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61.
62.
Onodera H Kaneko M Takahashi Y Uochi Y Funahashi J Nakashima T Soga S Suzuki M Ikeda S Yamashita Y Rahayu ES Kanda Y Ichimura M 《Bioorganic & medicinal chemistry letters》2008,18(5):1588-1591
Hsp90 is an attractive chemotherapeutic target because it is essential to maturation of multiple oncogenes. We describe the conformational significance of EH21A1-A4, phenolic derivatives of geldanamycin isolated from Streptomyces sp. Their native free structures are similar to the active form of geldanamycin bound to Hsp90 protein. Their conformational character is a probable reason for their high-affinity binding. Lack of toxic benzoquinone in EH21A1-A4 also adds to their potential as lead compounds for anti-tumor drugs. 相似文献
63.
Akira Watanabe Shiro Yamaguchi Koichiro Urabe Yasuhiko Asada 《Journal of Molecular Catalysis .B, Enzymatic》2003,23(2-6):379
Free
-alanine was detected in a cell extract of the fruit-body of an edible basidiomycetous mushroom, Lentinus edodes (Shiitake), by means of reverse-phase high performance liquid chromatography. We also found an amino acid racemase activity in L. edodes fruit-body, and purified the enzyme. The enzyme has a molecular weight of approximately 86,000, and consists of two subunits of identical molecular weight (44,000). The optimal pH of the enzyme activity is around pH 9.5 for both
-to-
and
-to-
alanine racemization. The enzyme requires pyridoxal 5′-phosphate as a cofactor. Km and Vmax values for
-alanine were 37.3 mM and 520 nmol/min/mg, respectively; for
-alanine, they were 9.21 mM and 141 nmol/min/mg, respectively. The equilibrium constant was calculated to be 1.10, which is consistent with the theoretical value for the racemase reaction. The ability of the enzyme to catalyze the racemization of various
-amino acids was investigated. The enzyme catalyzes the racemization of
-serine (relative reaction rate, 144% of rate for
-alanine),
-alanine (100%),
-homoserine (17.1%),
-2-aminobutyrate (5.6%),
-glutamate (4.5%), and
-asparagine (3.2%). To the best of our knowledge, this is the first report of an amino acid racemase produced by a basidiomycetous mushroom. 相似文献
64.
Downregulation of p‐COUMAROYL ESTER 3‐HYDROXYLASE in rice leads to altered cell wall structures and improves biomass saccharification 下载免费PDF全文
Yuri Takeda Yuki Tobimatsu Steven D. Karlen Taichi Koshiba Shiro Suzuki Masaomi Yamamura Shinya Murakami Mai Mukai Takefumi Hattori Keishi Osakabe John Ralph Masahiro Sakamoto Toshiaki Umezawa 《The Plant journal : for cell and molecular biology》2018,95(5):796-811
p‐Coumaroyl ester 3‐hydroxylase (C3′H) is a key enzyme involved in the biosynthesis of lignin, a phenylpropanoid polymer that is the major constituent of secondary cell walls in vascular plants. Although the crucial role of C3′H in lignification and its manipulation to upgrade lignocellulose have been investigated in eudicots, limited information is available in monocotyledonous grass species, despite their potential as biomass feedstocks. Here we address the pronounced impacts of C3′H deficiency on the structure and properties of grass cell walls. C3′H‐knockdown lines generated via RNA interference (RNAi)‐mediated gene silencing, with about 0.5% of the residual expression levels, reached maturity and set seeds. In contrast, C3′H‐knockout rice mutants generated via CRISPR/Cas9‐mediated mutagenesis were severely dwarfed and sterile. Cell wall analysis of the mature C3′H‐knockdown RNAi lines revealed that their lignins were largely enriched in p‐hydroxyphenyl (H) units while being substantially reduced in the normally dominant guaiacyl (G) and syringyl (S) units. Interestingly, however, the enrichment of H units was limited to within the non‐acylated lignin units, with grass‐specific γ‐p‐coumaroylated lignin units remaining apparently unchanged. Suppression of C3′H also resulted in relative augmentation in tricin residues in lignin as well as a substantial reduction in wall cross‐linking ferulates. Collectively, our data demonstrate that C3′H expression is an important determinant not only of lignin content and composition but also of the degree of cell wall cross‐linking. We also demonstrated that C3′H‐suppressed rice displays enhanced biomass saccharification. 相似文献
65.
Shiro Higashi Akinori Nakashima Hideki Ozaki Mikiko Abe Toshiki Uchiumi 《Journal of plant research》1993,106(1):47-54
The process of digestion of captured feeds in a pitcher, an insect-trapping organ, ofNepenthes was studied. Changes in bacterial population, pH and NH4
+ concentrations in pitcher juice were examined. Strong activities of both acid- and alkaline phosphatase, phosphoamidase,
esterase C4 and esterase C8 were found in the pitcher juice. Optimum pH of proteases in the juice and those secreted from
bacteria showed pH 3.0 and pH 8.0–9.0, respectively. Twenty six strains of bacteria were isolated from 4 pitchers: 10 strains
were gram positive, 16 strains were gram negative (10 strains had casein hydrolase activity). A proton excretion was induced
by NH4
+ released from the added solutions, and accordingly, the pH of the solutions fell. As a simulation model of the digestion
process of feeds in pitcher juice and polypeptone solution was added into the washed pitcher. A good correlation was found
among the NH4
+ concentration, pH and bacterial cell titer. 相似文献
66.
Watanabe S Nagashio Y Asaumi H Nomiyama Y Taguchi M Tashiro M Kihara Y Nakamura H Otsuki M 《American journal of physiology. Gastrointestinal and liver physiology》2004,287(6):G1175-G1181
Pancreatic stellate cells (PSCs) play a central role in development of pancreatic fibrosis. In chronic pancreatitis, pancreatic tissue pressure is higher than that of the normal pancreas. We here evaluate the effects of pressure on the activation of rat PSCs. PSCs were isolated from the pancreas of Wistar rat using collagenase digestion and centrifugation with Nycodenz gradient. Pressure was applied to cultured rat PSCs by adding compressed helium gas into the pressure-loading apparatus to raise the internal pressure. Cell proliferation rate was assessed by 5-bromo-2'-deoxyuridine (BrdU) incorporation. MAPK protein levels and alpha-smooth muscle actin (alpha-SMA) expression were evaluated by Western blot analysis. Concentration of activated transforming growth factor-beta1 (TGF-beta1) secreted from PSCs into culture medium was determined by ELISA. Collagen type I mRNA expression and collagen secretion were assessed by quantitative PCR and Sirius red dye binding assay, respectively. Application of pressure significantly increased BrdU incorporation and alpha-SMA expression. In addition, pressure rapidly increased the phosphorylation of p44/42 and p38 MAPK. Treatment of PSCs with an MEK inhibitor and p38 MAPK inhibitor suppressed pressure-induced cell proliferation and alpha-SMA expression, respectively. Moreover, pressure significantly promoted activated TGF-beta1 secretion, collagen type I mRNA expression, and collagen secretion. Our results demonstrate that pressure itself activates rat PSCs and suggest that increased pancreatic tissue pressure may accelerate the development of pancreatic fibrosis in chronic pancreatitis. 相似文献
67.
68.
The effect of cyanide on ammonia and urea metabolism was studiedwith intact cells of Chlorella ellipsoidea Gerneck, a greenalga which apparently lacks urease. Ammonia uptake was inhibited more readily by cyanide than wasurea uptake. Urea uptake was stimulated by lower concentrationsof cyanide. The addition of cyanide caused the formation ofammonia from some cellular nitrogenous compounds. In the presenceof exogenously added urea, the molar ratio of ammonia accumulatedin the medium to urea taken up exceeded 2.0 as the cyanide concentrationincreased. However, the molar ratio of ammonia actually producedfrom urea nitrogen to urea taken up was less than 1.35 at anyconcentration of cyanide tested. In the presence of higher concentrationsof cyanide, the rate of incorporation of 15N into amino acidsfrom 15N-urea was higher than that from 15N-ammonium sulfate. The results suggest that Chlorella ellipsoidea possesses a pathwaythrough which urea nitrogen is assimilated directly withouta preliminary breakdown to ammonia. (Received October 18, 1976; ) 相似文献
69.
Summary Using an ethanol solution of nile blue, we have developed an efficient method to detect the colonies of poly(3-hydroxyalkanoic
acids) (PHA) producing bacteria on the agar plate. When the bacterial colonies with PHA granules were stained with nile blue,
the stained colonies fluoresced bright orange on the irradiation of UV light. In the fluoresce emission spectra, fluorescence
intensity increased with an increase in the PHA content of bacterial cells.Alcaligenes eutrophus andA.latus colonies with poly(3-hydroxybutyric acid) (PHB) homopolymer exhibited an emission maximum at 580nm on the excitation at 490nm.
On the other hand,Pseudomonas oleovorans andP.putida with medium-chain-length (mcl-) PHA copolymers of C6, C8 and C10 units exhibited an emission maximum at 570nm. 相似文献
70.
Hiroshi Takaku Tsunehiko Ito Shiro Yoshida Tomoni Aoki Erik De Clercq 《Nucleosides, nucleotides & nucleic acids》2013,32(4):793-802
Abstract The acyclic nucleosides 2 were treated with 2-chloro-3-methyl-1-oxa-3-aza-2-phosphacyclopentane (3) in the presence of diisopropylethylamine to give the corresponding phosphoramidite derivatives (4). The phosphoramidite intermediates (4) were oxidized with m-chloroperbenzoic acid to the phosphoramidate derivatives (5). Treatment of 5a,b with ZnBr2 in CH3NO2 gave the corresponding acyclic nucleoside cyclic phosphoramidates (6a,b). Attempts to desilylation of 5c by tetrabutylammonium fluoride (TBAF) resulted in opening of the phosphoramidate ring. The newly synthesized compounds were evaluated for antiviral and antitumor cell activity. 相似文献