Author Keywords: CO2; demand-controlled ventilation systems; IAQ 相似文献
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Background
Optimization of the differentiation medium through using autologous factors such as PRP is of great consideration, but due to the complex, variable and undefined composition of PRP on one hand and lack of control over the absolute regulatory mechanisms in in vitro conditions or disrupted and different mechanisms in diseased tissue microenvironments in in vivo conditions on the other hand, it is complicated and rather unpredictable to get the desired effects of PRP making it inevitable to monitor the possible pathologic or undesired differentiation pathways and therapeutic effects of PRP. Therefore, in this study the probable potential of PRP on inducing calcification, inflammation and angiogenesis in chondrogenically-differentiated cells was investigated.Methods
The expressions of chondrogenic, inflammatory, osteogenic and angiogenic markers from TGFβ or PRP-treated cells during chondrogenic differentiation of human adipose-derived stem cells (ADSCs) was evaluated. Expressions of Collagen II (Col II), Aggrecan, Sox9 and Runx2 were quantified using q-RT PCR. Expression of Col II and X was investigated by immunocytochemistry as well. Glycosaminoglycans (GAGs) production was also determined by GAG assay. Possible angiogenic/inflammatory potential was determined by quantitatively measuring the secreted VEGF, TNFα and phosphorylated VEGFR2 via ELISA. In addition, the calcification of the construct was monitored by measuring ALP activity and calcium deposition.Results
Our data showed that PRP positively induced chondrogenesis; meanwhile the secretion of angiogenic and inflammatory markers was decreased. VEGFR2 phosphorylation and ALP activity had a decreasing trend, but tissue mineralization was enhanced upon treating with PRP.Conclusions
Although reduction in inflammatory/angiogenic potential of the chondrogenically differentiated constructs highlights the superior effectiveness of PRP in comparison to TGFβ for chondrogenic differentiation, yet further improvement of the PRP-based chondrogenic differentiation media is required to inhibit the production of angiogenic/inflammatory markers, calcification and the release of synthesized GAG out of the construct. 相似文献1. 1. The feasibility of controlling the ventilation system using the occupant-generated carbon dioxide as an indicator of ventilation rate and indoor air quality has been investigated in an eleven storey office building.
2. 2. The study compares the indoor environment created by two different types of ventilation control systems.
3. 3. The two ventilation systems tested consisted of: a conventional system controlled by outdoor temperatures, and a demand-controlled system regulated by indoor carbon dioxide concentration.
4. 4. The results show that the CO2-based demand-controlled ventilation system does not worsen indoor air quality and thermal comfort. It was also noticed that an energy saving of 12% was achieved using the CO2 control system.
5. 5. The occupants perceived that their productivity is proportional to their perception of the indoor environment; indicating that higher productivity rates can be achieved by better controlling the working environment above satisfactory levels.