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131.
132.
Phenotypic plasticity is the environmental modification of genotypic expression
and an important means by which individual plants respond to environmental heterogeneity.
The study of phenotypic plasticity in the genus Adenophora, which is very complicated taxo nomically because of great morphological variation, proves to be helpful in both investigating
the phenotypic variation so as to evaluate potential taxonomic value of their characters and
providing important sources of information on the variation, adaptation and evolution of the
genus. Twenty-three populations representing all the six species in Adenophora potaninii
complex were transplanted into the garden. Of them six populations were selected for study ing their performance in the field and in the garden, in addition to cultivation experiment
under different treatments. The results show that there exists considerable developmental
plasticity in some leaf, floral and capsule characters. In particular, the leaf shape and length
of calyx lobe display significant developmental variation with the maximum being three times
as great as the minimum, which is noteworthy because they were previously considered as
diagnostic. The characters of root, caudex, stem and inflorescence are found to be very
plastic, especially the root diameter, the number of stems, stem height and inflorescence
length with great environmental plasticity. In addition, the populations from different habi tats show distinct amounts of plasticity. On the contrary, the characters of leaf, floral, cap sule and seed are less influenced by environments. It seems that the considerable variation in
the characters of leaf is attributed mainly to genetic differences. Finally, the phenotypic
plasticity of morphological characters of A. potaninii complex and its taxonomic significanceis discussed. 相似文献
133.
地上部植食者褐飞虱对不同水稻品种土壤线虫群落的影响 总被引:2,自引:0,他引:2
地上和地下部生物群落的交互作用对于调控陆地生态过程具有重要作用。在盆栽条件下利用2×2析因设计研究了褐飞虱(Nilaparvata lugens)取食不同水稻品种后对土壤线虫群落的影响。结果表明, 褐飞虱侵害水稻9 d后, 感虫品种(广四和汕优63)的土壤线虫总数、属数及自生线虫(食细菌线虫、食真菌线虫和捕食性线虫)数量增加, 并且一般达到显著水平(P<0.05); 而上述指标在抗虫品种(汕优559和IR36)土壤中则呈现相反的趋势。植食性线虫数量在强感虫品种广四上显著增加(P<0.05), 而在强抗虫品种IR36上显著减少(P<0.05)。褐飞虱和水稻品种对土壤线虫的生态指数(线虫通道指数、Shannon-Wiener指数、成熟度指数、富集指数和结构指数)没有明显影响, 可能与供试土壤线虫群落组成单一及褐飞虱作用时间较短有关。总之, 褐飞虱强烈影响土壤线虫数量、群落组成和营养结构, 并且作用的方向(促进或抑制)和程度依赖于水稻的品种特性, 揭示出地上部植食者的短期侵害将对稻田土壤生态系统的结构和功能产生深远影响。 相似文献
134.
135.
The metabolic stability of peptides containing a mixed sequence of α-aminoxy acids and α-amino acids is significantly improved compared to peptides composed of only natural α-amino acids. The introduction of an α-aminoxy acid into peptide chain dramatically improves the stability of the amide bonds immediately before and after it. These peptides containing α-aminoxy acids represent excellent structural scaffold for the design of metabolically stable and biologically active peptides. 相似文献
136.
137.
Shuqing Ge Tao Li Qijian Yao Hongling Yan Zhang Huiyun Yanshan Zheng Bin Zhang Shaoheng He 《Cell biology and toxicology》2016,32(6):529-542
Serine proteases play an important role in inflammation via PARs. However, little is known of expression levels of PARs on monocytes of allergic patients, and influence of serine proteases and PARs on TNF-α secretion from monocytes. Using quantitative real-time PCR (qPCR) and flowcytometry techniques, we observed that the expression level of PAR-2 in monocytes of patients with allergic rhinitis and asthma was increased by 42.9 and 38.2 %. It was found that trypsin, thrombin, and tryptase induced up to 200, 320, and 310 % increase in TNF-α release from monocytes at 16 h, respectively. PAR-1 agonist peptide, SFLLR-NH2, and PAR-2 agonist peptide tc-LIGRLO-NH2 provoked up to 210 and 240 % increase in release of TNF-α. Since SCH 79797, a PAR-1 antagonist, and PD98059, an inhibitor of ERK inhibited thrombin- and SFLLR-NH2-induced TNF-α release, the action of thrombin is most likely through a PAR-1- and ERK-mediated signaling mechanism. Similarly, because FSLLRN-NH2, an inhibitor of PAR-2 diminished tryptase- and tc-LIGRLO-NH2-induced TNF-α release, the action of tryptase appears PAR-2 dependent. Moreover, in vivo study showed that both recombinant cockroach major allergens Per a 1 and Per a 7 provoked upregulation of PAR-2 and PAR-1 expression on CD14+ cells in OVA-sensitized mouse peritoneum. In conclusion, increased expression of PAR-2 in monocytes of AR and asthma implicates that PAR-2 likely play a role in allergy. PAR-2- and PAR-1-mediated TNF-α release from monocytes suggests that these unique protease receptors are involved in the pathogenesis of inflammation. 相似文献
138.
Tyrosine phosphorylation of mitochondrial pyruvate dehydrogenase kinase 1 is important for cancer metabolism 总被引:1,自引:0,他引:1
Hitosugi T Fan J Chung TW Lythgoe K Wang X Xie J Ge Q Gu TL Polakiewicz RD Roesel JL Chen GZ Boggon TJ Lonial S Fu H Khuri FR Kang S Chen J 《Molecular cell》2011,44(6):864-877
Many tumor cells rely on aerobic glycolysis instead of oxidative phosphorylation for their continued proliferation and survival. Myc and HIF-1 are believed to promote such a metabolic switch by, in part, upregulating gene expression of pyruvate dehydrogenase (PDH) kinase 1 (PDHK1), which phosphorylates and inactivates mitochondrial PDH and consequently pyruvate dehydrogenase complex (PDC). Here we report that tyrosine phosphorylation enhances PDHK1 kinase activity by promoting ATP and PDC binding. Functional PDC can form in mitochondria outside of the matrix in some cancer cells and PDHK1 is commonly tyrosine phosphorylated in human cancers by diverse oncogenic tyrosine kinases localized to different mitochondrial compartments. Expression of phosphorylation-deficient, catalytic hypomorph PDHK1 mutants in cancer cells leads to decreased cell proliferation under hypoxia and increased oxidative phosphorylation with enhanced mitochondrial utilization of pyruvate and reduced tumor growth in xenograft nude mice. Together, tyrosine phosphorylation activates PDHK1 to promote the Warburg effect and tumor growth. 相似文献
139.
以4个不同烤烟品种为研究材料,采用盆栽试验,运用高效毛细管区带电泳法测定各品种根际土、非根际土、根系及叶片中的单糖组分及含量,并分析其相关关系,探究根系分泌物中糖类的分泌特性。结果表明:在各样品中,共检出木糖、葡萄糖、半乳糖、核糖、阿拉伯糖和鼠李糖6种糖;不同品种根际土、非根际土、根系及叶片中检出的糖组分及含量均存在差异;同一品种中,叶片最高,根系次之,根际土和非根际土最低;相关性分析表明,木糖、阿拉伯糖、葡萄糖、鼠李糖和半乳糖总量在根际土、非根际土、叶片和根系间呈正相关关系,各单糖组分间均呈正相关关系,部分组分呈显著或极显著相关关系。研究表明,不同烤烟品种根系分泌这些单糖存在品种差异,且根系分泌单糖可能是一个沿浓度梯度的扩散过程。 相似文献
140.
The East Himalaya-Hengduan Mountains region is the center of diversity of the genus Primula, and P. sikkimensis is one of the most common members of the genus in the region. In this study, the genetic diversity and structure of P. sikkimensis populations in China were assessed using inter-simple sequence repeat (ISSR) and chloroplast microsatellite markers. The
254 individuals analyzed represented 13 populations. High levels of genetic diversity were revealed by ISSR markers. At the
species level, the expected heterozygosity and Shannon’s index were 0.4032 and 0.5576, respectively. AMOVA analysis showed
that 50.3% of the total genetic diversity was partitioned among populations. Three pairs of chloroplast microsatellite primers
tested yielded a total of 12 size variants and 15 chloroplast haplotypes. Strong cpDNA genetic differentiation (G
ST = 0.697) and evidence for phylogeographic structure were detected (N
ST = 0.788, significantly higher than G
ST). Estimated rates of pollen-mediated gene flow are approximately 27% greater than estimated rates of seed-mediated gene flow
in P. sikkimensis. Both seed and pollen dispersal, however, are limited, and gene flow among populations appears to be hindered by the patchiness
of the species’ habitats and their geographic isolation. These features may have played important roles in shaping the genetic
structure of P. sikkimensis. A minimum-spanning tree of chloroplast DNA haplotypes was constructed, and possible glacial refugia of P. sikkimensis were identified. 相似文献