Cloning and characterization of a rat-specific repetitive DNA sequence

A 2.1-kb EcoRI fragment of rat DNA has been cloned and sequenced. This fragment contained a repetitive element which was highly specific for rat DNA and widely dispersed throughout the rat genome. The repetitive element is homologous to a sequence found in the 3' end of the rat LINE family. Because of its high degree of species specificity and its heterodisperse distribution, this sequence provided a useful marker for rat DNA in DNA transfection experiments into mouse host cells.     

Apparatus for the automated measurement of the responses of humans to odorants

An automated system for the measurement of the psychophysicaland physiological responses of humans to odorant stimulationof the nose and eyes is described. All aspects of the generationand production of odor stimuli, the recording of physiologicaland psychophysical responses of the subjects and the storageof data are managed by an Apple He computer. Both the nasaland ocular olfactometers are based on electronic mass flow controllerswhich are used to control the ratios of volume flow rates ofclean and odorant-saturated air. The output of each olfactometeris measured by a photo-ionization detector. Odor stimuli aredelivered to custom-fitted face masks, that allow separate stimulationof the nose and eyes, through Teflon (rtm) flow valves. A videocamera records the responses of the eyes and a pneumotachograph,in combination with a pressure transducer, records changes inrespiratory behavior. An electronic mouse is used to enter thesubject's psychophysical responses directly into the computer.The advantages of this methodology and its current and potentialapplications are discussed.