Corticosterone reception by alveolar macrophages when their functional activity has changed

The binding of 3H-corticosterone by rat alveolar macrophages was studied before and after stimulation with zymosan in vivo. Thirty min after incubation of the macrophagal monolayer from intact animals with 3H-corticosterone accumulation of the hormone by the cells came to an end. As the concentration of 3H-corticosterone in the incubation medium was raised, the binding of the hormone with the saturated (receptor) system of alveolar macrophages terminated upon absorption of 10.6 fmol per 10(6) cells. Further raising of the level of the bound hormone was effected by the unsaturated (lipid) system. Stimulation with zymosan led not only to an increase in the number of the cells of the bronchoalveolar tract but also to an elevation of the intensity of 3H-corticosterone engulfment by alveolar macrophages. The number of binding sites per cell in the zymosan-activated macrophages increased 1.5-fold. This may be an important moment determining the development and liquidation of mononuclear infiltrations in the lung.     

Changes in Gene Expression and Neuroinflammation in the Hippocampus after Focal Brain Ischemia: Involvement in the Long-Term Cognitive and Mental Disorders

Biochemistry (Moscow) - Ischemic brain injuries are accompanied by the long-term changes in gene expression in the hippocampus, the limbic system structure, involved in the regulation of key...     

Genetic correlation between the arterial pressure response during emotional stress and the alpha1-adrenoreceptor concentration in brain regions]

Arterial blood pressure reactivity to the emotional stress and brain alpha 1-adrenoreceptors concentrations were studied in hypertensive (ISIAH strain) and normotensive (Wistar strain) rats and their F1 and F2 hybrids. Significant correlations between the stress-induced increase in the arterial blood pressure and the amount of alpha 1-adrenoreceptors in hypothalamus (+0.46) and medulla (+0.38) were found in the F2. This cosegregation may point to the significant role of genetically determined peculiarities of expression of alpha 1-adrenoreceptors in brain regions during pathogenesis of arterial hypertension in the ISIAH strain.     

An approach to isolating individual tRNA from the human placenta by affinity chromatography. Isolation of highly purified Phe-tRNA Phe

A new approach to isolation of individual tRNAs from eukaryotes based on affinity chromatography is suggested. At first, using a sorbent with oligonucleotide pTGGT attached, the total tRNA with native CCA-ends was obtained. Then by means of a sorbent with oligonucleotide pTTCAG immobilized, which is complementary to a part of the tRNA(Phe) anticodon loop, tRNA(Phe) with the acceptor activity greater than 1000 pmole/unit was isolated.     

Studies on phosphate metabolism in obligate methanotrophs

Abstract Studies on three representative species of methane-utilizing bacteria revealed high intracellular levels of inorganic pyrophosphate (5 mM) in contrast to low levels of ATP (0.5 mM) as well as the differences in the sets of enzymes utilizing pyro- and polyphosphates as phosphate and metabolic energy donors.