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681.
Plasma amino acid patterns in hepatocellular carcinoma 总被引:3,自引:0,他引:3
C Hirayama K Suyama Y Horie K Tanimoto S Kato 《Biochemical medicine and metabolic biology》1987,38(2):127-133
Plasma amino acid levels were determined in 23 patients in comparison with 16 normal subjects and 17 patients with liver cirrhosis. Patients with hepatocellular carcinoma had elevated levels of the aromatic amino acids and lowered levels of the branched-chain amino acids, as seen in liver cirrhosis; however, they had lowered levels of alanine and glutamine as compared with normal subjects and with liver cirrhosis patients. Following treatment with intraarterial chemotherapy and/or transcatheter arterial embolization, plasma levels of alanine and glutamine recovered. These results suggest that the consumption of alanine and glutamine increase in hepatocellular carcinoma. 相似文献
682.
Tanimoto Shizufumi; Satoh Shinobu; Fujii Tadashi; Harada Hiroshi 《Plant & cell physiology》1984,25(7):1161-1167
Application of di-isopropyl fluorophosphate (DFP), a highlysensitive inhibitor for serine enzymes, strongly inhibited cytokinin-inducedadventitious bud initiation in Torenia stem segments culturedin vitro. The inhibitory effect was not evident when DFP wasapplied after 3 days of culture. Amount of DFP-binding proteinsremarkably increased in superficial tissues of explants culturedfor 3 and 4 days on a medium containing benzyladenine. At least14 kinds of DFP-binding polypeptides were detected by SDS-polyacrylamidegel electrophoresis and fluorography. DFP-binding to some ofthese polypeptides was inhibited by a prior treatment with phenylmethylsulfonylfluoride and N-p-tosyl-L-lysine chloromethyl ketone. From theseresults, it was suggested that some serine proteases might berelated with biochemical events occurring during the initialstage of adventitious bud differentiation in Torenia stem segments. (Received May 8, 1984; Accepted July 5, 1984) 相似文献
683.
684.
685.
Inhibition by lactoferrin and kappa-casein glycomacropeptide of binding of Cholera toxin to its receptor. 总被引:1,自引:0,他引:1
Y Kawasaki H Isoda M Tanimoto S Dosako T Idota K Ahiko 《Bioscience, biotechnology, and biochemistry》1992,56(2):195-198
Inhibition from binding of Cholera toxin (CT) to Chinese hamster ovary (CHO)-K1 cells and ganglioside GM1 by lactoferrin (Lf) and kappa-casein glycomacropeptide (GMP) from cow's milk was examined. Both Lf and GMP effectively reduced the CT-derived morphological changes in CHO-K1 cells. The competitive binding assay demonstrated that both Lf and GMP inhibited the binding of CT to GM1, although their affinity for CT was lower than that of GM1. The inhibitory effect of Lf and GMP seemed to be attributed to their terminal sialic acid, although the sugar chain sequence only partially fitted to the CT-receptor. 相似文献
686.
Naoki Osumi Yoshihiro Kakehashi Shiho Matsumoto Kazunari Nagaoka Junichi Sakai Kiyotaka Miyashita Makoto Kimura Susumu Asakawa 《Archaea (Vancouver, B.C.)》2008,2(3):185-191
The gene sequences encoding disaggregatase (Dag), the enzyme
responsible for dispersion of cell aggregates of
Methanosarcina mazei to single cells, were determined
for three strains of M. mazei (S-6T, LYC
and TMA). The dag genes of the three strains were
3234 bp in length and had almost the same sequences with 97% amino
acid sequence identities. Dag was predicted to comprise 1077 amino
acid residues and to have a molecular mass of 120 kDa containing three
repeats of the DNRLRE domain in the C terminus, which is specific to
the genus Methanosarcina and may be responsible for
structural organization and cell wall function. Recombinant Dag was
overexpressed in Escherichia coli and preparations of
the expressed protein exhibited enzymatic activity. The RT-PCR
analysis showed that dag was transcribed to mRNA in
M. mazei LYC and indicated that the gene was
expressed in vivo. This is the first time the gene involved in the
morphological change of Methanosarcina spp. from
aggregate to single cells has been identified. 相似文献
687.
In order to facilitate the purification of 1,2-alpha-mannosidase from an enzyme product of Aspergillus oryzae, we have devised a rapid and simple procedure. A partially purified enzyme preparation obtained from the A. oryzae enzyme product, by means of ammonium sulfate fractionation followed by CM-Sephadex C-50 chromatography, was subjected to affinity chromatography with baker's yeast mannan gel as an adsorbent. 1,2-alpha-Mannosidase was retarded and well separated from the major protein peak on the affinity column. After a second affinity chromatography under the same conditions, 1,2-alpha-mannosidase was finally purified 7,500-fold with a 22.9% yield. The enzyme preparation thus obtained was quite suitable for the structural analysis of glycoconjugates. 相似文献
688.
689.
Active meristematic divisions in stem segments of Torenia culturedin vitro can be induced in the epidermis by application of cytokininor the calcium ionophore A23187
[GenBank]
, resulting in the differentiationof adventitious buds. Endogenous free glutamine accumulatedat a high concentration in the epidermal tissues during theearly stages of such cultures. The accumulation of glutaminewas caused by an increase in glutamine synthetase (GS) activity,and the increase of GS activity was suppressed by the applicationof some inhibitors of GS activity, mRNA synthesis, protein synthesis,or calmodulin. Incorporation of these inhibitors into the culturemedium also inhibited initiation of adventitious buds. The inhibitoryeffect of an inhibitor of GS, methionine sulfoximine (MSX),was apparent only at the very begining of the culture, and theeffect could be overcome by the simultaneous addition of glutamine.The inhibitory action of MSX on initiation of buds seemed tobe caused by an accumulation of ammonium ions. Reduction inlevels of NH4NO3 in or its elimination from the culture mediumstimulated the initiation of adventitious buds. Therefore, boththe accumulation of glutamine and the reduction in levels ofammonium ions seem to play a role in the initiation of adventitiousbuds in stem., segments of Torenia.
1Present address: Faculty of Agriculture, University of Saga,Honjo-cho, Saga, Saga, 840 Japan. (Received October 3, 1988; Accepted March 9, 1989) 相似文献
690.
Chihiro Motozono Mako Toyoda Jiri Zahradnik Akatsuki Saito Hesham Nasser Toong Seng Tan Isaac Ngare Izumi Kimura Keiya Uriu Yusuke Kosugi Yuan Yue Ryo Shimizu Jumpei Ito Shiho Torii Akiko Yonekawa Nobuyuki Shimono Yoji Nagasaki Rumi Minami Kei Sato 《Cell host & microbe》2021,29(7):1124-1136.e11
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