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91.
H?Bukulmez AL?Matthews CM?Sullivan C?Chen MJ?Kraay RC?Elston RW?Moskowitz VM?Goldberg ML?WarmanEmail author 《Arthritis research & therapy》2005,8(1):R25
In order to determine whether there is a genetic component to hip or knee joint failure due to idiopathic osteoarthritis (OA),
we invited patients (probands) undergoing hip or knee arthroplasty for management of idiopathic OA to provide detailed family
histories regarding the prevalence of idiopathic OA requiring joint replacement in their siblings. We also invited their spouses
to provide detailed family histories about their siblings to serve as a control group. In the probands, we confirmed the diagnosis
of idiopathic OA using American College of Rheumatology criteria. The cohorts included the siblings of 635 probands undergoing
total hip replacement, the siblings of 486 probands undergoing total knee replacement, and the siblings of 787 spouses. We
compared the prevalence of arthroplasty for idiopathic OA among the siblings of the probands with that among the siblings
of the spouses, and we used logistic regression to identify independent risk factors for hip and knee arthroplasty in the
siblings. Familial aggregation for hip arthroplasty, but not for knee arthroplasty, was observed after controlling for age
and sex, suggesting a genetic contribution to end-stage hip OA but not to end-stage knee OA. We conclude that attempts to
identify genes that predispose to idiopathic OA resulting in joint failure are more likely to be successful in patients with
hip OA than in those with knee OA. 相似文献
92.
应用免疫组织化学SP法对4例饲喂黄曲霉毒素B1的Wistar大鼠肝组织中AFB1-DNA加合物的染色及常规HE染色发现,4例肝细胞核均出现异型性,但未见肝细胞坏死、增生灶及肝细胞癌;免疫组化揭示部分肝细胞核出现棕黄色、不均质状的AFB1-DNA加合物,阳性细胞数占10~85%。结果提示免疫组织化学方法可作为一种AFB1的定位方法,AFB1在动物致癌过程中AFB1-DNA加合物可能具有启动作用 相似文献
93.
94.
Endometrial suppressor cells in beef cattle 总被引:3,自引:0,他引:3
Endometrial cells were recovered post mortem from cyclic and pregnant crossbred beef cattle (n = 5 each) on Days 16 to 18 after estrus, and were evaluated for their ability to suppress lymphocyte responses and release suppressor factor into the culture medium. The suppressor factor was assessed for transforming growth factor-beta (TGF-beta) activity. In addition, Percoll was used to fractionate endometrial cells from Angus cows (n = 4) on Days 16 to 18 of pregnancy to determine the density of the suppressor cells. Endometrial cells from cyclic and pregnant cows suppressed lymphocyte proliferative responses and released suppressor factor into the culture medium. The suppressor factor exhibited TGF-beta activity. Suppressor activities tended to be greatest for fractionated cells with densities of 1.01 and 1.095 g/mL. In conclusion, the bovine endometrium contains low- and high-density suppressor cells capable of releasing suppressor factor. The factor seems to be associated with TGF-beta. 相似文献
95.
Abstract The development and physiology of cord-forming saprotrophic basidiomycetes, which form extensive and persistent mycelial networks in woodland ecosystems, can be conveniently studied on non-sterile soil in laboratory microcosms mimicking field conditions. Morphological responses of Phanerochaete velutina mycelial systems to resource encounters, and decay partitioning following encounters, varied according to whether simulated woody litter was unsterile or autoclaved and on whether encounter took place at the mycelial foraging front or behind the margin (simulating litter fall onto established systems in the field). Results show that encounter of discrete resources by P. velutina is rapidly communicated to the entire mycelial system; that resource capture takes high priority at the expense of continued system extension and decay-derived carbon reallocation; and that polarized growth toward newly encountered resources, previously considered to occur infrequently with this species, may be readily detected using image analysis techniques. Potential advantages of polarized development of P. velutina are discussed. 相似文献
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98.
Rosemarije Buitenhuis Les Shipp Cynthia Scott-Dupree Angela Brommit Wonhyo Lee 《Experimental & applied acarology》2014,62(2):171-180
Biological control in ornamental crops is challenging due to the wide diversity of crops and cultivars. In this study, we tested the hypothesis that trichome density on different host plants influences the behavior and performance of the predatory mite Amblyseius swirskii Athias-Henriot (Acari: Phytoseiidae). Behavioural observations of this predator in the presence or absence of prey (western flower thrips, Frankliniella occidentalis Pergande) (Thysanoptera: Thripidae) were done on leaf squares of ornamental plant species differing in trichome density (rose, chrysanthemum and gerbera) and compared to a smooth surface (plastic). Tomato leaves were used to observe the influence of glandular trichomes. The performance of A. swirskii was assessed by measuring predation and oviposition rate. Behaviour of A. swirskii was influenced by plant species. Up to a certain density of trichomes, trichome number had a negative effect on walking speed. It was highest on plastic, followed by rose. No differences were found among chrysanthemum, gerbera and tomato. Walking speed was slightly higher on disks without prey. Proportion of time spent walking was the same on leaf disks of all plant species, with and without prey. No effect of glandular trichomes on tomato leaves was seen. Most thrips were killed and consumed on gerbera, and least on rose. Predation rates on chrysanthemum and plastic were intermediate. In contrast, no differences in oviposition rate were found among plant species. The results of this study indicate that trichome density can explain some of the variability in efficacy of A. swirskii on different crops. Release rates of A. swirskii may need to be adjusted depending on the crop in which it is used. 相似文献
99.
Marleen TJ van Ampting Arjan J Schonewille Carolien Vink Robert Jan M Brummer van der Roelof Meer Ingeborg MJ Bovee-Oudenhoven 《BMC physiology》2009,9(1):6-9
Background
Glutathione, the main antioxidant of intestinal epithelial cells, is suggested to play an important role in gut barrier function and prevention of inflammation-related oxidative damage as induced by acute bacterial infection. Most studies on intestinal glutathione focus on oxidative stress reduction without considering functional disease outcome. Our aim was to determine whether depletion or maintenance of intestinal glutathione changes susceptibility of rats to Salmonella infection and associated inflammation. 相似文献100.
Michael Honer Thomas Ebenhan Peter R Allegrini Simon M Ametamey Mike Becquet Catherine Cannet Heidi A Lane Terence M O'Reilly Pius A Schubiger Melanie Sticker-Jantscheff Michael Stumm Paul MJ McSheehy 《Translational oncology》2010,3(4):264-275
Noninvasive functional imaging of tumors can provide valuable early-response biomarkers, in particular, for targeted chemotherapy. Using various experimental tumor models, we have investigated the ability of positron emission tomography (PET) measurements of 2-deoxy-2-[18F]fluoro-glucose (FDG) and 3′-deoxy-3′-[18F]fluorothymidine (FLT) to detect response to the allosteric mammalian target of rapamycin (mTOR) inhibitor everolimus. Tumor models were declared sensitive (murine melanoma B16/BL6 and human lung H596) or relatively insensitive (human colon HCT116 and cervical KB31), according to the IC50 values (concentration inhibiting cell growth by 50%) for inhibition of proliferation in vitro (<10 nM and >1 µM, respectively). Everolimus strongly inhibited growth of the sensitive models in vivo but also significantly inhibited growth of the insensitive models, an effect attributable to its known anti-angiogenic/vascular properties. However, although tumor FDG and FLT uptake was significantly reduced in the sensitive models, it was not affected in the insensitive models, suggesting that endothelial-directed effects could not be detected by these PET tracers. Consistent with this hypothesis, in a well-vascularized orthotopic rat mammary tumor model, other antiangiogenic agents also failed to affect FDG uptake, despite inhibiting tumor growth. In contrast, the cytotoxic patupilone, a microtubule stabilizer, blocked tumor growth, and markedly reduced FDG uptake. These results suggest that FDG/FLT-PET may not be a suitable method for early markers of response to antiangiogenic agents and mTOR inhibitors in which anti-angiogenic/vascular effects predominate because the method could provide false-negative responses. These conclusions warrant clinical testing. 相似文献