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We used the repetitive character of transposable elements to isolate a
non-LTR retrotransposon in Drosophila subobscura. bilbo, as we have called
it, has homology to TRIM and LOA elements. Sequence analysis showed a 5'
untranslated region (UTR), an open reading frame (ORF) with no RNA-binding
domains, a downstream ORF that had structural homology to that of the I
factor, and, finally, a 3' UTR which ended in several 5-nt repeats. The
results of our phylogenetic and structural analyses shed light on the
evolution of Drosophila non-LTR retrotransposons and support the hypothesis
that an ancestor of these elements was structurally complex.
相似文献
54.
Tufan G?kirmak Joseph P. Campanale Lauren E. Shipp Gary W. Moy Houchao Tao Amro Hamdoun 《The Journal of biological chemistry》2012,287(52):43876-43883
In this study, we cloned, expressed and functionally characterized Stronglycentrotus purpuratus (Sp) ATP-binding cassette (ABC) transporters. This screen identified three multidrug resistance (MDR) transporters with functional homology to the major types of MDR transporters found in humans. When overexpressed in embryos, the apical transporters Sp-ABCB1a, ABCB4a, and ABCG2a can account for as much as 87% of the observed efflux activity, providing a robust assay for their substrate selectivity. Using this assay, we found that sea urchin MDR transporters export canonical MDR susbtrates such as calcein-AM, bodipy-verapamil, bodipy-vinblastine, and mitoxantrone. In addition, we characterized the impact of nonconservative substitutions in the primary sequences of drug binding domains of sea urchin versus murine ABCB1 by mutation of Sp-ABCB1a and treatment of embryos with stereoisomeric cyclic peptide inhibitors (QZ59 compounds). The results indicated that two substitutions in transmembrane helix 6 reverse stereoselectivity of Sp-ABCB1a for QZ59 enantiomers compared with mouse ABCB1a. This suggests that subtle changes in the primary sequence of transporter drug binding domains could fine-tune substrate specificity through evolution. 相似文献
55.
I. Vänninen D.M. Pinto A.I. Nissinen N.S. Johansen L. Shipp 《The Annals of applied biology》2010,157(3):393-414
This review describes the effects of the current and emerging lighting technologies on plants, and the plant-mediated effects on herbivorous and beneficial arthropods in high-technology year-round greenhouse production, where light quality, quantity and photoperiod differ from the natural environment. The spectrum provided by the current lighting technology, high-pressure sodium lamp (HPSL), differs considerably from that of solar radiation. The major plant-mediated effects on arthropods were predicted to result from (a) extended photoperiods and lower light integrals, (b) the attenuation of ultraviolet (UV) wavelengths, particularly UV-B, (c) the high red: far-red (R : FR) ratio and lower blue : red (B : R) in comparison with solar radiation and (d) the high proportion of yellow wavelengths during winter months. Of these light factors (a–d) (ceteris paribus), (a) and (b) were hypothesised to result in increased performance of herbivores in winter months, whereas the high R : FR ratio decreased herbivore performance or not affected it, at least when interlights are used. The predictions obtained on the basis of this review are also discussed in relation to the modifying factors prevailing in these production environments: enriched CO2 levels, high nutrient amounts, optimised irrigation and temperatures optimal for plants' needs. Based on the carbon/nitrogen and growth/differentiation balance theories, these modifying factors tend to produce plants that allocate most resources to growth at the expense of defensive secondary metabolism and physicochemical defensive structures. At the end, this review discusses knowledge gaps and future research prospects, in which light-emitting diodes, the emerging lighting technology, play an important role by enabling the targeted manipulation of plant responses to different wavelengths. 相似文献
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A Luquita L Urli MJ Svetaz AM Gennaro ME Giorgetti G Pistone R Volpintesta S Palatnik M Rasia 《Journal of biomedical science》2010,17(1):8
Background
Hyaluronic acid (HA) is present in many tissues; its presence in serum may be related to certain inflammatory conditions, tissue damage, sepsis, liver malfunction and some malignancies. In the present work, our goal was to investigate the significance of hyaluronic acid effect on erythrocyte flow properties. Therefore we performed in vitro experiments incubating red blood cells (RBCs) with several HA concentrations. Afterwards, in order to corroborate the pathophysiological significance of the results obtained, we replicated the in vitro experiment with ex vivo RBCs from diagnosed rheumatoid arthritis (RA) patients, a serum HA-increasing pathology. 相似文献57.
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Shipp S 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2005,360(1456):797-814
The agranular cortex is an important landmark-anatomically, as the architectural flag of mammalian motor cortex, and historically, as a spur to the development of theories of localization of function. But why, exactly, do agranularity and motor function go together? To address this question, it should be noted that not only does motor cortex lack granular layer four, it also has a relatively thinner layer three. Therefore, it is the two layers which principally constitute the ascending pathways through the sensory (granular) cortex that have regressed in motor cortex: simply stated, motor cortex does not engage in serial reprocessing of incoming sensory data. But why should a granular architecture not be demanded by the downstream relay of motor instructions through the motor cortex? The scant anatomical evidence available regarding laminar patterns suggests that the pathways from frontal and premotor areas to the primary motor cortex actually bear a greater resemblance to the descending, or feedback connections of sensory cortex that avoid the granular layer. The action of feedback connections is generally described as "modulatory" at a cellular level, or "selective" in terms of systems analysis. By contrast, ascending connections may be labelled "driving" or "instructive". Where the motor cortex uses driving inputs, they are most readily identified as sensory signals instructing the visual location of targets and the kinaesthetic state of the body. Visual signals may activate motor concepts, e.g. "mirror neurons", and the motor plan must select the appropriate muscles and forces to put the plan into action, if the decision to move is taken. This, perhaps, is why "driving" motor signals might be inappropriate-the optimal selection and its execution are conditional upon both kinaesthetic and motivational factors. The argument, summarized above, is constructed in honour of Korbinian Brodmann's centenary, and follows two of the fundamental principles of his school of thought: that uniformities in cortical structure, and development imply global conservation of some aspects of function, whereas regional variations in architecture can be used to chart the "organs" of the cortex, and perhaps to understand their functional differences. 相似文献