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131.
Elisa Bellucci Orlando Mario Aguilar Saleh Alseekh Kirstin Bett Creola Brezeanu Douglas Cook Lucía De la Rosa Massimo Delledonne Denise F. Dostatny Juan J. Ferreira Valérie Geffroy Sofia Ghitarrini Magdalena Kroc Shiv Kumar Agrawal Giuseppina Logozzo Mario Marino Tristan Mary-Huard Phil McClean Vladimir Meglič Tamara Messer Frédéric Muel Laura Nanni Kerstin Neumann Filippo Servalli Silvia Străjeru Rajeev K. Varshney Marta W. Vasconcelos Massimo Zaccardelli Aleksei Zavarzin Elena Bitocchi Emanuele Frontoni Alisdair R. Fernie Tania Gioia Andreas Graner Luis Guasch Lena Prochnow Markus Oppermann Karolina Susek Maud Tenaillon Roberto Papa 《The Plant journal : for cell and molecular biology》2021,108(3):646-660
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Jitendra Kumar Ekta Srivastva Mritunjay Singh Shiv Kumar N. Nadarajan Ashutosh Sarker 《Physiology and Molecular Biology of Plants》2014,20(1):125-132
Genetic diversity was studied among 21 accessions of lentil using SSR markers and morphological traits in order to assess the diversification of Indian gene-pool of lentil through introgression of exotic genes and introduction of germplasm. Among these , 16 genotypes either had ‘Precoz’ gene, an Argentine line in their pedigree or genes from introduced lines from ICARDA. Sixty five SSR markers and eight phenotypic traits were used to analyse the level of genetic diversity in these genotypes. Forty three SSR markers (66 %) were polymorphic and generated a total of 177 alleles with an average of 4.1 alleles per SSR marker. Alleles per marker ranged from 2 to 6. The polymorphic information content ranged 0.33 to 0.80 with an average of 0.57, suggesting that SSR markers are highly polymorphic among the studied genotypes. Genetic dissimilarity based a dendrogram grouped these accessions into two main clusters (cluster I and cluster II) and it ranged 33 % to 71 %, suggesting high level of genetic diversity among the genotypes. First three components of PCA based morphological traits explained higher variance (95.6 %) compared to PCA components based on SSR markers (42.7 %) of total genetic variance. Thus, more diversity was observed for morphological traits and genotypes in each cluster and sub-cluster showed a range of variability for seed size, earliness, pods/plant and plant height. Molecular and phenotypic diversity analysis thus suggested that use of germplasm of exotic lines have diversified the genetic base of lentil germplasm in India. This diversified gene-pool will be very useful in the development of improved varieties of lentil in order to address the effect of climate change, to adapt in new cropping systems niches such as mixed cropping, relay cropping, etc. and to meet consumers’ preference. 相似文献
135.
Shiv Poojan Anupam Dhasmana Qazi Mohammad Sajid Jamal Mohd Haneef Mohtashim Lohani 《Bioinformation》2014,10(8):474-479
Arsenic is the most toxic metalloid present in the natural environment in both organic and inorganic arsenic forms. Inorganic
arsenic is often more hazardous than the organic form. Arsenite and arsenate compounds are the major inorganic forms which are
toxic causing severe human health dysfunction including cancer. Excretion of arsenic from the system is found elusive. Therefore, it
is of interest to screen channel proteins with the arsenic complex in the different combination of arsenic, GSH (glutathione) and
arsenic, selenium using docking methods. The mode of arsenic removal. The complex structure revealed the mode of arsenic
binding efficiency with the receptor aquaporine 9 and ABCC1 channel protein. This provides insights to understand the
mechanism of arsenic efflux. These inferences find application in the design, identification and development of novel nutracetucal
or any other formulation useful in the balance of arsenic efflux. 相似文献
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Purnima Singh Shiv M. Singh Prashant Dhakephalkar 《Extremophiles : life under extreme conditions》2014,18(2):229-242
Cryoconite holes have biogeochemical, ecological and biotechnological importance. This communication presents results on culturable psychrophilic bacterial diversity from cryoconite holes at Midre Lovénbreen (ML), Austre Brøggerbreen (AB), and Vestre Brøggerbreen (VB) glaciers. The culturable bacterial count ranged from 2.7 × 103 to 8.8 × 104 CFUs/g while the total bacterial numbers ranged from 5.07 × 105 to 1.50 × 106 cells at the three glaciers. A total of 35 morphologically distinct bacterial isolates were isolated. Based on 16S rRNA gene sequence data, the identified species belonged to eight genera namely Pseudomonas, Polaromonas, Micrococcus, Subtercola, Agreia, Leifsonia, Cryobacterium and Flavobacterium. The isolates varied in their growth temperature, NaCl tolerance, growth pH, enzyme activities, carbon utilization and antibiotic sensitivity tests. Fatty acid profiles indicate the predominance of branched fatty acids in the isolates. To the best of our knowledge, this is the first record of culturable bacterial communities and their characterization from glacier cryoconites from High Arctic. High amylase and protease activities expressed by Micrococcus sp. MLB-41 and amylase, protease and lipase activities expressed by Cryobacterium sp. MLB-32 provide a clue to the potential applications of these organisms. These cold-adapted enzymes may provide an opportunity for the prospect of biotechnology in Arctic. 相似文献
138.
Niranjan Mishra Katherukamem Rajukumar Shruti Shrikant Pitale Anil Prakash Ram Kumar Nema Sthita Pragnya Behera Shiv Chandra Dubey 《Journal of biosciences》2010,35(1):79-86
Bovine viral diarrhoea virus (BVDV) is an economically important pathogen of cattle and sheep belonging to the genus Pestivirus of the family Flaviviridae. Although the BVDV non-structural N-terminal protease (Npro) acts as an interferon antagonist and subverts the host innate immunity, little is known about its immunogenicity. Hence,
we expressed a recombinant BVDV Npro-His fusion protein (28 kDa) in E. coli and determined the humoral immune response generated by it in rabbits. The antigenicity of the Npro protein was confirmed by western blot using anti-BVDV hyperimmune cattle, sheep and goat serum, and anti-Npro rabbit serum. When rabbits were immunized with the Npro protein, a humoral immune response was evident by 4 weeks and persisted till 10 weeks post immunization as detected by ELISA
and western blot. Despite Npro-specific antibodies remaining undetectable in 80 serum samples from BVDV-infected sheep and goats, BVDV hyperimmune sera
along with some of the field cattle, sheep and goat sera with high BVDV neutralizing antibody titres were found positive for
Npro antibodies. Our results provide evidence that despite the low immunogenicity of the BVDV Npro protein, a humoral immune response is induced in cattle, sheep and goats only with repeated BVDV exposure. 相似文献
139.
Phenotypic characterization of telomerase-immortalized primary non-malignant and malignant tumor-derived human prostate epithelial cell lines 总被引:5,自引:0,他引:5
Gu Y Li H Miki J Kim KH Furusato B Sesterhenn IA Chu WS McLeod DG Srivastava S Ewing CM Isaacs WB Rhim JS 《Experimental cell research》2006,312(6):831-843
In vitro human prostate cell culture models are critical for clarifying the mechanism of prostate cancer progression and for testing preventive and therapeutic agents. Cell lines ideal for the study of human primary prostate tumors would be those derived from spontaneously immortalized tumor cells; unfortunately, explanted primary prostate cells survive only short-term in culture, and rarely immortalize spontaneously. Therefore, we recently have generated five immortal human prostate epithelial cell cultures derived from both the benign and malignant tissues of prostate cancer patients with telomerase, a gene that prevents cellular senescence. Examination of these cell lines for their morphologies and proliferative capacities, their abilities to grow in low serum, to respond to androgen stimulation, to grow above the agar layer, to form tumors in SCID mice, suggests that they may serve as valid, useful tools for the elucidation of early events in prostate tumorigenesis. Furthermore, the chromosome alterations observed in these immortalized cell lines expressing aspects of the malignant phenotypes imply that these cell lines accurately recapitulate the genetic composition of primary tumors. These novel in vitro models may offer unique models for the study of prostate carcinogenesis and also provide the means for testing both chemopreventive and chemotherapeutic agents. 相似文献
140.