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171.
The Evolutionarily Significant Unit (ESU) was conceptualized in 1986 as a conservation unit below the species level, theoretically
applicable to a wide range of taxa. The concept has gained support, and various definitions or criteria, some of which are
inconsistent with each other, have since been proposed. Recent critiques of the ESU have pointed out the dominance of definitions
biased to the identification of long-term isolation or neutral genetic variation, which has largely ignored the adaptive components.
We present here the validity of such claims and show how the ESU definitions have actually been applied in research. We surveyed
scientific journals for original papers supporting ESU designations and determined who among the proponents of ESU definitions
have gained wider support. Our results indicate that indeed there are inconsistencies with the original concept and with the
existing definitions. Although the original concept recommended both ecological and genetic data as the basis for identification
of ESUs, which reflect true evolutionary variation, recent definitions have become biased to either neutral genetic variation
or adaptive variation. The definition which uses genetic data to assess neutral genetic variation (long-term isolation) has
gained major support, and therefore validates the earlier claims. To bridge the gap between the original concept and the practical
application, we propose the use of partial ESU and full ESU designations. The application of full ESU should be limited solely
to when both information about neutral genetic variation and adaptive variation are available. In other cases, in which only
a part of the variation is examined, we should use the term partial ESU (e.g., molecular-based ESU) and continue to investigate
focal populations from other aspects of variations to designate full ESU. 相似文献
172.
Immunohistochemical detection of hypoxia in mouse liver tissues treated with pimonidazole using “in vivo cryotechnique” 总被引:2,自引:2,他引:0
Wang J Olin M Rozell B Björkhem I Einarsson C Eggertsen G Gåfvels M 《Histochemistry and cell biology》2007,127(3):253-261
To evaluate hypoxic cells in live mouse liver tissues, immunohistochemistry for protein adducts of reductively activated pimonidazole
(PARaPi) was performed using the “in vivo cryotechnique (IVCT)” followed by freeze-substitution fixation. This method was
used because cryotechniques have some merits for examining biological events in living animal organs with improved time-resolution
compared to conventional perfusion and/or immersion chemical fixation. Pimonidazole was intraperitoneally injected into living
mice, and then after various times of hypoxia, their livers were quickly frozen by IVCT. The frozen liver tissues were freeze-substituted
in acetone containing 2% paraformaldehyde, and routinely embedded in paraffin wax. De-paraffinized sections were immunostained
for PARaPi. In liver tissues of mice without hypoxia, almost no immunostained cells were detected. However, in liver tissues
with 30 s of hypoxia, some hepatocytes in the pericentral zones were strongly immunostained. After 60 s of hypoxia, many hepatocytes
were immunostained with various degrees of staining intensity in all lobular zones, indicating different reactivities of pimonidazole
in the hepatocytes to hypoxia. At this time, the general immunoreactivity also appeared to be stronger around the central
veins than other portal areas. Although many hepatocytes were immunostained for PARaPi in the liver tissues with perfusion
fixation via heart, those with perfusion via portal vein were not immunostained. Thus, IVCT is useful to detect time-dependent
hypoxic states with pimonidazole treatment in living animal organs. 相似文献
173.
Takahito Mukai Atsushi Yamaguchi Kazumasa Ohtake Mihoko Takahashi Akiko Hayashi Fumie Iraha Satoshi Kira Tatsuo Yanagisawa Shigeyuki Yokoyama Hiroko Hoshi Takatsugu Kobayashi Kensaku Sakamoto 《Nucleic acids research》2015,43(16):8111-8122
The immutability of the genetic code has been challenged with the successful reassignment of the UAG stop codon to non-natural amino acids in Escherichia coli. In the present study, we demonstrated the in vivo reassignment of the AGG sense codon from arginine to l-homoarginine. As the first step, we engineered a novel variant of the archaeal pyrrolysyl-tRNA synthetase (PylRS) able to recognize l-homoarginine and l-N6-(1-iminoethyl)lysine (l-NIL). When this PylRS variant or HarRS was expressed in E. coli, together with the AGG-reading tRNAPylCCU molecule, these arginine analogs were efficiently incorporated into proteins in response to AGG. Next, some or all of the AGG codons in the essential genes were eliminated by their synonymous replacements with other arginine codons, whereas the majority of the AGG codons remained in the genome. The bacterial host''s ability to translate AGG into arginine was then restricted in a temperature-dependent manner. The temperature sensitivity caused by this restriction was rescued by the translation of AGG to l-homoarginine or l-NIL. The assignment of AGG to l-homoarginine in the cells was confirmed by mass spectrometric analyses. The results showed the feasibility of breaking the degeneracy of sense codons to enhance the amino-acid diversity in the genetic code. 相似文献
174.
Wakana Tanaka Yoshihiro Ohmori Tomokazu Ushijima Hiroaki Matsusaka Tomonao Matsushita Toshihiro Kumamaru Shigeyuki Kawano Hiro-Yuki Hirano 《The Plant cell》2015,27(4):1173-1184
Axillary shoot formation is a key determinant of plant architecture. Formation of the axillary shoot is regulated by initiation of the axillary meristem or outgrowth of the axillary bud. Here, we show that rice (Oryza sativa) TILLERS ABSENT1 (TAB1; also known as Os WUS), an ortholog of Arabidopsis thaliana WUS, is required to initiate axillary meristem development. We found that formation of the axillary meristem in rice proceeds via a transient state, which we term the premeristem, characterized by the expression of OSH1, a marker of indeterminate cells in the shoot apical meristem. In the tab1-1 (wus-1) mutant, however, formation of the axillary meristem is arrested at various stages of the premeristem zone, and OSH1 expression is highly reduced. TAB1/WUS is expressed in the premeristem zone, where it shows a partially overlapping pattern with OSH1. It is likely, therefore, that TAB1 plays an important role in maintaining the premeristem zone and in promoting the formation of the axillary meristem by promoting OSH1 expression. Temporal expression patterns of WUSCHEL-RELATED HOMEOBOX4 (WOX4) indicate that WOX4 is likely to regulate meristem maintenance instead of TAB1 after establishment of the axillary meristem. Lastly, we show that the prophyll, the first leaf in the secondary axis, is formed from the premeristem zone and not from the axillary meristem. 相似文献
175.
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178.
179.
Yamamoto H Ben S Saitoh S Kamata K Iguchi K Hoshino M 《Protein and peptide letters》2011,18(12):1204-1211
Galanin is a neuropeptide that is widely distributed in the central and peripheral nervous systems. In a previous study, we showed that a small cell lung carcinoma (SCLC) cell line, SBC-3A, released progalanin but not galanin, and that progalanin was then converted to galanin(1-20), the active form. Because the galanin(1-20) had undergone hydrolysis at Arg and Lys residues, the protease concerned was surmised to have a trypsin-like activity. The present study was performed to identify the trypsin-like protease which had previously been found to activate progalanin in this tumor tissue. The protease was isolated using chromatography and electrophoresis, and identified in tumor extracts from SBC-3A tumor-bearing mice; the major protease was found to be plasmin. We next confirmed that extracellular processing of progalanin occurs in SCLC tumor tissue (tumors produced by the implantation of SBC-3A cells into mice), and in two types of breast tumor tissue (obtained by implantation into mice of BT-549 and MDA-MB-436 cells). In cell culture, processed forms of progalanin were undetectable in SBC-3A, BT-549 or MDA-MB-436 cells. Conversely, gel filtration chromatography analysis of tumor extracts from SBC-3A, BT-549 and MDA-MB-436-bearing mice, revealed that galanin-like immunoreactivity (galanin-LI) in these tumor extracts was due to the presence of progalanin (14 kDa) and galanin(1-20) (2 kDa). Moreover, trypsin-like protease activity was elevated, and plasmin was expressed abundantly in SBC-3A, BT-549 and MDA-MB-436 tumors in mice. In addition, tranexamic acid, a plasmin inhibitor, inhibited progalanin conversion to galanin(1-20). The present study revealed that plasmin was present in tumor tissue, and that it was responsible for processing progalanin to galanin(1-20) in the extracellular environment. 相似文献
180.
Kuratani M Kasai T Akasaka R Higashijima K Terada T Kigawa T Shinkai A Bessho Y Yokoyama S 《Proteins》2011,79(7):2065-2075
The hypermodified nucleoside N6‐threonylcarbamoyladenosine resides at position 37 of tRNA molecules bearing U at position 36 and maintains translational fidelity in the three kingdoms of life. The N6‐threonylcarbamoyl moiety is composed of L ‐threonine and bicarbonate, and its synthesis was genetically shown to require YrdC/Sua5. YrdC/Sua5 binds to tRNA and ATP. In this study, we analyzed the L ‐threonine‐binding mode of Sua5 from the archaeon Sulfolobus tokodaii. Isothermal titration calorimetry measurements revealed that S. tokodaii Sua5 binds L ‐threonine more strongly than L ‐serine and glycine. The Kd values of Sua5 for L ‐threonine and L ‐serine are 9.3 μM and 2.6 mM, respectively. We determined the crystal structure of S. tokodaii Sua5, complexed with AMPPNP and L ‐threonine, at 1.8 Å resolution. The L ‐threonine is bound next to AMPPNP in the same pocket of the N‐terminal domain. Thr118 and two water molecules form hydrogen bonds with AMPPNP in a unique manner for adenine‐specific recognition. The carboxyl group and the side‐chain hydroxyl and methyl groups of L ‐threonine are buried deep in the pocket, whereas the amino group faces AMPPNP. The L ‐threonine is located in a suitable position to react together with ATP for the synthesis of N6‐threonylcarbamoyladenosine. Proteins 2011. © 2011 Wiley‐Liss, Inc. 相似文献