的白菜型油菜“黄鳝籽”病情扩展最快。     

广义捕食系统无闭轨的几组充分条件

本文对一类广义捕食系统生物种群生态常微模型建立几组无闭轨的充分条件,为开拓涉及该类模型制作参考类作全局制图定性的研究．     

人红细胞NADH－细胞色素b5还原酶的分离纯化与鉴定

 人红细胞ＮＡＤＨ－细胞色素ｂ５还原酶的分离纯化与鉴定黄长晖,朱忠勇,唐玉钗（南京军区福州总院全军医学检验中心实验科,福州３５０００１）ＮＡＤＨ细胞色素ｂ５还原酶（Ｃｙｔｂ５Ｒ,Ｅ．Ｃ．１．６．２．２．）是红细胞内催化高铁血红蛋白还原为血红蛋白的关键酶...     

CBP-18, a Ca2+-Binding Protein in Rat Brain: Tissue Distribution and Localization

Abstract: The distribution of a novel calcium-binding protein with a molecular mass of 18 kDa (CBP-18) in the rat brain was studied by means of biochemical methods and immunohistochemistry on cryostat-sectioned tissue and compared with staining patterns of parvalbumin on adjacent sections. The biochemical analysis revealed high levels of CPB-18 in cortex and cerebellum, low levels in the lungs, and undetectable levels in all other tissues tested. Immunohistochemically, the polyclonal rabbit-derived antibody for CPB-18 showed selective affinity with periglomerular cells and dendrites in the olfactory bulb. Distinct immunostaining of scattered cells and their proximal dendrites was found in the anterior olfactory nuclei and in the perirhinal and entorhinal cortex. Strong staining of neuropil with recognizable but diffusely outlined cells was observed in the retrosplenial cortex, central amygdala, hippocampal rudiment, septum, area preoptica, hypothalamus, colliculus superior, and parabrachial nuclei. The cerebellum showed strong neuropil staining of both the molecular and the granule cell layer. Less intense neuropil staining and a few scattered cells were found in the neocortex, the remaining basal forebrain, and in the entire brainstem. Immunoreactivity was barely detectable or missing in the striatum, the hippocampus, the thalamus, and in the colliculus inferior. Thus, CPB-18 shows a unique staining pattern in the CNS, different from all other Ca²⁺-binding proteins studied so far.     

Productive infection of a cervical epithelial cell line with human immunodeficiency virus: implications for sexual transmission.

The human cervix-derived epithelial cell line (ME180) used in this study displays a characteristics epithelial morphology, including numerous desmosomes, tonofilaments, and epidermal filaments. When T-cell lines infected with human immunodeficiency virus (HIV) are added to epithelial cultures, they rapidly adhere to the epithelial monolayer. Within a few minutes, the T cells shed numerous virions into narrow spaces formed between the epithelial cell and the adherent T cells. Virions subsequently enter the ME180 cells via large vesicles. A few days after infection, cytopathic effects and syncytium formation were observed. Infected clones of ME180 cells have remained infected for 8 months. p24 enzyme-linked immunosorbent assay and infectivity assays show that one subclone of the cell line produces virus titers equivalent to those of high-secreting HIV-infected T-cell lines. Electron microscopy reveals numerous virions budding from both the basal and apical surfaces of the epithelium. These observations suggest that cervical epithelium has the potential to serve as a site of HIV infection.     

分散角质层的分类和命名*

简略讨论了分散角质层对地层学、古生物学和古环境的意义;简述了气孔器的形态学、发生学和形态一发生学的分类;在介绍并讨论了分散角质层的分类原则和命名方法的基础上,提出了新的分类方案.     

Degradation and debittering of a tryptic digest from beta-casein by aminopeptidase N from Lactococcus lactis subsp. cremoris Wg2.

The mode of action of purified aminopeptidase N from Lactococcus lactis subsp. cremoris Wg2 on a complex peptide mixture of a tryptic digest from bovine beta-casein was analyzed. The oligopeptides produced in the tryptic digest before and after aminopeptidase N treatment were identified by analysis of the N- and C-terminal amino acid sequences and amino acid compositions of the isolated peptides and by on-line liquid chromatography-mass spectrometry. Incubation of purified peptides with aminopeptidase N resulted in complete hydrolysis of many peptides, while others were only partially hydrolyzed or not hydrolyzed. The tryptic digest of beta-casein exhibits a strong bitter taste, which corresponds to the strong hydrophobicity of several peptides in the tryptic digest of beta-casein. The degradation of the "bitter" tryptic digest by aminopeptidase N resulted in a decrease of hydrophobic peptides and a drastic decrease of bitterness of the reaction mixture.