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排序方式: 共有5462条查询结果,搜索用时 15 毫秒
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Feiran Zhang Christy Hammack Sarah C. Ogden Yichen Cheng Emily M. Lee Zhexing Wen Xuyu Qian Ha Nam Nguyen Yujing Li Bing Yao Miao Xu Tianlei Xu Li Chen Zhiqin Wang Hao Feng Wei-Kai Huang Ki-jun Yoon Chao Shan Luoxiu Huang Zhaohui Qin Kimberly M. Christian Pei-Yong Shi Mingjiang Xu Menghang Xia Wei Zheng Hao Wu Hongjun Song Hengli Tang Guo-Li Ming Peng Jin 《Nucleic acids research》2016,44(18):8610-8620
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S Y Choi F Kwok J H Bahn S G Jeon Y K Ahn B H Yoon B R Lee K S Choi G Z Gao 《BioFactors (Oxford, England)》1999,10(1):35-42
Six monoclonal antibodies that recognize porcine brain pyridoxal kinase have been selected and designated as PK67, PK86, PK91, PK144, PK252 and PK275. A total of six monoclonal antibodies recognizing different epitopes of the enzyme were obtained, of which four inhibited the enzyme activity. When total proteins of porcine brain homogenate separated by SDS-PAGE were subjected to monoclonal antibodies, a single reactive protein band of molecular weight 39 kDa which comigrated with purified porcine pyridoxal kinase was detected. Using the anti-pyridoxal kinase antibodies as probes, the cross reactivities of brain pyridoxal kinase from human and other mammalian tissues and from avian sources were also investigated. Among human and all animal tissues tested, immunoreactive bands on Western blots appeared to have the same molecular mass of 39 kDa. These results indicate that mammalian brains contain only one major type of immunologically similar pyridoxal kinase, although some properties of the enzymes reported previously differed from one another. 相似文献
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Yong-Il Park Byung-Dae Yoon Yuan Chuan Lee 《Biotechnology and Bioprocess Engineering》1999,4(3):165-169
Baculovirus expression vector insect cell system (BEVIS) is useful for high level production of human therapeutic proteins.
However, it has been reported that recombinant glycoproteins produced from this system are, in many cases, biologically inactive
or less active than authentic counterparts, due to incomplete glycosylation potential of insect cells used so far, producing
recombinant proteins with only high-or paucimannosidic oligosaccharides without sialylation. The presence of sialic acids
in insects is still controversial. Egg proteins ofTrichoplusia ni andDanaus plexippus were isolated, and the presence of sialic acids was examined using reverse-phase fluorescent HPLC after derivatization of
samples with 1,2-diamino-4,5-methylenedioxybenzene (DMB). The proteins of both eggs were shown to contain 5-N-acetylneuraminic acid. The results suggest that both insects may be able to produce proteins with sialylated complex-type
oligosaccharide chains. 相似文献
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Cloning of the Human Monocarboxylate Transporter MCT3 Gene: Localization to Chromosome 22q12.3–q13.2
Lactate transport across cell membranes is mediated by a family of proton-coupled monocarboxylate transporters (MCTs). The retinal pigment epithelium (RPE) expresses a unique member of this family, MCT3. A portion of the human MCT3 gene was cloned by polymerase chain reaction using primers designed from rat RPE MCT3 cDNA sequence. The human genomic sequence was used to design primers to clone human MCT3 cDNA and to identify a bacterial artificial chromosome clone containing the human MCT3 gene. The human MCT3 cDNA contained a 1512-nucleotide open reading frame with a deduced amino sequence 85% identical to rat MCT3. Comparison of the cDNA and genomic sequences revealed that the MCT3 gene was composed of five exons distributed over 5 kb of DNA. The exon–intron borders were conserved between the human and the chicken MCT3 genes. Using radiation hybrid mapping, the MCT3 gene was mapped to chromosome 22 between markers WI11639 and SGC30687. A search of chromosome 22 in the Sanger Centre database confirmed the location of the human MCT3 gene at 22q12.3–q13.2. 相似文献