全文获取类型
收费全文 | 23334篇 |
免费 | 1802篇 |
国内免费 | 1915篇 |
专业分类
27051篇 |
出版年
2024年 | 68篇 |
2023年 | 331篇 |
2022年 | 797篇 |
2021年 | 1375篇 |
2020年 | 838篇 |
2019年 | 1004篇 |
2018年 | 1033篇 |
2017年 | 827篇 |
2016年 | 1003篇 |
2015年 | 1438篇 |
2014年 | 1697篇 |
2013年 | 1855篇 |
2012年 | 2182篇 |
2011年 | 1944篇 |
2010年 | 1200篇 |
2009年 | 1050篇 |
2008年 | 1158篇 |
2007年 | 982篇 |
2006年 | 903篇 |
2005年 | 736篇 |
2004年 | 637篇 |
2003年 | 505篇 |
2002年 | 491篇 |
2001年 | 417篇 |
2000年 | 363篇 |
1999年 | 353篇 |
1998年 | 248篇 |
1997年 | 214篇 |
1996年 | 188篇 |
1995年 | 181篇 |
1994年 | 207篇 |
1993年 | 126篇 |
1992年 | 133篇 |
1991年 | 103篇 |
1990年 | 91篇 |
1989年 | 89篇 |
1988年 | 72篇 |
1987年 | 45篇 |
1986年 | 43篇 |
1985年 | 39篇 |
1984年 | 29篇 |
1983年 | 20篇 |
1982年 | 14篇 |
1981年 | 3篇 |
1980年 | 7篇 |
1979年 | 5篇 |
1978年 | 2篇 |
1975年 | 2篇 |
1950年 | 1篇 |
1933年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
981.
982.
Cheng Peng Qing Wang Herong Shi Jiaxing Chen Shuisheng Li Huihong Zhao Haoran Lin Jianchun Yang Yong Zhang 《Journal of fish biology》2020,97(3):785-793
Sexual patterns of teleosts are extremely diverse and include both gonochorism and hermaphroditism. As a protogynous hermaphroditic fish, all orange-spotted groupers (Epinephelus coioides) develop directly into females, and some individuals change sex to become functional males later in life. This study investigated gonadal restructuring, shifts in sex hormone levels and gene profiles of cultured mature female groupers during the first (main) breeding season of 2019 in Huizhou, China (22° 42′ 02.6″ N, 114° 32′ 10.1″ E). Analysis of gonadal restructuring revealed that females with pre-vitellogenic ovaries underwent vitellogenesis, spawning and regression and then returned to the pre-vitellogenic stage in the late breeding season, at which point some changed sex to become males via the intersex gonad stage. A significant decrease in the level of serum 17β-estradiol (E2) was observed during ovary regression but not during sex change, whereas serum 11-ketotestosterone (11-KT) concentrations increased significantly during sex change with the highest concentration in newly developed males. Consistent with serum hormone changes, a significant decrease in cyp19a1a expression was observed during ovary regression but not during sex change, whereas the expression of cyp11c1 and hsd11b2 increased significantly during sex change. Interestingly, hsd11b2 but not cyp11c1 was significantly upregulated from the pre-vitellogenic ovary stage to the early intersex gonad stage. These results suggest that a decrease in serum E2 concentration and downregulation of cyp19a1a expression are not necessary to trigger the female-to-male transformation, whereas increased 11-KT concentration and upregulation of hsd11b2 expression may be key events for the initiation of sex change in the orange-spotted grouper. 相似文献
983.
Qi Li Ji Wang Tian Bai Ming Zhang Yuling Jia Danyu Shen Meixiang Zhang Daolong Dou 《Molecular Plant Pathology》2020,21(4):502-511
EDS1 (Enhanced Disease Susceptibility 1) plays a crucial role in both effector-triggered immunity activation and plant basal defence. However, whether pathogen effectors can target EDS1 or an EDS1-related pathway to manipulate immunity is rarely reported. In this study, we identified a Phytophthora capsici Avirulence Homolog (Avh) RxLR (Arg-any amino acid-Leu-Arg) effector PcAvh103 that interacts with EDS1. We demonstrated that PcAvh103 can facilitate P. capsici infection and is required for pathogen virulence. Furthermore, genetic evidence showed that PcAvh103 contributes to virulence through targeting EDS1. Finally, PcAvh103 specifically interacts with the lipase domain of EDS1 and can promote the disassociation of EDS1–PAD4 (Phytoalexin Deficient 4) complex in planta. Together, our results revealed that the P. capsici RxLR effector PcAvh103 targets host EDS1 to suppress plant immunity, probably through disrupting the EDS1–PAD4 immune signalling pathway. 相似文献
984.
EPA plays multiple roles in regulating lipid accumulation of grass carp Ctenopharyngodon idella adipose tissue in vitro and in vivo 下载免费PDF全文
This study was conducted to assess the effect of eicosapentaenoic acid (20:5n‐3, EPA) on lipid accumulation in grass carp Ctenopharyngodon idella adipose tissue both in vitro and in vivo. EPA was observed to inhibit the adipocyte viability in a time and dose‐dependent manner. EPA was also found to induce reactive oxygen species accumulation in vitro. The mRNA levels of caspase 3a and caspase 3b, as well as the activity of Caspase 3 increased significantly in vitro and in vivo, whereas the value of B cell leukemia 2–Bcl‐2 associated X protein decreased significantly. Besides, the pro‐apoptotic effect was relieved by α‐tocopherol. Dietary 0.52% EPA had no apparent effect on intraperitoneal fat index. Moreover, EPA promoted the hydrolytic gene expressions in vitro and in vivo, including adipose triglyceride lipase and hormone sensitive lipase‐a. Meanwhile, the lipogenic gene expressions of liver X receptor α, sterol regulatory element binding protein‐1c and fatty‐acid synthase were down‐regulated by EPA in vitro and in vivo. However, EPA also acted to promote the marker gene expressions of adipogenesis, including peroxisome proliferator‐activated receptor γ and lipoprotein lipase in vitro and in vivo. Contents of EPA increased significantly in the treatment groups in vitro and in vivo. These results support that EPA affects multiple aspects of lipid metabolism, including hydrolysis, lipogenesis, adipogenesis and apoptosis. However, it barely functioned in decreasing the lipid accumulation of Ctenopharyngodon idella under the current culture conditions. 相似文献
985.
986.
987.
988.
989.
Conall Sauvey Gretchen Ehrenkaufer Da Shi Anjan Debnath Ruben Abagyan 《PLoS neglected tropical diseases》2021,15(2)
Entamoeba histolytica is a protozoan parasite which infects approximately 50 million people worldwide, resulting in an estimated 70,000 deaths every year. Since the 1960s E. histolytica infection has been successfully treated with metronidazole. However, drawbacks to metronidazole therapy exist, including adverse effects, a long treatment course, and the need for an additional drug to prevent cyst-mediated transmission. E. histolytica possesses a kinome with approximately 300–400 members, some of which have been previously studied as potential targets for the development of amoebicidal drug candidates. However, while these efforts have uncovered novel potent inhibitors of E. histolytica kinases, none have resulted in approved drugs. In this study we took the alternative approach of testing a set of twelve previously FDA-approved antineoplastic kinase inhibitors against E. histolytica trophozoites in vitro. This resulted in the identification of dasatinib, bosutinib, and ibrutinib as amoebicidal agents at low-micromolar concentrations. Next, we utilized a recently developed computational tool to identify twelve additional drugs with human protein target profiles similar to the three initial hits. Testing of these additional twelve drugs led to the identification of ponatinib, neratinib, and olmutinib were identified as highly potent, with EC50 values in the sub-micromolar range. All of these six drugs were found to kill E. histolytica trophozoites as rapidly as metronidazole. Furthermore, ibrutinib was found to kill the transmissible cyst stage of the model organism E. invadens. Ibrutinib thus possesses both amoebicidal and cysticidal properties, in contrast to all drugs used in the current therapeutic strategy. These findings together reveal antineoplastic kinase inhibitors as a highly promising class of potent drugs against this widespread and devastating disease. 相似文献
990.
Rui Liu Ting Zhu Tao Yang Zhengyan Yang Ang Ren Liang Shi Jing Zhu Hanshou Yu Mingwen Zhao 《Environmental microbiology》2021,23(2):682-695
Nitric oxide (NO) is an important signalling molecule in stress response of organisms. We previously reported that NO decreases heat stress (HS)-induced ganoderic acid (GA) accumulation in Ganoderma lucidum. To explore the mechanisms by which NO modulates GA biosynthesis under HS, the effect of NO on the reactive oxygen species (ROS) content was examined. The results showed that NO decreased the production of mitochondrial ROS (mitROS) by 60% under HS. Further research revealed that NO reduced the mitROS content by inhibiting aconitase (Acon) activity. The GA content in Acon-silenced (Aconi) strains treated with NO donor did not differ significantly from that in untreated Aconi strains. To study the mechanism by which Acon activity is inhibited, the S-nitrosylation level of Acon was determined. Biotin-switch technology and mass spectrometry analysis were used to show that Acon is S-nitrosylated at the Cys-594 amino acid residue. Substitution of Cys-594 with a Ser, which cannot be S-nitrosylated, abolished the responsiveness of Acon to the NO-induced reduction in its enzymatic activity. These findings demonstrate that NO inhibits Acon activity through S-nitrosylation at Cys-594. In summary, these findings describe mechanism by which NO regulates GA biosynthesis via S-nitrosylation of Acon under HS condition in G. lucidum. 相似文献