排序方式: 共有219条查询结果,搜索用时 15 毫秒
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Hayley E. Bugeja Kylie J. Boyce Harshini Weerasinghe Sally Beard Anne Jeziorowski Shivani Pasricha Michael Payne Lena Schreider Alex Andrianopoulos 《Fungal genetics and biology : FG & B》2012,49(10):772-778
Penicillium marneffei is an opportunistic pathogen of humans and displays a temperature dependent dimorphic transition. Like many fungi, exogenous DNA introduced by DNA mediated transformation is integrated randomly into the genome resulting in inefficient gene deletion and position-specific effects. To enhance successful gene targeting, the consequences of perturbing components of the non-homologous end joining recombination pathway have been examined. The deletion of the KU70 and LIG4 orthologs, pkuA and ligD, respectively, dramatically enhanced the observed homologous recombination frequency leading to efficient gene deletion. While ΔpkuA was associated with reduced genetic stability over-time, ΔligD represents a suitable recipient strain for downstream applications and combined with a modified Gateway? system for the rapid generation of gene deletion constructs, this represents an efficient pipeline for characterizing gene function in P. marneffei. 相似文献
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Drebrin A, an actin-binding protein, is a key regulatory element in synaptic plasticity of neuronal dendrites. Understanding how drebrin binds and remodels F-actin is important for a functional analysis of their interactions. Conventionally, molecular models for protein-protein interactions use binding parameters derived from bulk solution measurements with limited spatial resolution, and the inherent assumption of homogeneous binding sites. In the case of actin filaments, their structural and dynamic states—as well as local changes in those states—may influence their binding parameters and interaction cooperativity. Here, we probed the structural remodeling of single actin filaments and the binding cooperativity of DrebrinA1-300 –F–actin using AFM imaging. We show direct evidence of DrebrinA1-300-induced cooperative changes in the helical structure of F-actin and observe the binding cooperativity of drebrin to F-actin with nanometer resolution. The data confirm at the in vitro molecular level that variations in the F-actin helical structure can be modulated by cooperative binding of actin-binding proteins. 相似文献
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Bhardwaj Shivani Pathak Nilesh Kumar Ji Alok Uma R. Sharma R. P. 《Plasmonics (Norwell, Mass.)》2017,12(1):193-201
Plasmonics - Graphene plasmonic resonances play a significant role for enhancing the photon absorption inside thin film solar devices. We investigate the field rising at the intersection of... 相似文献
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Distinct roles of the 7‐transmembrane receptor protein Rta3 in regulating the asymmetric distribution of phosphatidylcholine across the plasma membrane and biofilm formation in Candida albicans 下载免费PDF全文
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Sayan Chattopadhyay Rachita Arora Amit Kishor Shivani Singh Abhijeet Alok Mainak Mitra 《Bioinformation》2022,18(3):165
Mucin 4 (MUC4) and matrix metalloproteinase 7 (MMP7) have been reported to be associated with chronic periodontitis as seen in gingival tissue biopsies. Therefore, it is of interest to estimate the levels of MUC4 and MMP7 in saliva and gingival crevicular fluid (GCF) samples of periodontitis in adolescents patients at West Bengal, India. MUC4 levels were significantly lower in saliva and GCF from periodontitis patients compared to healthy controls. However, MMP7 levels were found to be significantly higher in saliva and GCF from periodontitis patients. Thus, MUC4 and MMP7 are biomarkers for periodontitis diagnosis and towards further consideration. 相似文献