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121.
Sherwin Carlquist 《American journal of botany》1982,69(10):1587-1598
Quantitative and qualitative features are reported for 23 collections of ten species of Illicium, sole genus of Illiciaceae. Vessel elements are long, thin-walled, and angular; perforation plates are scalariform and range from long to moderately long; vessel-ray pitting is scalariform to opposite. Tracheids bear fully bordered pits. Axial parenchyma is sparse, abaxial to vessels with some diffuse cells also present. Rays are both multiseriate and uniseriate; the former are lacking near the pith in some species. No erect sheathing cells are present on multiseriate rays, and procumbent cells become more abundant as stems increase in size. Four species from montane subequatorial highlands lack growth rings and helical sculpture in vessels; the remainder of Illicium species have these features. Notably narrow vessels and large numbers of vessels per sq. mm characterize the temperate species from northern limits for the genus, I. anisatum, I. floridanum, and I. parviflorum. Greater vessel density offers redundancy and greater safety and is correlated with greater frost and possibly greater fluctuation in moisture availability. These two ecological features are probably also related to the narrow vessel diameter, which may retard entry of air embolisms (lowered air entry values) in accord with the physiological considerations of Slatyer. Reduction of number of bars per perforation plate within Illicium is also correlated with the more northerly climates. An additional hypothesis for evolution of shorter vessel elements in dicotyledons is introduced: if, as claimed by Slatyer, air embolisms in vessels tend to stop at ends of vessel elements and thus do not disable entire vessels, shorter vessel elements would maximally localize air embolisms. Presence of helical sculpture and other forms of relief within vessels has been difficult to explain in conifers and dicotyledons; such features might help resist cavitations by increasing adhesion of water molecules to cell walls (hydration). 相似文献
122.
June Almenoff Sherwin Wilk Marian Orlowski 《Biochemical and biophysical research communications》1981,102(1):206-214
A membrane bound zinc-metalloendopeptidase from bovine pituitaries with a specificity toward bonds on the amino side of hydrophobic amino acids, cleaves Met- and Leu-enkephalin at the Gly-Phe bond, releasing Phe-Met and Phe-Leu respectively. The enzyme also hydrolyzes bonds on the amino side of hydrophobic amino acids in oxytocin, bradykinin, neurotensin and several synthetic substrates. A free carboxyl group on a dipeptide C-terminal to the hydrolyzed bond is not a requirement for activity. The enzyme is also present in brain membrane fractions. The regional distribution of this enzyme in brain, its specificity toward natural and synthetic substrates, and its sensitivity to inhibitors, suggest that the enzyme is identical to an activity referred to as “enkephalinase”, which has been described as dipeptidyl carboxypeptidase. The data show that the enzyme is an endopeptidase with a specificity similar to that of a group of microbial proteases, one of which is thermolysin. 相似文献
123.
Marian Orlowski Charlene Michaud Sherwin Wilk 《Biochemical and biophysical research communications》1980,94(4):1145-1153
Highly purified preparations of cation-sensitive neutral endopeptidase, from bovine pituitary, and also rabbit brain, generate methionine-enkephalin, from α-endorphin, a peptide containing the amino acid sequence 61–76 of β-lipotropin (β-LPH), The enzyme also catalyzes the hydrolysis of the Leu-Thr bond in the synthetic peptide Tyr-Gly-Gly-Phe-Leu-Thr-2-naphthylamide with the release of leucine-enkephalin and Thr-2-naphthylamide. Neither Met- nor Leu-enkephalin are degraded. The data indicate that the presence of a free N-terminal group of tyrosine inhibits the further degradation of Leu- and Met-enkephalin by the endopeptidase. It is suggested that cation-sensitive neutral endopeptidase is one of the enzymes capable of generating Met- and Leu-enkephalin . 相似文献
124.
Testosterone and sexual behavior in oral contraceptive users and nonusers: a prospective study 总被引:2,自引:1,他引:1
The relationship between plasma testosterone (T) secretion and patterns of sexual behavior was examined in 18 women using oral contraceptives (OCs) and 13 nonusers matched for partner availability. Retrospective assessments of perimenstrual symptoms, sexual attitudes, and sexual experience were obtained and women completed daily ratings of the frequency of sexual activities and the level of well-being for 1 month. Plasma levels of sex hormone binding globulin (SHBG), progesterone, Total T, Free T, and non-SHBG bound T were determined by radioimmunoassay at four phases of the pill or menstrual cycle. Overall, women not using OCs had higher plasma levels of Total, Free, and non-SHBG bound T and lower plasma levels of SHBG than those of OC users. Further, only nonusers had perimenstrual decreases in plasma levels of Total and Free T. The two groups were comparable on most retrospective measures. However, OC users reported more satisfaction with their sexual partners than did nonusers and prospective monitoring revealed that they engaged in sexual interactions more frequently than did nonusers across the cycle. In contrast, both groups reported a similar frequency of autosexual activities across the cycle. There were no correlations between average levels of T and levels of sexual desire, sexual interactions, or autosexuality. Moreover, only nonusers reported a decrease in levels of sexual desire during the perimenstrual period that was associated with the changes in Free T over the menstrual cycle. 相似文献
125.
Rescue of endogenous 30S retroviral sequences from mouse cells by baboon type C virus. 总被引:25,自引:16,他引:9
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Mus musculus SC-1 cells were infected with M7 baboon type C virus. The progeny of this infection included viral pseudotypes that contained M7 helper virus and endogenous 30S retrovirus-associated sequences derived from SC-1 cells (RAS). The RAS sequences are unrelated by nucleic acid hybridization criteria to previously described types of murine retroviruses and do not code for known murine viral structural proteins. The RAS genome is present in multiple copies in the DNA of laboratory (M. musculus) and Asian (M. caroli and M. cervicolor) mice, is expressed in the RNA of uninfected mouse cells, and can be efficiently rescued by type C, but not type B, viruses. RAS is closely related to 30S virus-associated RNA in NIH/3T3 and BALB/c JLSV-9 cells and may be analogous to the defective 30S RNA sequences found in rats. 相似文献
126.
SEM studies of roots and rhizomes of Triglochin (one species) and Maundia (monotypic) of Juncaginaceae and the sole species of Scheuchzeriaceae, Scheuchzeria palustris, reveals that vessels are present not only in roots, as previously reported, but also in rhizomes. The perforations contain pit membranes with pores of various sizes. Striate pit membranes, like those previously seen in Acorus, occur on pit remnants in peforations and on pit membranes of lateral walls in all genera studied. Grooves interconnecting pit apertures are illustrated for root tracheary elements of Triglochin; this is believed to be a first report of this feature for monocotyledons. The tracheary elements of Juncaginaceae and Scheuchzeriaceae are similar in their thick walls and narrow slitlike pits, lending support to the close relationship between the two families often claimed. 相似文献
127.
Scanning electron microscope (SEM) studies of paraffin-sectioned material of stems and roots of Barclaya rotundifolia Hotta revealed perforations on tracheary elements of roots, but not on those of stems. End walls of vessels are identical with lateral walls except for the presence of perforations. Perforations can only be clearly revealed with SEM, and this method is advocated for further study of tracheary elements of Nymphaeaceae in particular, and primary xylem of ∗∗∗angiosperms in general. Vessel presence may be related to the habitat of this species, which unlike other members of the Nymphaeaceae (sensu stricto) has only aerial leaves and a rhizomatous system that is not inundated for prolonged periods. 相似文献
128.
PRESENCE OF VESSELS IN WOOD OF SARCANDRA (CHLORANTHACEAE); COMMENTS ON VESSEL ORIGINS IN ANGIOSPERMS
Sherwin Carlquist 《American journal of botany》1987,74(12):1765-1771
Sarcandra is the only genus of Chloranthaceae hitherto thought to be vesselless. Study of liquid-preserved material of S. glabra revealed that in root secondary xylem some tracheary elements are wider in diameter and have markedly scalariform end walls combined with circular pits on lateral walls. Examination of these wider tracheary elements with scanning electron microscope (SEM) demonstrated various degrees of pit membrane absence in the end walls. Commonly a few threadlike fibrils traverse the pits (perforations); these as well as intact nature of pit membranes in pits at ends of some perforation plates are evidence that lack of pit membranes does not result from damage during processing. Some perforations lack any remnants of pit membranes. Although perforation plates and therefore vessels are present in Sarcandra roots, no perforations were observed in tracheary elements of stems or lignotubers. Further, stem tracheids do not have the prominently scalariform end walls that the vessel elements in roots do. Presence of vessels in Sarcandra removes at least one (probably several) hypothetical events of vessel origin that must be postulated to account for known patterns of vessel distribution in angiosperms, assuming that they are primitively vesselless. Seven (perhaps fewer) vessel origin events in angiosperms could account for these patterns; two of those events (Nelumbo and monocotyledons) are different from the others in nature. Widely accepted data on trends of vessel specialization in woody dicotyledons yield an unappreciated implication: vessel specialization has happened in a highly polyphyletic manner in dicotyledons, and therefore multiple vessel origins represent a logical extension backward in time. If a group of vesselless dictyoledons ancestral to other angiosperms existed, they can be hypothesized to have had a relatively homogeneous floral plan now that Sarcandra-like plants no longer need be imagined within that group. Sarcandra and other Chloranthaceae show that the borderline between vessel absence and presence is less sharp than generally appreciated. 相似文献
129.
Comparison of the Effect of Calpain Inhibitors on Two Extralysosomal Proteinases: The Multicatalytic Proteinase Complex and m-Calpain 总被引:7,自引:0,他引:7
Maria E. Figueiredo-Pereira Naren Banik Sherwin Wilk 《Journal of neurochemistry》1994,62(5):1989-1994
Abstract: The potencies of three peptide aldehyde inhibitors of calpain (calpain inhibitors 1 and 2 and calpeptin) as inhibitors of four catalytic activities of the multicatalytic proteinase complex (MPC) were compared with their potencies as inhibitors of m-calpain. The chymotrypsinlike activity (cleavage after hydrophobic amino acids) and the caseinolytic activity (degradation of β-casein) of MPC were strongly inhibited by calpain inhibitors 1 and 2 (IC50 values in the low micromolar range). Cleavage by MPC after acidic amino acids (peptidylglutamyl-peptide bond hydrolyzing activity) and basic amino acids (trypsinlike activity) was inhibited less effectively, declining moderately with increasing concentrations of calpain inhibitors 1 and 2. Calpeptin only weakly inhibited the four MPC activities, yet was the most potent inhibitor of m-calpain. These results indicate that caution must be exercised when calpain inhibitors 1 and 2 are used to infer calpain function. Calpeptin may be a better choice for such studies, although its effect on other cysteine or serine proteinases remains to be determined. 相似文献
130.