全文获取类型
收费全文 | 293篇 |
免费 | 11篇 |
出版年
2022年 | 3篇 |
2021年 | 2篇 |
2020年 | 2篇 |
2019年 | 4篇 |
2018年 | 4篇 |
2016年 | 5篇 |
2015年 | 6篇 |
2014年 | 10篇 |
2013年 | 40篇 |
2012年 | 11篇 |
2011年 | 22篇 |
2010年 | 11篇 |
2009年 | 11篇 |
2008年 | 17篇 |
2007年 | 15篇 |
2006年 | 13篇 |
2005年 | 24篇 |
2004年 | 14篇 |
2003年 | 12篇 |
2002年 | 8篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1999年 | 8篇 |
1998年 | 3篇 |
1997年 | 4篇 |
1996年 | 1篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1981年 | 3篇 |
1980年 | 2篇 |
1979年 | 6篇 |
1978年 | 3篇 |
1977年 | 5篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1974年 | 3篇 |
1972年 | 1篇 |
1966年 | 3篇 |
1965年 | 2篇 |
排序方式: 共有304条查询结果,搜索用时 31 毫秒
141.
Masakazu Kogawa Koji Hisatake Gerald J. Atkins David M. Findlay Yuichiro Enoki Tsuyoshi Sato Peter C. Gray Yukiko Kanesaki-Yatsuka Paul H. Anderson Seiki Wada Naoki Kato Aya Fukuda Shigehiro Katayama Masafumi Tsujimoto Tetsuya Yoda Tatsuo Suda Yasushi Okazaki Masahito Matsumoto 《The Journal of biological chemistry》2013,288(43):31299-31312
142.
Segmentation is a body-patterning strategy in which new segments are specified from a segment-addition zone containing uncommitted cells. However, the cell-recruitment process is poorly understood. Here we investigated in detail the segmentation in a polychaete annelid, Perinereis nuntia (Lophotrochozoa), in which new segments emerge at the boundary between the posterior end of the segmented region and the terminal pygidium. Cells at this border synchronously remodel their chromatin, enter the cell cycle, and undergo oriented cell division, before being added to new segments. wingless is expressed at the posterior edge of the pre-existing segment, abutted by hedgehog in the first row of the new segment. Overstimulation of Wingless signaling caused excess cells to enter the cell cycle, prolonging segmentation and widening the new segment. Thus, segment addition may occur by a homeogenetic mechanism, in which Wingless expressed in the differentiated segment coordinates the stepwise recruitment of undifferentiated cells from the segment/pygidium boundary. 相似文献
143.
144.
Andra Rizqiawan Kei TobiumeGaku Okui Kazuhiro YamamotoHideo Shigeishi Shigehiro OnoHiroshi Shimasue Masaaki TakechiKoichiro Higashikawa Nobuyuki Kamata 《Biochemical and biophysical research communications》2013
We found that high galectin-1 (Gal-1) mRNA levels were associated with invasive squamous cell carcinoma (SCC) cells that expressed Snail, an epithelial-to-mesenchymal transition (EMT) regulator. Both Gal-1 overexpression and soluble Gal-1 treatment accelerated invasion and collective cell migration, along with activation of cdc42 and Rac. Soluble Gal-1 activated c-Jun N-terminal kinase to increase expression levels of integrins α2 and β5, which were essential for Gal-1 dependent collective cell migration and invasiveness. Soluble Gal-1 also increased the incidence of EMT in Snail-expressing SCC cells; these were a minor population with an EMT phenotype under growing conditions. Our findings indicate that soluble Gal-1 promotes invasiveness through enhancing collective cell migration and increasing the incidence of EMT. 相似文献
145.
Sayuri Ishiwata Shigehiro Ogata Asami Umino Hirohisa Shiraku Yoshitaka Ohashi Yasushi Kajii Toru Nishikawa 《Amino acids》2013,44(5):1391-1395
In an in vivo dialysis experiment, the intra-medial frontal cortex infusion of a system A and Asc-1 transporter inhibitor, S-methyl-l-cysteine, caused a concentration-dependent increase in the dialysate contents of an endogenous coagonist for the N-methyl-d-aspartate (NMDA) type glutamate receptor, d-serine, in the cortical portion. These results suggest that these neutral amino acid transporters could control the extracellular d-serine signaling in the brain and be a target for the development of a novel threapy for neuropsychiatric disorders with an NMDA receptor dysfunction. 相似文献
146.
Konoshin Onodera Eiichi Maekawa Shozaburo Kitaoka Shigehiro Hirano 《Bioscience, biotechnology, and biochemistry》2013,77(1):60-64
N-Acetyl-D-galactosamine, N-acetyl-D-mannosamine and N-acetyl-D-glucosamine were allowed to react with oxalacetic acid under alkaline conditions, and the condensation products purified by ion-exchange chromatography. Properties of these products on the whole are similar to each other, though there is a minor but significant diference in the condensation product with N-acetyl-D-galactosaminc. Paper chromatograms of the condensation products suggest that N-acetyl-D-galactosamine as well as N-acetyl-D-glucosamine are epimerized partly before they condense with oxalacetic acid to givc each two sialic acids with different configurations at C-5 from each other. 相似文献
147.
The methylations of heparin, heparitinsulfate, and three oligosaccharides (hexa, tetra and trisaccharides) from hyaluronic acid were carried out. Theoretical amounts of methoxyl group were obtained from the oligosaccharides and the desulfated product of carboxyl-reduced heparitinsulfate, but not from heparin and heparitinsulfate. The acid hydrolysis of the methylated trisaccharide confirms that the structure is β-d-glucopyranosyl uronic acid-(1→3) -2-acetamido-2-deoxy-β-d-glucopyranosyl- (1→4) -d-glucopyranosyl uronic acid. 相似文献
148.
Yoshiyuki Inouye Tatsuro Ito Takeyoshi Nakahara Akihiko Fukuda Shigehiro Hirano Isao Morishima 《Bioscience, biotechnology, and biochemistry》2013,77(8):1415-1421
Two carbohydrate rich fractions A and B were isolated from wheat gluten. Fraction B contained more lipid than fraction A. Lipid portion of fraction B consisted mainly of glycolipid and was fractionated into five fractions by thin-layer chromatography. The two main fractions were extracted and determined to be galactolipid and glucolipid, respectively, by the analyses of fatty acid and sugar components by gas chromatography. Defatted fraction A was assumed to consist of glycoprotein. After complete pronase digestion of defatted fraction A, the remaining glycopeptide moiety was isolated by column chromatography on DEAE-cellulose followed by gel filtration through Sephadex G–25. The amino acid and sugar components of the glycopeptide were investigated. 相似文献
149.
150.
Komiya M Asano S Koike N Koga E Igarashi J Nakatani S Isobe Y 《Bioorganic & medicinal chemistry letters》2011,21(5):1456-1458
A series of novel 2-substituted-5-hydroxyindoles were synthesized and evaluated for their inhibitory activity against CaMKII. Structure and activity relationship results indicated that potent inhibitory activity could be achieved by modification at the para-position of the phenyl ring of the high throughput screening hit compound 2. Among the prepared compounds, we identified 14 as a novel CaMKII inhibitor with an activity stronger than that of KN-93, a known CaMKII inhibitor. 相似文献