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161.
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163.
Kubota Yasuhiko Iso Hiroyasu Ikehara Satoyo Tamakoshi Akiko 《Sleep and biological rhythms》2015,13(1):85-93
Sleep and Biological Rhythms - Sleep duration could affect glucose tolerance and mortality. However, the impact that sleep duration has on prognosis of people with diabetes is unclear. A cohort of... 相似文献
164.
We used a particle-based Monte Carlo simulation to dissect the regulatory mechanism of molecular translocation of CaMKII,
a key regulator of neuronal synaptic function. Geometry was based upon measurements from EM reconstructions of dendrites in
CA1 hippocampal pyramidal neurons. Three types of simulations were performed to investigate the effects of geometry and other
mechanisms that control CaMKII translocation in and out of dendritic spines. First, the diffusional escape rate of CaMKII
from model spines of varied morphologies was examined. Second, a postsynaptic density (PSD) was added to study the impact
of binding sites on this escape rate. Third, translocation of CaMKII from dendrites and trapping in spines was investigated
using a simulated dendrite. Based on diffusion alone, a spine of average dimensions had the ability to retain CaMKII for duration
of ~4 s. However, binding sites mimicking those in the PSD controlled the residence time of CaMKII in a highly nonlinear manner.
In addition, we observed that F-actin at the spine head/neck junction had a significant impact on CaMKII trapping in dendritic
spines. We discuss these results in the context of possible mechanisms that may explain the experimental results that have
shown extended accumulation of CaMKII in dendritic spines during synaptic plasticity and LTP induction. 相似文献
165.
Ishiuchi K Kubota T Ishiyama H Hayashi S Shibata T Mori K Obara Y Nakahata N Kobayashi J 《Bioorganic & medicinal chemistry》2011,19(2):749-753
Three new Lycopodium alkaloids, lyconadins D (1) and E (2), and complanadine E (3), were isolated from the club moss Lycopodium complanatum. Lyconadin D (1) was the first example of fastigiatine-type alkaloid isolated from Lycopodium complanatum. The structures and relative stereochemistry of 1-3 were elucidated on the basis of spectroscopic data. Complanadine E (3) enhanced mRNA expression for NGF. 相似文献
166.
Polyglutamine (polyQ)-expansion proteins cause protein aggregation in the cytosol and nucleus of neuronal cells, leading to neurodegenerative diseases. For example, expansion of the polyQ tract (>40 repeats) in huntingtin (htt) proteins leads to Huntington disease, while polyQ-expanded ataxins cause several types of ataxias. PolyQ-rich inclusions are found in neuronal cells of patients, suggesting that polyQ disease is caused by protein misfolding. However, the mechanisms by which polyQ-expansion proteins exert neuronal toxicity are largely unknown. Here, we review experimental procedures to analyze the roles of molecular chaperones in preventing polyQ aggregation and toxicity as well as to measure the characteristics and dynamics of polyQ aggregation, particularly focusing on cellular models and dynamic imaging of fluorescently-labeled polyQ-expansion proteins and their modulation by chaperones. 相似文献
167.
Suehara Y Tochigi N Kubota D Kikuta K Nakayama R Seki K Yoshida A Ichikawa H Hasegawa T Kaneko K Chuman H Beppu Y Kawai A Kondo T 《Journal of Proteomics》2011,74(6):829-842
We aimed to develop prognostic biomarkers for synovial sarcoma employing a proteomic approach. We examined the proteomic profile of synovial sarcoma using two-dimensional difference gel electrophoresis (2D-DIGE). We identified 20 protein spots whose intensity was statistically different (p<0.01) between a group of eight patients who were alive and continuously disease-free for over five years and a group of five patients who died of the disease within two years post diagnosis. Mass spectrometric protein identification demonstrated that these 20 spots corresponded to 17 distinct gene products. Three of the 20 spots corresponded to secernin-1 and had higher intensity in the good prognosis group. The prognostic performance of secernin-1 was further examined immunohistochemically in 45 synovial sarcoma cases. The 5-year survival rate was 77.6% and 21.8% for patients with secernin-1 positive and negative primary tumors respectively (p=0.0015). The metastasis-free survival was significantly higher in the patient group with high secernin-1 expression compared to that with low expression (p=0.0012). Uni- and multivariate analyses revealed that secernin-1 expression was a powerful prognostic factor compared to other clinico-pathological parameters examined. These results indicate that secernin-1 may be used as a biomarker to predict the overall and metastasis-free survival in synovial sarcoma patients. 相似文献
168.
Ohgawara T Kubota S Kawaki H Kurio N Abd El Kader T Hoshijima M Janune D Shimo T Perbal B Sasaki A Takigawa M 《Journal of cell communication and signaling》2011,5(4):291-299
The CCN family of proteins consists of six members with conserved structural features. These proteins play several roles in the physiology and pathology of cells. Among the pathological roles of the CCN family, one of the most important and controversial ones is their role in the expansion and metastasis of cancer. Up to now a number of reports have described the possible role of each CCN family member independently. In this study, we comprehensively analyzed the roles of all six CCN family members in cell growth, migration and invasion of breast cancer cells in vitro and in vivo. As a result, we found the CCN2/CCN3 ratio to be a parameter that is associated with the metastatic phenotype of breast cancer cells that are highly metastatic to the bone. The same analysis with cell lines from oral squamous carcinomas that are not metastatic to the bone further supported our notion. These results suggest the functional significance of the interplay between CCN family members in regulating the phenotype of cancer cells. 相似文献
169.
S. Kubota H. Yeger B. Perbal M. Takigawa 《Journal of cell communication and signaling》2011,5(1):9-24
Abstracts
Abstracts of the 6th International Workshop on the CCN Family of Genes Held at the Slieve Donnard Hotel, Newcastle, Northern Ireland 20–24 October 2010 相似文献170.
Mushroom bodies (MB) are insect brain centers involved in learning and other complex behaviors and they are particularly large in ants. We describe the larval and pupal development of the MB in the carpenter ant, Camponotus japonicus. Based on morphological cues, we characterized the stages of preimaginal development of worker ants. We then describe morphological changes and neurogenesis underlying the MB development. Kenyon cells are produced in a proliferation cluster formed by symmetrical division of MB neuroblasts. While the duration of larval instars shows great individual variation, MB neuroblasts increase in number in each successive larval instar. The number of neuroblasts increases further during prepupal stages and peaks during early pupal stages. It decreases rapidly, and then neurogenesis generally ceases during the mid pupal stage (P4). In contrast to the larval period, the MB development of individuals is highly synchronized with physical time throughout metamorphosis. We show that carpenter ants (C. japonicus) have approximately half as many MB neuroblasts than are found in the honey bee Apis mellifera. Mature MBs of carpenter ants and honey bees reportedly comprise almost the same number of neurons. We therefore suggest that the MB neuroblasts in C. japonicus divide more often in order to produce a final number of MB neurons similar to that of honey bees. 相似文献