排序方式: 共有62条查询结果,搜索用时 6 毫秒
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L V Ponomareva I P Peshkova N P Tsvetkova V I Iakovlev V G Shmeleva V G Krunchak N P Shchipanov 《Antibiotiki i khimioterapii͡a》1990,35(4):42-45
The process of enzymatic hydrolysis of the mycelial waste from the manufacture of tetracycline with using Streptomyces aureofaciens was studied. For the enzymatic hydrolysis, neutral and alkaline proteinases were used. It was shown that alkaline proteinase (protosubtilin G10X) provided the most efficient hydrolysis. Optimal conditions for the hydrolysis were determined: a temperature of 42 degrees C, hydrolysis time of 4 to 6 hours and enzyme concentrations of 1.25 to 2.20 mg/ml at a mycecial waste concentration of 12.5 mg/ml. The time course of changes in amino acid and amine nitrogen levels during enzymatic hydrolysis was investigated. It was demonstrated that the hydrolysis efficiency depended on the mode of enzyme addition. The highest efficiency was observed with fractional feeding of the enzyme. 相似文献
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I S Breslav G G Isaev A M Shmeleva 《Biulleten' eksperimental'no? biologii i meditsiny》1976,82(11):1283-1285
Dynamics of pulmonary ventilation, electric activity of the intercostal muscles and of the alveolar gas composition was studied in 12 healthy men during dosaged muscular work; these men were given different gas mixtures to breathe. The respiratory response at the initial period of work in inhalation of the hypoxic-hypercapnic gas mixture was greater than that in persons who breathed room air. This response practically disappeared after oxygen hyperventilation. Apparently the rapid component of the ventilation response to the muscular work was largely due to increased sensitivity of the respiratory centre to the chemoreceptive drive. 相似文献
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Preparation and properties of des-(pentapeptide-B26-30)-insulin 总被引:2,自引:0,他引:2
Iu P Shvachkin G A Shmeleva V F Krivtsov V P Fedotov A I Ivanova 《Biokhimii?a (Moscow, Russia)》1972,37(5):966-973
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G M Bolgareva E K Krivtsova Iu A Revazova N S Zakharova I G Bazhanova T N Remova E I Shmeleva N U Mertsalova E E Sukhinova E S Revazova 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1992,(7-8):42-45
Two vaccine preparations obtained from Bordetella pertussis, whole-cell vaccine constituting one of the components of adsorbed DPT vaccine and acellular vaccine, were tested for mutagenicity. The doses of the preparations covered the range 1-100 ED50. Ames' test and the metaphase analysis of marrow cells of C57BL/6J mice were used. The acellular preparation was also tested on thymectomized mice, taking into consideration chromosomal aberrations in marrow metaphases. Whole-cell and acellular pertussis vaccines did not induce mutations in Salmonella typhimurium and chromosomal aberrations in mouse marrow cells. 相似文献
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M I Iankevich V G Shmeleva V I Iakovlev L V Ponomareva 《Prikladnaia biokhimiia i mikrobiologiia》1981,17(4):500-504
Trypsin immobilization in an inorganic polymer, i.e. silica gel, was investigated. Properties of the immobilized enzyme were examined. It was found that the enzyme retained its activity, depending on the gel genesis and dehydration of the carrier. The effect of stabilizing supplements on the enzyme activity was studied. The enzyme immobilization was accompanied by an increase in its thermostability. 相似文献
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