Complete Genome Sequence of Hepatitis E Virus from Rabbits in the United States

Hepatitis E virus (HEV) is a single-strand positive-sense RNA virus in the family Hepeviridae. The disease caused by HEV, hepatitis E, is an important public health problem in developing countries of Asia and Africa and is also endemic in many industrialized countries, including the United States. HEV has been identified from several other animal species in addition to humans, including the pig, chicken, mongoose, deer, rabbit, ferret, bat, and fish. Here we report the complete genome sequence of the first strain of HEV from rabbits in the United States. Sequence and phylogenetic analyses revealed that the U.S. rabbit HEV is a distant member of the zoonotic genotype 3 HEV, thus raising a concern for potential zoonotic human infection. A unique 90-nucleotide insertion within the X domain of the ORF1 was identified in the rabbit HEV, and this insertion may play a role in the species tropism of HEV.     

高等植物叶片中的硝酸还原酶活性稳定因子

从小麦、油菜、浮萍、番茄、烟草的叶片中分离得到NR-SF。不同植物材料中NR及NR-SF能起交叉反应;不同NR-SF影响NR酶动力学性质相同;不同NR-SF的凝胶电泳谱带显示蛋白和糖蛋白性质。NR-SF广泛存在于植物细胞中。     

土壤中含EB病毒诱导物的检测

从广西壮族自治区梧州市、苍梧县、罗城县和北京市收集的土壤标本中发现有EB病毒诱导物。梧州市和苍梧县沿公路和江河两旁桐油树下的土壤标本,对EB病毒早期抗原诱导的阳性率为40～58％。在其他大戟科植物下的土壤标本中,也发现有EB病毒诱导物。对桐油树下土壤中EB病毒诱导物与鼻咽癌发生的可能关系进行了讨论。     

水稻、小麦、油菜和烟草细胞质雄性不育系统中组分Ⅰ蛋白的比较分析

组分Ⅰ蛋白(RuBP羧化酶/加氧酶)的生物合成系由叶绿体基因和细胞核基因共同控制,所以,被作为研究细胞质遗传的标记。本实验用免疫化学和氨基酸成分分析等方法,对水稻(珍汕97)、小麦(繁7)、油菜(湘矮早)和烟草(G28)的细胞质雄性不育系及其保持系的组分Ⅰ蛋白作了比较,同时对不同作物的组分Ⅰ蛋白也作了免疫鉴定。结果表明,细胞质雄性不育系及其保持系的组分Ⅰ蛋白差异不大,但是,四种不同作物的组分Ⅰ蛋白之间有明显差异。     

Two temporally synthesized charge subunits interact to form the five isoforms of cottonseed (Gossypium hirsutum) catalase.

Five charge isoforms of tetrameric catalase were isolated from cotyledons of germinated cotton (Gossypium hirsutum L.) seedlings. Denaturing isoelectric focusing of the individual isoforms in polyacrylamide gels indicated that isoforms A (most anodic) and E (most cathodic) consisted of one subunit of different charge, whereas isoforms B, C and D each consisted of a mixture of these two subunits. Thus the five isoforms apparently were formed through combinations of two subunits in different ratios. Labelling cotyledons in vivo with [35S]methionine at three daily intervals in the dark, and translation in vivo of polyadenylated RNA isolated from cotyledons at the same ages, revealed synthesis of two different subunits. One of the subunits was synthesized in cotyledons at all ages studied (days 1-3), whereas the other subunit was detected only at days 2 and 3. This differential expression of two catalase subunits helped explain previous results from this laboratory showing that the two anodic forms (A and B) found in maturing seeds were supplemented with three cathodic forms (C-E) after the seeds germinated. These subunit data also helped clarify our new findings that proteins of isoforms A, B and C (most active isoforms) accumulated in cotyledons of plants kept in the dark for 3 days, then gradually disappeared during the next several days, whereas isoforms D and E (least active isoforms) remained in the cells. This shift in isoform pattern occurred whether seedlings were kept in the dark or exposed to continuous light after day 3, although exposure to light enhanced this process. These sequential molecular events were responsible for the characteristic developmental changes (rise and fall) in total catalase activity. We believe that the isoform changeover is physiologically related to the changeover in glyoxysome to leaf-type-peroxisome metabolism.     

闽北地区四代三化螟发生动态Fuzzy预测模型研究

根据Fuzzy数学原理和三化螟生物学特性,组建了四代三化螟发生动态综合预测模型经对福建省将乐县16年四代三化螟发生期、发生量回报及1995年的预报,拟合率及正确率均达100％。     

钆和镱对人红细胞膜脂及膜蛋白的作用

利用荧光偏振,自旋标标顺磁共振波谱和激光拉曼技术研究了钆和镱对人红细胞膜结构和功能的影响。结果表明,低浓度的Ｇｄ３＋（０．５μｍｏｌ／Ｌ）对（Ｎａ＋＋Ｋ＋）－ＡＴＰ酶和Ｍｇ２＋－ＡＴＰ酶有轻微的激活作用,而随着其浓度的增大,则明显抑制酶的活性,Ｇｄ３＋与Ｙｂ３＋和人红细胞膜作用后,降低膜脂流动性,并使膜蛋白酰胺Ｉ＇－α螺旋振动强度减弱．     

油菜菌核病菌生理生态研究

通过试验分析,结果得出,油菜菌核病菌生长温度范围在0～34℃之间,23℃以下菌丝生长速度与温度呈正相关,25℃以上则呈负相关,23～25℃最适宜,菌丝和菌核致死温度分别为45℃和75℃．该菌对酸碱度适应范围很广,PH1～14范围内只在PH_1条件下不能生长,其最适PH值为5～6。病菌利用铵态氮源能力最强,硝态氮源次之,不能利用亚硝态氮;对氨基酸利用能力以精氨酸最强,其次是酪氨酸、天门冬氨酸和谷氨酸,赖氨酸最差。植株内精氨酸和赖氨酸比值大的白菜型油菜“黄鳝籽”病情扩展最快。