全文获取类型
收费全文 | 1212篇 |
免费 | 53篇 |
出版年
2023年 | 5篇 |
2022年 | 10篇 |
2021年 | 23篇 |
2020年 | 15篇 |
2019年 | 11篇 |
2018年 | 35篇 |
2017年 | 21篇 |
2016年 | 34篇 |
2015年 | 55篇 |
2014年 | 68篇 |
2013年 | 106篇 |
2012年 | 93篇 |
2011年 | 73篇 |
2010年 | 51篇 |
2009年 | 48篇 |
2008年 | 58篇 |
2007年 | 97篇 |
2006年 | 63篇 |
2005年 | 78篇 |
2004年 | 77篇 |
2003年 | 64篇 |
2002年 | 50篇 |
2001年 | 8篇 |
2000年 | 5篇 |
1999年 | 10篇 |
1998年 | 11篇 |
1997年 | 18篇 |
1996年 | 5篇 |
1995年 | 10篇 |
1994年 | 7篇 |
1993年 | 10篇 |
1992年 | 7篇 |
1991年 | 3篇 |
1990年 | 3篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1987年 | 5篇 |
1986年 | 3篇 |
1985年 | 5篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 6篇 |
1981年 | 1篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1973年 | 2篇 |
1972年 | 1篇 |
1971年 | 1篇 |
1970年 | 1篇 |
排序方式: 共有1265条查询结果,搜索用时 31 毫秒
961.
962.
Tatsunori Masatani Ragab M. Fereig Konosuke Otomaru Shingo Ishikawa Isshu Kojima Seiji Hobo Yoshifumi Nishikawa 《Parasitology international》2018,67(6):763-767
Cryptosporidium parvum and Neospora caninum are common parasites in domesticated cattle worldwide, including in Japan. We carried out a serological survey to detect C. parvum and N. caninum infection among cattle in the southern Kyushu region of Japan—including the small islands—by indirect enzyme-linked immunosorbent assay based on recombinant antigens. We found that total seropositivity in 570 Japanese black cattle was 96.3% for C. parvum and 18.4% for N. caninum. Although seroprevalence was correlated with cattle age, differences in the seroprevalence of C. parvum among age groups were not statistically significant. On the other hand, N. caninum seroprevalence increased with age, suggesting horizontal transmission through ingestion of food or water contaminated with oocysts. These findings underscore the importance of monitoring C. parvum and N. caninum in cattle and implementing measures to prevent the spread of infection to other livestock and to humans. 相似文献
963.
Sastia Prama Putri Yasumune Nakayama Claire Shen Shingo Noguchi Katsuaki Nitta Takeshi Bamba Sammy Pontrelli James Liao Eiichiro Fukusaki 《Metabolomics : Official journal of the Metabolomic Society》2018,14(7):96
Introduction
Previously constructed Escherichia coli strains that produce 1-propanol use the native threonine pathway, or a heterologous citramalate pathway. However, based on the energy and cofactor requirements of each pathway, a combination of the two pathways produces synergistic effects that increase the theoretical maximum yield with a simultaneous unexplained increase in productivity.Objective
Identification of key factors that contribute to synergistic effect leading to 1-propanol yield and productivity improvement in E. coli with native threonine pathway and heterologous citramalate pathway.Method
A combination of snapshot metabolomic profiling and dynamic metabolic turnover analysis were used to identify system-wide perturbations that contribute to the productivity improvement.Result and Conclusion
In the presence of both pathways, increased glucose consumption and elevated levels of glycolytic intermediates are attributed to an elevated phosphoenolpyruvate (PEP)/pyruvate ratio that is known to increase the function of the native phosphotransferase. Turnover analysis of nitrogen containing byproducts reveals that ammonia assimilation, required for the threonine pathway, is streamlined when provided with an NAD(P)H surplus in the presence of the citramalate pathway. Our study illustrates the application of metabolomics in identification of factors that alter cellular physiology for improvement of 1-propanol bioproduction.964.
Corticotropin‐releasing hormone‐binding protein is up‐regulated by brain‐derived neurotrophic factor and is secreted in an activity‐dependent manner in rat cerebral cortical neurons
下载免费PDF全文
![点击此处可从《Journal of neurochemistry》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Naoki Adachi Shingo Suzuki Hidetada Matsuoka Satoko Fushimi Junichiro Ono Ken‐ichi Ohta Yohei Hirai Takanori Miki Hisatsugu Koshimizu 《Journal of neurochemistry》2018,146(1):99-110
965.
Shingo Miura 《Journal of Ethology》1984,2(2):69-75
Social behaviour and space use patterns of 15 male muntjacs (Muntiacus reevesi) were studied in a large enclosure in 1979 and 1980. Males often interacted aggressively with each other showing distinct
aggressive behaviour patterns. A dominance hierarchy was established and 4 adult males emerged as co-dominant. Movements of
these dominant males were confined within small areas which did not overlap with each other and partitioned the enclosure.
They defended and maintained the areas by aggressive behaviour and scent marking. 相似文献
966.
Mouse Prickle1 and Prickle2 are expressed in postmitotic neurons and promote neurite outgrowth 总被引:1,自引:0,他引:1
The Drosophila planar cell polarity (PCP) gene prickle has been previously indicated as one of the regulators of gastrulation in the early embryonic stage. However, the functional role of prickle in the brain in particular is not known. We first indicated that mouse Prickle1 and Prickle2 are continually expressed in the brain throughout the embryonic stages and are observed to be specifically expressed in the postmitotic neurons. Furthermore, Prickle1 or Prickle2 depletion effectively decreases the neurite outgrowth levels of mouse neuroblastoma Neuro2a cells. These results indicate that mouse Prickle1 and Prickle2 possibly regulate positive neurite formation during brain development. 相似文献
967.
Mindorff EN O'Keefe DD Labbé A Yang JP Ou Y Yoshikawa S van Meyel DJ 《Genetics》2007,176(4):2247-2263
968.
Arakawa T Kawano Y Kataoka S Katayama Y Kamiya N Yohda M Odaka M 《Journal of molecular biology》2007,366(5):1497-1509
Thiocyanate hydrolase (SCNase) of Thiobacillus thioparus THI115 is a cobalt(III)-containing enzyme catalyzing the degradation of thiocyanate to carbonyl sulfide and ammonia. We determined the crystal structures of the apo- and native SCNases at a resolution of 2.0 A. SCNases in both forms had a conserved hetero-dodecameric structure, (alphabetagamma)(4). Four alphabetagamma hetero-trimers were structurally equivalent. One alphabetagamma hetero-trimer was composed of the core domain and the betaN domain, which was located at the center of the molecule and linked the hetero-trimers with novel quaternary interfaces. In both the apo- and native SCNases, the core domain was structurally conserved between those of iron and cobalt-types of nitrile hydratase (NHase). Native SCNase possessed the post-translationally modified cysteine ligands, gammaCys131-SO(2)H and gammaCys133-SOH like NHases. However, the low-spin cobalt(III) was found to be in the distorted square-pyramidal geometry, which had not been reported before in any protein. The size as well as the electrostatic properties of the substrate-binding pocket was totally different from NHases with respect to the charge distribution and the substrate accessibility, which rationally explains the differences in the substrate preference between SCNase and NHase. 相似文献
969.
The mechanisms of autoimmune disease have remained puzzling for a long time. Here we construct a simple mathematical model for autoimmune disease based on the personal immune response function and the target cell growth function. We show that these two functions are sufficient to capture the essence of autoimmune disease and can explain characteristic symptom phases such as tolerance, repeated flare-ups and dormancy. Our results strongly suggest that a more complete understanding of these two functions will underlie the development of an effective therapy for autoimmune disease. 相似文献
970.
A novel method to link a nascent protein (phenotype) to its mRNA (genotype) covalently through the N-terminus was developed. The mRNA harboring amber stop codon at just downstream of initiation site was hybridized with hydrazide-modified ssDNA at upstream of coding region and was ligated to the DNA. This construct was then modified with 4-acetyl-phenylalanyl amber suppressor tRNA. This modified construct was fused with the nascent protein via the phenylalanine derivative when the mRNA uses the amber suppressor tRNA to decode the amber stop codon. The obtained fusion molecule was used successfully in selective enrichment experiments. It will be applicable for high-through-put screening in evolutionary protein engineering. In contrast to fusion molecules generated by other methods in which the protein is linked to genotype molecule through the C- terminus, our fusion molecule will serve to select a protein for which the C-terminus is essential to be active. 相似文献