全文获取类型
收费全文 | 649篇 |
免费 | 86篇 |
国内免费 | 1篇 |
出版年
2021年 | 6篇 |
2019年 | 4篇 |
2018年 | 13篇 |
2017年 | 8篇 |
2016年 | 13篇 |
2015年 | 25篇 |
2014年 | 18篇 |
2013年 | 34篇 |
2012年 | 37篇 |
2011年 | 30篇 |
2010年 | 26篇 |
2009年 | 18篇 |
2008年 | 30篇 |
2007年 | 29篇 |
2006年 | 27篇 |
2005年 | 27篇 |
2004年 | 26篇 |
2003年 | 23篇 |
2002年 | 20篇 |
2001年 | 25篇 |
2000年 | 28篇 |
1999年 | 15篇 |
1998年 | 5篇 |
1997年 | 7篇 |
1996年 | 8篇 |
1995年 | 7篇 |
1994年 | 6篇 |
1992年 | 10篇 |
1991年 | 4篇 |
1990年 | 8篇 |
1989年 | 9篇 |
1988年 | 10篇 |
1987年 | 5篇 |
1986年 | 13篇 |
1985年 | 8篇 |
1984年 | 8篇 |
1983年 | 15篇 |
1982年 | 9篇 |
1981年 | 6篇 |
1979年 | 9篇 |
1978年 | 9篇 |
1976年 | 6篇 |
1975年 | 12篇 |
1974年 | 8篇 |
1973年 | 5篇 |
1972年 | 7篇 |
1971年 | 6篇 |
1970年 | 9篇 |
1968年 | 4篇 |
1967年 | 7篇 |
排序方式: 共有736条查询结果,搜索用时 46 毫秒
721.
722.
723.
B C Clarke P R Shelton G S Mani 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》1988,319(1196):631-640
Computer models of selection acting on a quantitative character show that a combination of frequency-dependent and stabilizing selection can maintain many polymorphisms among the genes that determine the character. The models also show that the random order of mutations can give rise to selectively driven stochastic effects that are sometimes more important than random genetic drift. They suggest simple explanations for patterns of divergence between populations and species, and for apparent discrepancies between the rates of morphological and molecular evolution. They point towards a selective theory of 'molecular clocks'. 相似文献
724.
Adam Takos Daniela Lai Lisbeth Mikkelsen Maher Abou Hachem Dale Shelton Mohammed Saddik Motawia Carl Erik Olsen Trevor L. Wang Cathie Martin Fred Rook 《The Plant cell》2010,22(5):1605-1619
Cyanogenesis, the release of hydrogen cyanide from damaged plant tissues, involves the enzymatic degradation of amino acid–derived cyanogenic glucosides (α-hydroxynitrile glucosides) by specific β-glucosidases. Release of cyanide functions as a defense mechanism against generalist herbivores. We developed a high-throughput screening method and used it to identify cyanogenesis deficient (cyd) mutants in the model legume Lotus japonicus. Mutants in both biosynthesis and catabolism of cyanogenic glucosides were isolated and classified following metabolic profiling of cyanogenic glucoside content. L. japonicus produces two cyanogenic glucosides: linamarin (derived from Val) and lotaustralin (derived from Ile). Their biosynthesis may involve the same set of enzymes for both amino acid precursors. However, in one class of mutants, accumulation of lotaustralin and linamarin was uncoupled. Catabolic mutants could be placed in two complementation groups, one of which, cyd2, encoded the β-glucosidase BGD2. Despite the identification of nine independent cyd2 alleles, no mutants involving the gene encoding a closely related β-glucosidase, BGD4, were identified. This indicated that BGD4 plays no role in cyanogenesis in L. japonicus in vivo. Biochemical analysis confirmed that BGD4 cannot hydrolyze linamarin or lotaustralin and in L. japonicus is specific for breakdown of related hydroxynitrile glucosides, such as rhodiocyanoside A. By contrast, BGD2 can hydrolyze both cyanogenic glucosides and rhodiocyanosides. Our genetic analysis demonstrated specificity in the catabolic pathways for hydroxynitrile glucosides and implied specificity in their biosynthetic pathways as well. In addition, it has provided important tools for elucidating and potentially modifying cyanogenesis pathways in plants. 相似文献
725.
726.
727.
728.
Carina Conceicao Nazia Thakur Stacey Human James T. Kelly Leanne Logan Dagmara Bialy Sushant Bhat Phoebe Stevenson-Leggett Adrian K. Zagrajek Philippa Hollinghurst Michal Varga Christina Tsirigoti Matthew Tully Chris Chiu Katy Moffat Adrian Paul Silesian John A. Hammond Helena J. Maier Erica Bickerton Holly Shelton Isabelle Dietrich Stephen C. Graham Dalan Bailey 《PLoS biology》2020,18(12)
SARS Coronavirus 2 (SARS-CoV-2) emerged in late 2019, leading to the Coronavirus Disease 2019 (COVID-19) pandemic that continues to cause significant global mortality in human populations. Given its sequence similarity to SARS-CoV, as well as related coronaviruses circulating in bats, SARS-CoV-2 is thought to have originated in Chiroptera species in China. However, whether the virus spread directly to humans or through an intermediate host is currently unclear, as is the potential for this virus to infect companion animals, livestock, and wildlife that could act as viral reservoirs. Using a combination of surrogate entry assays and live virus, we demonstrate that, in addition to human angiotensin-converting enzyme 2 (ACE2), the Spike glycoprotein of SARS-CoV-2 has a broad host tropism for mammalian ACE2 receptors, despite divergence in the amino acids at the Spike receptor binding site on these proteins. Of the 22 different hosts we investigated, ACE2 proteins from dog, cat, and cattle were the most permissive to SARS-CoV-2, while bat and bird ACE2 proteins were the least efficiently used receptors. The absence of a significant tropism for any of the 3 genetically distinct bat ACE2 proteins we examined indicates that SARS-CoV-2 receptor usage likely shifted during zoonotic transmission from bats into people, possibly in an intermediate reservoir. Comparison of SARS-CoV-2 receptor usage to the related coronaviruses SARS-CoV and RaTG13 identified distinct tropisms, with the 2 human viruses being more closely aligned. Finally, using bioinformatics, structural data, and targeted mutagenesis, we identified amino acid residues within the Spike–ACE2 interface, which may have played a pivotal role in the emergence of SARS-CoV-2 in humans. The apparently broad tropism of SARS-CoV-2 at the point of viral entry confirms the potential risk of infection to a wide range of companion animals, livestock, and wildlife.A study using a combination of surrogate entry assays and live virus suggests that SARS-CoV-2 may have a broad host-range, revealing that the virus''s spike protein can use a broad range of host ACE2 receptors to enter cells and that the sequence of this protein might have changed during the zoonotic jump into humans. 相似文献
729.
Deborah E. Shelton William W. Driscoll 《Biological reviews of the Cambridge Philosophical Society》2013,88(4):844-861
Biology needs a concept of individuality in order to distinguish organisms from parts of organisms and from groups of organisms, to count individuals and compare traits across taxa, and to distinguish growth from reproduction. Most of the proposed criteria for individuality were designed for ‘unitary’ or ‘paradigm’ organisms: contiguous, functionally and physiologically integrated, obligately sexually reproducing multicellular organisms with a germ line sequestered early in development. However, the vast majority of the diversity of life on Earth does not conform to all of these criteria. We consider the issue of individuality in the ‘minor’ multicellular taxa, which collectively span a large portion of the eukaryotic tree of life, reviewing their general features and focusing on a model species for each group. When the criteria designed for unitary organisms are applied to other groups, they often give conflicting answers or no answer at all to the question of whether or not a given unit is an individual. Complex life cycles, intimate bacterial symbioses, aggregative development, and strange genetic features complicate the picture. The great age of some of the groups considered shows that ‘intermediate’ forms, those with some but not all of the traits traditionally associated with individuality, cannot reasonably be considered ephemeral or assumed transitional. We discuss a handful of recent attempts to reconcile the many proposed criteria for individuality and to provide criteria that can be applied across all the domains of life. Finally, we argue that individuality should be defined without reference to any particular taxon and that understanding the emergence of new kinds of individuals requires recognizing individuality as a matter of degree. 相似文献
730.
Bacillus subtilis glutamine synthetase. Purification and physical characterization 总被引:18,自引:0,他引:18
T F Deuel A Ginsburg J Yeh E Shelton E R Stadtman 《The Journal of biological chemistry》1970,245(20):5195-5205