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121.
Yasukazu Nakamura Takakazu Kaneko Shusei Sato Mamoru Mimuro Hideaki Miyashita Tohru Tsuchiya Shigemi Sasamoto Akiko Watanabe Kumiko Kawashima Yoshie Kishida Chiaki Kiyokawa Mitsuyo Kohara Midori Matsumoto Ai Matsuno Naomi Nakazaki Sayaka Shimpo Chie Takeuchi Manabu Yamada Satoshi Tabata 《DNA research》2003,10(4):181-201
122.
123.
Hironobu Tsuchida Masahiko Kōmoto Hiromichi Kato Masao Fujimaki 《Bioscience, biotechnology, and biochemistry》2013,77(2):403-409
The hydrolyzate of the melanoidin prepared from glucose-ammonia system (kept in pH 5.3~6.0 during the reaction) was fractionated into the two fractions of non-adsorbate and adsorbate on Amberlite IR-120 (H+-form). In the present paper, the adsorbed fraction (Fraction B) was examined.Paper chromatographic examination of the Fraction B indicated the presence of at least eight compounds positive to diazotized sulphanilic acid reagent. The two compounds of them (indicated orange and orange-yellow color) were isolated and identified as 2-methyl-5-hydroxy-pyridine and 2-hydroxymethyl-5-hydroxy-pyridine, respectively.It is probable that these compounds would loosely be bound as a small moiety in the melanoidin molecule. 相似文献
124.
Fundamental studies on the cultural conditions for the amino acids production from pentoses and hexoses employing a strain of our new isolates named Brevibacterium pentoso-aminoacidicum nov. sp. were carried out.As a result of these experiments, it became possible to obtain about 30% of alanine and 10% of L-glutamic acid based on xylose, and alanine from glucose with a yield of about 40% in the proper conditions. 相似文献
125.
Teruaki Shiroza Naoyuki Ebisawa Atsuko Kojima Keiko Furihata Akira Shimazu Toyoshige Endō 《Bioscience, biotechnology, and biochemistry》2013,77(7):1885-1890
The new peptide antibiotics have been obtained from the culture filtrate of a streptomycete, strain 248-Sq2, isolated from a soil sample collected at Nagano Prefecture, Japan.On the basis of taxonomic studies, the producing organisms is designated as Streptomyces cirratus. Two active principles are named cirratiomycin A and B.These antibiotics exhibit a narrow range of activities against Lactobacillus casei and some strains of Streptococcus and Mycobacterium. 相似文献
126.
Kunio Katō 《Bioscience, biotechnology, and biochemistry》2013,77(6):657-663
The volatile compounds such as acetaldehyde, propionaldehyde, acrolein, acetone, diacetyl, furan, furfural and 5-methyl furfural from cellulose, cellobiose, glucose and levoglucosan pyrolysates at 250°C, 350°C and 500°G were studied by gas chromatography with a pyrolyzer without intermediate trapping. The composition of the volatiles was changed with the temperatures and the degradation stages of cellulose pyrolysis.Analytical data of the relative amounts of the volatiles show that pyrolysis of cellulose proceeds through two primary simultaneous reactions: a) the initial scission of glucosidic linkages, and b) chemical changes in anhydroglucose units of cellulose. 相似文献
127.
Chang Han Kim Toyoshige Endō Hiroshi Yonehara 《Bioscience, biotechnology, and biochemistry》2013,77(8):1673-1674
Myoglobin (Mb) purified from fast skeletal muscle of bluefin tuna Thunnus thynnus orientalis was subjected to thermal treatment, and the denaturation profiles were examined by thermodynamic analysis. Based on the ellipticity or helical content obtained by circular dichroism (CD) spectrometry, it was found that denaturation of tuna Mb consisted of three steps, and that slight structural changes of Mb started below 20 °C. However, major structural changes were observed at around 58 and 72 °C. Differential scanning calorimetry (DSC) analysis revealed a similar but somewhat different thermal denaturation profile of Mb. In comparison with the denaturing profiles of whale Mb under the same conditions, the thermal stability of tuna Mb was found to be much lower. In the modeled tertiary structures of these Mbs, they were roughly similar to each other, though minor conformational differences were recognized and the total energy was found to be lower for tuna Mb. 相似文献
128.
Bax-inhibiting peptide derived from mouse and rat Ku70 总被引:5,自引:0,他引:5
Yoshida T Tomioka I Nagahara T Holyst T Sawada M Hayes P Gama V Okuno M Chen Y Abe Y Kanouchi T Sasada H Wang D Yokota T Sato E Matsuyama S 《Biochemical and biophysical research communications》2004,321(4):961-966
Bax is a proapoptotic protein that plays a key role in the induction of apoptosis. Ku70 has activities to repair DNA damage in the nucleus and to suppress apoptosis by inhibiting Bax in the cytosol. We previously designed peptides based on the amino acid sequence of Bax-binding domain of human Ku70, and showed that these peptides bind Bax and inhibit cell death in human cell lines. In the present report, we examined the biological activities of other pentapeptides, VPTLK and VPALR, derived from mouse and rat Ku70. Cells in culture accumulated FITC-labeled VPTLK and VPALR, indicating that these peptides are cell permeable (human, mouse, rat, and porcine cells were examined). These peptides bound to Bax and suppressed cell death in various cell types including primary cultured cells. These data suggest that such Bax inhibiting peptides from three mammalian species may be used to protect healthy cells from apoptotic injury under pathological conditions. 相似文献
129.
Kunio Katō Fumiko Watanabe Shigeru Eda 《Bioscience, biotechnology, and biochemistry》2013,77(3):533-538
The structure of an arabinogalactan, separated from extracellular polysaccharides of cultured tobacco cells, has been investigated by methylation analysis of the original polysaccharide and of the products obtained after mild acid hydrolysis and after controlled Smith degradation.The arabinogalactan consists of l-arabinose, d-galactose and l-rhamnose in the molar ratio of 47: 45: 8. The arabinogalactan has a main chain of (1→3)-linked d-galactopyranosyl residues, half of which are substituted at the 6-position. Most of the side chains consist of three (1→6)-linked D-galactopyranosyl residues, to which l-arabinose residues are attached at C-3. The l-arabinofuranosyl and pyranosyl residues are present as end groups, and l-arabinopyranosyl residues are attached to C-5 of l-arabinofuranosyl residues. Non-reducing terminal l-rhamnopyranosyl residues are also present. 相似文献
130.