全文获取类型
收费全文 | 76267篇 |
免费 | 5580篇 |
国内免费 | 4781篇 |
出版年
2024年 | 154篇 |
2023年 | 1036篇 |
2022年 | 2394篇 |
2021年 | 4080篇 |
2020年 | 2622篇 |
2019年 | 3241篇 |
2018年 | 3173篇 |
2017年 | 2302篇 |
2016年 | 3257篇 |
2015年 | 4815篇 |
2014年 | 5548篇 |
2013年 | 5992篇 |
2012年 | 7023篇 |
2011年 | 6158篇 |
2010年 | 3711篇 |
2009年 | 3335篇 |
2008年 | 3726篇 |
2007年 | 3354篇 |
2006年 | 2906篇 |
2005年 | 2380篇 |
2004年 | 1957篇 |
2003年 | 1653篇 |
2002年 | 1399篇 |
2001年 | 1230篇 |
2000年 | 1218篇 |
1999年 | 1121篇 |
1998年 | 661篇 |
1997年 | 655篇 |
1996年 | 667篇 |
1995年 | 616篇 |
1994年 | 543篇 |
1993年 | 376篇 |
1992年 | 568篇 |
1991年 | 435篇 |
1990年 | 406篇 |
1989年 | 282篇 |
1988年 | 244篇 |
1987年 | 234篇 |
1986年 | 166篇 |
1985年 | 193篇 |
1984年 | 109篇 |
1983年 | 117篇 |
1982年 | 71篇 |
1981年 | 58篇 |
1980年 | 37篇 |
1979年 | 61篇 |
1977年 | 30篇 |
1974年 | 38篇 |
1973年 | 34篇 |
1972年 | 30篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
171.
E J Gibbs M C Maurer J H Zhang W M Reiff D T Hill M Malicka-Blaszkiewicz R E McKinnie H Q Liu R F Pasternack 《Journal of inorganic biochemistry》1988,32(1):39-65
Studies of the solution properties of gold(III)tetrakis(4-N-methylpyridyl) porphine and its DNA binding characteristics have been conducted utilizing uv/vis absorption spectroscopy, circular dichroism (CD), Mossbauer spectroscopy, and temperature-jump relaxation techniques. These studies indicate that over the concentration range considered this water soluble gold(III) porphyrin does not aggregate, binds axial ligands only weakly with a preference for soft Lewis bases, and is capable of intercalation into nucleic acids of appropriate base pair content. The interaction of this and several other porphyrins with the synthetic polynucleotide poly(dA-dC).poly(dT-dG) has been studied. Spectroscopic signatures for intercalation were found for those derivatives not having axial ligands. Intercalation into chromatin in vitro can also occur with those porphyrins and metalloporphyrins which do not have axial ligands. Finally, studies utilizing microinjection techniques indicate that once within the cell, tetrakis(4-N-methylpyridyl)porphine tends to localize in the nucleus. 相似文献
172.
173.
Sera from both human cytomegalovirus (HCMV)-seropositive adults and infants with congenital HCMV infection recognized two major HCMV glycoprotein complexes. However, proliferative responses of peripheral blood mononuclear cells to these complexes varied among seropositive adults and were not detected in any of the infants. Thus, these glycoproteins alone may not be sufficient to develop a subviral HCMV vaccine. 相似文献
174.
Chromosome breakage caused by mutagens in male germ cells can be analyzed by micronucleus induction during meiotic division. This can be followed in vitro by culturing seminiferous tubular segments from stages of the epithelial cycle that contain late pachytene and diakinetic primary spermatocytes. We studied the mutagenic potential of a male contraceptive, gossypol, in this test system using adriamycin (10 ng/ml) as a reference mutagen. A small but significant increase in the frequency of micronuclei was induced with concentrations of 10 and 20 micrograms/ml of gossypol, while cytotoxic effects appeared at concentration of 20 micrograms/ml and were evident at 50 micrograms/ml. Analysis of meiotic micronucleus induction in vitro seems to be a sensitive test system of male germ-cell mutagenesis, but further studies on the possible mutagenic effects of gossypol are needed. 相似文献
175.
Applied anatomy of the anterolateral femoral flap 总被引:13,自引:0,他引:13
D C Xu S Z Zhong J M Kong G Y Wang M Z Liu L S Luo J H Gao 《Plastic and reconstructive surgery》1988,82(2):305-310
A study of the source of the blood supply to the anterolateral femoral flap was carried out on 42 lower limbs of adult cadavers (among them 35 cadavers with injection of red latex and 1 with india ink into the arteries and 6 vascular cast specimens), and the surface locations of the vascular pedicle were detected on 50 healthy adults. It was found that the descending branch of the lateral circumflex femoral vessel was an ideal axial vessel. There are constant perforating branches of the myocutaneous artery or cutaneous branches from the intermuscular space to the anterolateral femoral skin. The area extends about 12 x 30 cm. Within the flap, the anterior branch of the anterolateral cutaneous nerve of the high is located. This flap has been widely used for free transplantation in China since 1983 with satisfactory results. 相似文献
176.
177.
我国沙漠中部地区主要不同生态类型植物脯氨酸的累积、光合、呼吸和叶绿素含量 总被引:21,自引:0,他引:21
中生植物脯氨酸含量为0.42mg/g.dw, 低于少浆旱生植物(1.73mg/g.dw)。多浆旱生植物脯氨酸含量最高(7.22mg/g.dw),为前两者的17倍和4倍,两类旱生植物在干旱条件下(非灌溉)脯氨酸含量均高于灌水处理。少浆与多浆旱生植物的光合强度(16.74,14.04CO2mg/g.dw.h)差异不大,而中生植物(37.57mg/g.dW.h)略高于多浆旱生植物(4.73CO2Mg/g.dw.h),与中生植物(7.60Co2mg/g.dw.h)接近,光合/呼吸值,少浆,多浆与中生植物分别为2.50,3.09和4.59,说明中生植物的合成明显大于消耗,季节动态中,中生植物显著高于两类旱生植物,叶绿素总量三类植物差异甚微。 相似文献
178.
除虫菊的染色体数目及其核型 总被引:6,自引:0,他引:6
杜冰群;刘启宏;朱翠英 《武汉植物学研究》1988,6(1):95-96
除虫菊(Pyrethrum cinerariifolium Trev.)为菊科小黄菊属多年生宿根草本植物。以花或全草入药。除虫菊的主要有效成分为除虫菊酯和瓜叶除虫菊酯,可用于加工成各种制剂作杀虫剂原料,用以防治日常虫豸(蚊、蝇、虱等)和农业害虫,且对人 相似文献
179.
180.