全文获取类型
收费全文 | 616篇 |
免费 | 46篇 |
出版年
2023年 | 2篇 |
2022年 | 6篇 |
2021年 | 24篇 |
2020年 | 21篇 |
2019年 | 8篇 |
2018年 | 20篇 |
2017年 | 20篇 |
2016年 | 21篇 |
2015年 | 33篇 |
2014年 | 47篇 |
2013年 | 41篇 |
2012年 | 42篇 |
2011年 | 43篇 |
2010年 | 22篇 |
2009年 | 28篇 |
2008年 | 35篇 |
2007年 | 20篇 |
2006年 | 15篇 |
2005年 | 15篇 |
2004年 | 25篇 |
2003年 | 12篇 |
2002年 | 15篇 |
2001年 | 15篇 |
2000年 | 9篇 |
1999年 | 11篇 |
1998年 | 7篇 |
1996年 | 6篇 |
1995年 | 6篇 |
1994年 | 8篇 |
1993年 | 2篇 |
1992年 | 5篇 |
1991年 | 9篇 |
1990年 | 8篇 |
1989年 | 7篇 |
1988年 | 6篇 |
1987年 | 3篇 |
1986年 | 5篇 |
1985年 | 4篇 |
1984年 | 7篇 |
1981年 | 3篇 |
1979年 | 3篇 |
1978年 | 4篇 |
1977年 | 1篇 |
1975年 | 1篇 |
1974年 | 4篇 |
1973年 | 3篇 |
1972年 | 2篇 |
1970年 | 1篇 |
1969年 | 1篇 |
1966年 | 2篇 |
排序方式: 共有662条查询结果,搜索用时 0 毫秒
661.
Rena Matsuura Mayumi Kishida Rie Konishi Yuuki Hirata Noriko Adachi Shota Segawa Kenta Imao Tsutomu Tanaka Akihiko Kondo 《Biotechnology and bioengineering》2019,116(10):2640-2651
Microbial production of 1,5-diaminopentane (DAP) from renewable feedstock is a promising and sustainable approach for the production of polyamides. In this study, we constructed a β-glucosidase (BGL)-secreting Corynebacterium glutamicum and successfully used this strain to produce DAP from cellobiose and glucose. First, C. glutamicum was metabolically engineered to produce l -lysine (a direct precursor of DAP), followed by the coexpression of l -lysine decarboxylase and BGL derived from Escherichia coli and Thermobifida fusca YX (Tfu0937), respectively. This new engineered C. glutamicum strain produced 27 g/L of DAP from cellobiose in CGXII minimal medium using fed-batch cultivation. The yield of DAP was 0.43 g/g glucose (1 g of cellobiose corresponds to 1.1 g of glucose), which is the highest yield reported to date. These results demonstrate the feasibility of DAP production from cellobiose or cellooligosaccharides using an engineered C. glutamicum strain. 相似文献
662.
Hideki Tanaka Hirotsugu Hino Shota Moriya Hiromi Kazama Masaya Miyazaki Naoharu Takano Masaki Hiramoto Kiyoaki Tsukahara Keisuke Miyazawa 《Biochemistry and Biophysics Reports》2020
Tyrosine kinase inhibitors (TKIs) induce autophagy in many types of cancer cells. We previously reported that gefitinib (GEF) and imatinib (IMA) induce autophagy in epidermal growth factor receptor (EGFR) knock-out A549 and non-BCR-ABL-expressing leukemia cell lines, respectively. This evidence suggests that TKI-induced autophagy is independent of the original target molecules. The present study compared the autophagy-inducing abilities of various TKIs, regardless of their targets, by quantitative autophagy flux assay. We established stable clones expressing the GFP-LC3-mCherry-LC3ΔG plasmid in A549, PC-9, and CAL 27 cell lines and assessed autophagy inducibility by monitoring the fluorescent ratios of GFP-LC3 to mCherry-LC3ΔG using an IncuCyte live cell imaging system during exposure to TKIs viz; GEF, osimertinib (OSI), lapatinib (LAP), lenvatinib (LEN), sorafenib (SOR), IMA, dasatinib (DAS), and tivantinib (TIV). Among these TKIs, DAS, GEF, and SOR exhibited prominent autophagy induction in A549 and PC-9 cells. In CAL 27 cells, IMA, SOR, and LEN, but not GEF, TIV, or OSI, exhibited autophagy induction. In the presence of azithromycin (AZM), which showed an inhibitory effect on autophagy flux, TKIs with prominent autophagy inducibility exhibited enhanced cytotoxicity via non-apoptotic cell death relative to effects of TKI alone. Therefore, autophagy inducibility of TKIs differed in the context of cancer cells. However, once induced, they appeared to have cytoprotective functions. Thus, blocking TKI-induced autophagy with AZM may improve the therapeutic effect of TKIs in cancer cells. 相似文献