全文获取类型
收费全文 | 1996篇 |
免费 | 96篇 |
国内免费 | 2篇 |
专业分类
2094篇 |
出版年
2022年 | 10篇 |
2021年 | 23篇 |
2020年 | 13篇 |
2019年 | 13篇 |
2018年 | 20篇 |
2017年 | 12篇 |
2016年 | 49篇 |
2015年 | 61篇 |
2014年 | 79篇 |
2013年 | 139篇 |
2012年 | 100篇 |
2011年 | 94篇 |
2010年 | 64篇 |
2009年 | 63篇 |
2008年 | 106篇 |
2007年 | 101篇 |
2006年 | 108篇 |
2005年 | 90篇 |
2004年 | 95篇 |
2003年 | 94篇 |
2002年 | 107篇 |
2001年 | 39篇 |
2000年 | 46篇 |
1999年 | 40篇 |
1998年 | 29篇 |
1997年 | 20篇 |
1996年 | 20篇 |
1995年 | 27篇 |
1994年 | 20篇 |
1993年 | 20篇 |
1992年 | 30篇 |
1991年 | 28篇 |
1990年 | 28篇 |
1989年 | 29篇 |
1988年 | 17篇 |
1987年 | 19篇 |
1986年 | 13篇 |
1985年 | 18篇 |
1984年 | 11篇 |
1983年 | 16篇 |
1982年 | 13篇 |
1981年 | 25篇 |
1980年 | 21篇 |
1979年 | 13篇 |
1978年 | 15篇 |
1977年 | 12篇 |
1976年 | 13篇 |
1975年 | 16篇 |
1974年 | 14篇 |
1973年 | 10篇 |
排序方式: 共有2094条查询结果,搜索用时 0 毫秒
171.
172.
The interaction of saponins with phospholipid vesicles was investigated by means of liposomal agglutination or a precipitation assay. Ginsenoside-Rc, which has an α-l-arabinofuranose residue at the non-reducing terminus, exhibited remarkable agglutinability toward egg yolk phosphatidylcholine vesicles, while other saponins lacking this characteristic sugar residue showed less or no agglutinability. The molar ratio of ginsenoside-Rc to egg phosphatidylcholine in the aggregates was estimated to be 0.4–0.5 by a precipitation assay using 14C-labeled egg phosphatidylcholine vesicles. The agglutination was inhibited by p-nitrophenyl α-l-arabinofuranoside but not by p-nitrophenyl β-d-glucopyranoside or arabinogalactan. The results indicated that the α-l-arabinofuranose residue in ginsenoside-Rc should be important for the expression of the agglutinability. The agglutinability of ginsenoside-Rc toward lipid vesicles depended on both the polar head groups and fatty acyl chains of phospholipids. Egg yolk phosphatidylcholine vesicles were strongly agglutinated by ginsenoside-Rc, although sphingomyelin, phosphatidylethanolamine, phosphatidic acid and phosphatidylserine were less agglutinated. The agglutinability of ginsenoside-Rc was effective for phosphatidylcholines with short or unsaturated fatty acyl chains. The results suggested that the interaction of ginsenoside-Rc with phospholipid membranes should be affected not only by the chemical structure of the phospholipid but also by the membrane fluidity. 相似文献
173.
Kimiko Tatsumi Shoji Tsuji Hideki Miwa Mutsuo Nuriya Kazunari Kaneko Masato Yasui 《FEBS letters》2009,583(12):2077-2082
The neurogenic gene Drosophilabig brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay using L cells. Bib-transfected L cells formed aggregated clusters, while control-L cells remained as a single cell suspension. Ion permeation was not confirmed in L cells stably expressing Bib. When expressed in COS7 cells, Bib exhibited limited water permeability. This newly found cell adhesive function of Bib may be important for Drosophila neurogenesis. 相似文献
174.
175.
Koki Kuramochi Kohsuke Uchimura Atsushi Kurata Tohru Kobayashi Yuu Hirose Takeshi Miura Noriaki Kishimoto Ron Usami Koki Horikoshi 《Extremophiles : life under extreme conditions》2016,20(4):471-478
An endo β-1,4-xylanase (XynE15) from a culture broth of a deep subseafloor microorganism, Microcella alkaliphila JAM-AC0309, was purified to homogeneity. The molecular mass of XynE15 was approximately 150 kDa as judged by SDS-PAGE. The optimal pH and temperature for hydrolysis of xylan were pH 8 and 65 °C. The enzyme was stable to incubation for 30 min at up to 75 °C, and the half-life at 50 °C was 48 h. XynE15 hydrolyzed arabinoxylan, oat spelt xylan, and birchwood xylan well, but not avicel, carboxymethylcellulose, or arabinan. Xylooligosaccharides were hydrolyzed to mainly xylobiose from higher than xylotetraose. The genome sequencing analysis of strain JAM-AC03039 revealed that XynE15 was composed of 1,319 amino acids with one catalytic domain and three carbohydrate-binding domains belonging to glycoside hydrolase (GH) family 10 and carbohydrate-binding module (CBM) family 4, respectively. 相似文献
176.
Madoka E. Hazemi Kazumitsu Onizuka Tomohito Kobayashi Akira Usami Norihiro Sato Fumi Nagatsugi 《Bioorganic & medicinal chemistry》2018,26(12):3551-3558
Higher-order structures of nucleic acids have become widely noted for their biological consequences and the discovery of an alkylating small molecule for these structures has been of interest due to its therapeutic potential. We previously developed the vinyldiaminotriazine (VDAT)-acridine conjugate as a T-T mismatch alkylating agent. In this report, we focused on the finding of the alkylation to the G-quadruplex (G4) DNA with VDAT-acridine conjugates. The VDAT-acridine conjugates exhibited a considerable alkylation ability to G4 under mild conditions. Moreover, the investigation of properties with the alkylated G4 revealed that alkylation by this conjugate significantly increased the stability of the G4 structure. This study provides a starting point in the further development of selective G4 alkylating small molecules. 相似文献
177.
178.
179.
The gobiid fish Trimma okinawae changes its sex bi-directionally according to its social status. Morphological changes in the urinogenital papillae (UGP) of this fish have been reported during sex change. However, there have been no detailed observations of such changes. Here, we histologically examined the UGP structure of male- and female-phase fish. UGPs of fish in female and male phase contained both oviducts and sperm ducts. Both ducts were coalesced into one duct within the posterior region of the UGP. Female-phase fish had many longitudinal folds in the hypertrophied tunica mucosa of the oviduct, which was found to be responsible for the transport of eggs and the removal of follicular cells from the oocyte. In contrast, male-phase fish had an immature oviduct and a mature sperm duct in the UGP. In the male-phase fish, the co-existence of spermatozoa and fibrillar secretions was observed in the sperm duct during spermiation. 相似文献
180.
Junichi Nasu Kyoko Murakami Shoji Miyagawa Ryosuke Yamashita Tohru Ichimura Takaji Wakita Hak Hotta Tatsuo Miyamura Tetsuro Suzuki Tazuko Satoh Ikuo Shoji MD PhD 《Journal of cellular biochemistry》2010,111(3):676-685
E6‐associated protein (E6AP) is a cellular ubiquitin protein ligase that mediates ubiquitylation and degradation of tumor suppressor p53 in conjunction with the high‐risk human papillomavirus E6 protein. We previously reported that E6AP targets annexin A1 protein for ubiquitin‐dependent proteasomal degradation. To gain a better understanding of the physiological function of E6AP, we have been seeking to identify novel substrates of E6AP. Here, we identified peroxiredoxin 1 (Prx1) as a novel E6AP‐binding protein using a tandem affinity purification procedure coupled with mass spectrometry. Prx1 is a 25‐kDa member of the Prx family, a ubiquitous family of antioxidant peroxidases that regulate many cellular processes through intracellular oxidative signal transduction pathways. Immunoprecipitation analysis showed that E6AP binds Prx1 in vivo. Pull‐down experiments showed that E6AP binds Prx1 in vitro. Ectopic expression of E6AP enhanced the degradation of Prx1 in vivo. In vivo and in vitro ubiquitylation assays revealed that E6AP promoted polyubiquitylation of Prx1. RNAi‐mediated downregulation of endogenous E6AP increased the level of endogenous Prx1 protein. Taken together, our data suggest that E6AP mediates the ubiquitin‐dependent proteasomal degradation of Prx1. Our findings raise a possibility that E6AP may play a role in regulating Prx1‐dependent intracellular oxidative signal transduction pathways. J. Cell. Biochem. 111: 676–685, 2010. © 2010 Wiley‐Liss, Inc. 相似文献