全文获取类型
收费全文 | 1015篇 |
免费 | 47篇 |
专业分类
1062篇 |
出版年
2024年 | 2篇 |
2023年 | 3篇 |
2022年 | 7篇 |
2021年 | 12篇 |
2020年 | 7篇 |
2019年 | 13篇 |
2018年 | 15篇 |
2017年 | 12篇 |
2016年 | 18篇 |
2015年 | 39篇 |
2014年 | 30篇 |
2013年 | 74篇 |
2012年 | 74篇 |
2011年 | 54篇 |
2010年 | 51篇 |
2009年 | 38篇 |
2008年 | 77篇 |
2007年 | 80篇 |
2006年 | 57篇 |
2005年 | 65篇 |
2004年 | 46篇 |
2003年 | 41篇 |
2002年 | 36篇 |
2001年 | 13篇 |
2000年 | 10篇 |
1999年 | 13篇 |
1998年 | 6篇 |
1997年 | 14篇 |
1996年 | 12篇 |
1995年 | 3篇 |
1994年 | 2篇 |
1993年 | 4篇 |
1992年 | 10篇 |
1991年 | 10篇 |
1990年 | 11篇 |
1989年 | 15篇 |
1988年 | 11篇 |
1987年 | 7篇 |
1986年 | 12篇 |
1985年 | 8篇 |
1984年 | 5篇 |
1983年 | 9篇 |
1982年 | 8篇 |
1981年 | 3篇 |
1979年 | 6篇 |
1978年 | 3篇 |
1976年 | 3篇 |
1975年 | 2篇 |
1968年 | 2篇 |
1965年 | 1篇 |
排序方式: 共有1062条查询结果,搜索用时 0 毫秒
111.
Hagai Levine Hideo Mohri Anders Ekbom Liliana Ramos Geoff Parker Eduardo Roldan Luca Jovine Sabine Koelle Anna Lindstrand Simone Immler Sharon Mortimer David Mortimer Gerhard van der Horst Sumio Ishijima Natalie Aneck-Hahn Elisabetta Baldi Roelof Menkveld Susan A Rothmann Aleksander Giwercman Yvonne Giwercman Mats Holmberg Ulrik Kvist Lars Björndahl Rebecka Holmberg Stefan Arver John Flanagan Joël R Drevet 《Andrologie》2018,28(1):13
On the occasion of the XIIIth International Symposium on Spermatology held from 9 to 13 May 2018 in Stockholm (Sweden), participants (guest speakers and audience) collectively felt the need to make a public statement on the general issue of male reproductive health. Our intention is to raise awareness of what we believe is a neglected area of research despite alarming situations around the world. The disclosure strategy desired by the co-authors is to bring it to the attention of the greatest number partly by considering co-publication in the various periodicals dealing with Reproductive Biology and Andrology. BaCA’s editorial office accepted this mission and found it natural that our periodical, the official journal of the French Andrology Society (SALF), should carry this message. 相似文献
112.
Masaya Nakamura Jun Watanabe Ryokei Ogawa Shinsuke Kanamura 《Journal of molecular histology》1997,29(9):645-654
The immunohistochemical localization of type II and type I collagens was examined in the articular cartilage of the femoral
head of growing rats injected systemically with 5 mg kg−1 dexamethasone for 2 weeks every other day. The intensities of immunostaining
for type II collagen, measured by microphotometry, was highest in the flattened cell layer and high in the hypertrophic cell
layer, moderate in the proliferative cell and transitional cell layers and low in the superficial layer. After dexamethasone
administration, the intensities decreased markedly in the flattened cell layer and slightly in the hypertrophic cell layer,
although the decreases in other layers were negligible. The staining intensities for type I collagen were highest in the flattened
cell layer, low in the superficial and transitional cell layers and very low in the proliferative and hypertrophic cell layers.
After dexamethasone administration, the intensities increased markedly in the flattened cell layer and slightly in the superficial
and proliferative cell layers, but did not change in the transitional and hypertrophic cell layers. Thus, dexamethasone administration
caused a decrease in type II collagen and an increase in type I collagen in the matrix of the surface portion of articular
cartilage. The composition of isoforms of collagen in the matrix changed after the steroid administration. The results strongly
suggest that the shift in collagen composition from type II to type I predominance is a cause of the degeneration of the articular
cartilage after glucocorticoid administration.
This revised version was published online in November 2006 with corrections to the Cover Date. 相似文献
113.
Takashi Uesugi Kazumi Sano Yoshihiro Uesawa Yoji Ikegami Kiminori Mohri 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1997,703(1-2)
An isocratic HPLC method for the simple and selective determination of adenine nucleoside and nucleotides has been developed. The separation is achieved at room temperature by reversed-phase chromatography (Shiseido, Capcell Pak C18). A mixture of 0.1 M triethylamine (TEA) phosphate buffer and methanol (95:5, v/v) is used as a standard eluent. Influence of pH and concentrations of organic modifiers and TEA ion on capacity factors of adenine compounds has been investigated. It has been also found that the TEA ion in the eluent is adsorbed onto the reversed-phase surface. The results clearly demonstrate that ion-pair formation with TEA ion occurs probably both in the mobile phase and on the stationary phase and governs the retention of adenine and nucleotides in the present system. The HPLC system is applied to the analysis of adenine nucleotides formed as intermediates in the synthesis of 3′-phosphoadenosine 5′-phosphosulphate (PAPS) and to the assays of ATPases and 5′-nucleotidase activities in rat liver plasma membrane. This method is a new type of ion-pair reversed-phase HPLC system and is suitable for the separation of highly polar organic anions, especially for adenine nucleotides. 相似文献
114.
115.
Matsuzaki S Yasuda Y Kobayashi S Koyama Y Kawamoto K Katayama T Tohyama M 《Biochemical and biophysical research communications》2007,358(2):540-544
The present study has examined the effect of metals, such as iron and copper on the cytotoxicity of amyloid beta protein 1-40 (Abeta40). First, we showed that monomeric Abeta40 has stronger cytotoxicity than various type of aggregated Abeta40. Next we showed the addition of metals into the monomeric Abeta40 reduced the cytotoxicity of either monomeric Abeta40 or metals (iron and copper) although the addition of metals into monomeric Abeta40 resulted in a marked increase of aggregated form of Abeta40, which composed of beta-sheeted Abeta40 and Abeta40 aggregation not characterized by beta-sheet fibrils (coagrated Abeta40). Taken together, the metals and monomeric Abeta40 affect on each other and cause the reduction of their cell toxicity. 相似文献
116.
Suyama K Yoshioka M Akagawa M Murayama Y Horii H Takata M Yokoyama T Mohri S 《Biochemical and biophysical research communications》2007,356(1):245-248
Since variant Creutzfeldt-Jakob disease (vCJD) has been suspected to be attributable to the infectious agents associated with bovine spongiform encephalopathy (BSE), it is important to prevent the transmission of pathogenic forms of prion protein (PrP(Sc)) through contaminated feeding materials such as meat and bone meal (MBM). Here, we demonstrate that the Maillard reaction employing a formulation of glucose in combination with sodium hydrogen carbonates effectively reduced the infectivity (approximately 5.9-log reduction) of a scrapie-infected hamster brain homogenate. In addition to a bioassay, a protein misfolding cyclic amplification (PMCA) technique, in which PrP(Sc) can be amplified in vitro, was used as a rapid test for assessing PrP(Sc) inactivation. The PMCA analysis also indicated that the PrP(Sc) level in the infected material significantly decreased following the Maillard reaction. Therefore, the Maillard reaction can be employed for the decontamination of large amounts of byproducts such as MBM. 相似文献
117.
Toshihisa Kojima Ken-ichi Kozaki Shinsuke Saga Yoshio Hashizume Naoki Ishiguro Hisashi Iwata Osamu Miyaishi 《Journal of cellular biochemistry》1997,65(4):542-549
The kinetics of type I procollagen synthesis in a human osteosarcoma cell line, MG 63, were investigated after treatment with 1,25-dihydroxyvitamin D3 (1,25-(OH)2 D3), a hormonal inducer of phenotypic differentiation. Pulse label and chase experiments demonstrated greatly enhanced production and more rapid reduction of intracellular procollagen molecules in the 1,25-(OH)2 D3–treated cells as compared to the nontreated case. After a chase for 1 h, labeled procollagen was reduced by nine-tenths in 1,25-(OH)2 D3–treated cells, while half of the radioactivity still remained in nontreated cells. The expression rate of type I collagen, which was examined by pulse label experiment, was elevated in association with an increase in the mRNA coding for the type I collagen α1 chain by 1,25-(OH)2 D3 treatment. However, the amount of intracellular procollagen present after 4 h continuous labeling was almost the same, independent of the 1,25-(OH)2 D3 treatment. Thus, we conclude that strage of the molecule was not affected. The results therefore suggest an increase in both the synthesis and secretion of type I collagen. The 1,25-(OH)2 D3 treatment was also found to induce the α subunit of prolyl 4-hydroxylase and to be associated with an elevated level of hydroxyproline in the procollagen. Moreover, gelatinase B–resistant procollagen molecules, indicative of intracellular procollagen molecules in the stable triple helical form, were detected only in the 1,25-(OH)2 D3–treated cells. These data suggest more efficient proline hydroxylation is involved in rapid secretion of procollagen after hormone administration. The present evidence points to posttranslational control of procollagen synthesis. J. Cell. Biochem. 65:542–549. © 1997 Wiley-Liss Inc. 相似文献
118.
119.
Inoue Y Yasutake N Oshima Y Yamamoto Y Tomita T Miyoshi S Yatake T 《Bioscience, biotechnology, and biochemistry》2002,66(12):2594-2599
The maltose phosphorylase (MPase) gene of Bacillus sp. strain RK-1 was cloned by PCR with oligonucleotide primers designed on the basis of a partial N-terminal amino acid sequence of the purified enzyme. The MPase gene consisted of 2,655 bp encoding a theoretical protein with a Mr of 88,460, and had no secretion signal sequence, although most of the MPase activity was detected in the culture supernatant of RK-1. This cloned MPase gene and the trehalose phosphorylase (TPase) gene from Bacillus stearothermophilus SK-1 were efficiently expressed intracellularly under the control of the Bacillus amyloliquefaciens alpha-amylase promoter in Bacillus subtilis. The production yields were estimated to be more than 2 g of enzyme per liter of medium, about 250 times the production of the original strains, in a simple shake flask. About 60% of maltose was converted into trehalose by the simultaneous action of both enzymes produced in B. subtilis. 相似文献
120.
Shinsuke Fujihara Masuro Yamaguchi 《Bioscience, biotechnology, and biochemistry》2013,77(11):2569-2573
The activity of nitrogenase and the concentration of ammonia and allantoin (+ allantoic acid) in root nodules were measured throughout the growth period of soybean plants. Nitrogenase activity measured by acetylene reduction increased with plant growth and reached a maximum level at the flowering period. The level of ammonia and allantoin concentration in nodules was parallel with increased nitrogenase activity. At the late reproductive stage (pod-forming period), nitrogenase activity showed a marked decrease, but the ammonia and allantoin in the nodules remained at a constant level. Detached nodules from 56 day-old soybean plants were exposed to 15N2 gas, and the distribution of 15N among nitrogen compounds was investigated. Enrichment of 15N in allantoin and allantoic acid reached a fairly high level after 90 min of nitrogen fixation; ca. 22% of 15N in acid-soluble nitrogen compounds was incorporated into allantoin + allantoic acid. In contrast, enrichment of 15N in amide nitrogen was relatively low. No significant 15N was detected in the RNA fraction. The data suggested that ureide formation in nitrogen-fixing root nodules did not take place through the breakdown of nucleic acids, but directly associated with the assimilating system of biologically fixed nitrogen. 相似文献