区域生态经济型防护林体系建设模式智能决策支持系统的研制开发

根据生态经济型防护林体系及其建设模式的内涵,研制开发出了以专家为核心,包括数据及数据库、图形及图形库、模型及模型库等模块的智能决策支持系统,以便为给定区域提供有关生态经济型防护林体系建设方面的决策服务。     

Differential gene expression in salt-tolerant rice mutant and its parental variety

The differential expressions of three genes rbcL, salT and rab!6 in response to ABA, NaCl, PEG and heat shock were investigated in seedlings of a salt-tolerant rice mutant 20 (mutant 20) and its parental variety Oryza sativa var. japonica 77-170(170). By Northern blot analysis it was found that ABA induced the expression of all three genes of rbcL, salT and rab16 in shoots and roots of both 170 and mutant 20 with the exceptions of rab16 in shoots of mutant 20 and rbcL in roots of 170. Lower concentrations of NaCl induced rbcL expression in shoots of mutant 20 but not 170. Higher concentrations of NaCl decreased rbcL expression but induced expressions of salT and rab16 in shoots of both 170 and mutant 20. PEG(15%) and 37℃ heat shock showed almost no effects on the expression of the three genes in mutant 20. However, they caused a decrease in rbcL expression and slight induction of the rab16 gene in 170, with salT expression unaffected. These results indicated that mutant 20 was relatively less responsiv     

Phytogeny of 12 species of genus Glycine Willd. reconstructed with internal transcribed region in nuclear ribosomal DNA

The ITS-Is of 24 accessions belong to 10 species of subgenus Glycine, and 2 species of subgenus Soja of genus Glycine were amplified, cloned and sequenced. According to the homology of the sequences, the phy-logeny of the 24 accessions were reconstructed. The reconstructed dendrogram showed that there were some divergent genomic types found in the previously classified species, such as G . tomentella, G. canescens and G. tabacina, and they might be some cryptic species by morphologic analysis.     

Characterization of Humoral Immune Responses against Capsid Protein p24 and Transmembrane Glycoprotein gp41 of Human Immunodeficiency Virus Type 1 in China

The objective of this study was to extend our previous research and to further characterize the humoral immune responses against HIV-1 p24, gp41 and the specific peptides carrying the immunodominant epitopes (IDEs) that react with human serum samples from HIV-1-infected individuals in China. We found that the majority (90.45%, 180/199) of the samples did not react with any of the three HIV-1 p24 peptides carrying IDEs, but did react with the recombinant full-length p24, suggesting that these samples tested in China were primarily directed against the conformational epitopes of HIV-1 p24. In contrast, 84.54% (164/194) of the samples reacted with at least one HIV-1 linear gp41 peptide, in particular the gp41-p1 peptide (amino acids 560–616). Both recently and long-term HIV-1-infected individuals displayed similar humoral immune responses against the recombinant gp41. However, samples from long-term HIV-1-infected subjects but not from recently infected subjects, showed a very strong reaction against the gp41-p1 peptide. The different response patterns observed for the two groups against the gp41 and the peptide gp41-p1 were statistically significant (P<0.01, Chi-square test). These results have direct relevance and importance for design of improved HIV-1 p24 detection assays and the gp41- based immunoassay that can be used to reliably distinguish recent and long-term HIV-1 infection.     

Comparative study of QTLs for agronomic traits of riceOriza sativa L.) between salt stress and nonstress environment

Genotype-by-environment interactions (GxE) are commonly observed for quantitative traits. In the present study, a doubled haploid (DH) population and its genetic linkage map were used to comparatively study QTLs in salt stress and nonstress environments. A total of 24 QTLs were detected for five agronomic traits, which were distributed on all the chromosomes except 9 and 11. Under the salt stress, nine (37.5%) QTLs were detected, including one for 1 000-grain weight (GW), two for heading date (HD), one for plant height (PH), two for grains per panicle (GPP), and three for effective tillers (ET), while in the nonstress environment, 17 QTLs (70.8%) were detected, including five for GW, six for HD, three for PH, two for GPP, and one for ET. Two QTLs (8.3%) were consistently detected in both environments. One was identified on chromosome 4 for HD and the other on Chr.6 for GPP. Furthermore, three regions carrying multiple QTLs were identified on chromosomes 1, 4 and 8 respectively. For example, on chromosome 8, three QTLs for HD, GW and PH, respectively were identified between RG885-GA408 in nonstress environment, but not in the stress environment. The comparative study of QTLs detected in extremely different (salt stress and nonstress) environments revealed that there existed several QTLs for important agronomic traits on chromosome 8 which were affected significantly by salt stress.     

灰树花液体深层培养工艺研究

对灰树花进行了液体深层培养试验,结果表明：液体培养基碳源以豆粉+麸皮、玉米粉+麸皮和氮源以蛋白胨、牛肉膏等较适宜菌丝生长的物质,确定了灰树花深层培养的液体培养基配方;并研究了培养条件对菌丝生物量的影响,得出了培养液pH在5．30～6．00和通气量为1：(1．00～1．40)的条件下,菌丝生物量达到最大。