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91.
James A. Higgins Mark C. Jenkins Daniel R. Shelton Ron Fayer Jeffrey S. Karns 《Applied microbiology》2001,67(11):5321-5324
The Xtra Amp tube, Isocode paper, Instagene matrix, and PrepMan matrix methods were evaluated for their ability to rapidly extract PCR-quality DNAs from Escherichia coli O157:H7 and Cryptosporidium parvum. All methods provided satisfactory DNA from E. coli, and the Xtra Amp and Instagene reagents provided satisfactory DNA from C. parvum. 相似文献
92.
J A Higgins M C Jenkins D R Shelton R Fayer J S Karns 《Applied and environmental microbiology》2001,67(11):5321-5324
The Xtra Amp tube, Isocode paper, Instagene matrix, and PrepMan matrix methods were evaluated for their ability to rapidly extract PCR-quality DNAs from Escherichia coli O157:H7 and Cryptosporidium parvum. All methods provided satisfactory DNA from E. coli, and the Xtra Amp and Instagene reagents provided satisfactory DNA from C. parvum. 相似文献
93.
G A Shelton 《The Journal of experimental biology》1975,62(2):421-432
1. The SS 1 fatigues in response to repetitive electrical stimulation. This fatigue is manifested by an increased conduction delay and a decreased SS 1 pulse amplitude. 2. Continued repetitive stimulation leads to the failure of the system. Recovery may take many seconds. Narrow strips of column fail more rapidly than wide strips. 3. The increased conduction delay is explained in terms of a decrease in the population of spiking cells. 4. A computer model is described and analysed. It suggests that conduction between electrically coupled ectoderm cells could be the basis for the SS1. The SS 1 may have properties not so far experimentally demonstrated; for example, under certain conditions it could behave as a local system. 相似文献
94.
95.
G A Shelton 《The Journal of experimental biology》1975,62(3):571-578
1. The octocorals Alcyonium digitatum, Pennatula phosphorea and Virgularia mirabilis each have a through-conducting nerve net. The nerve net demonstrated electrophysiologically may well be the same as that previously shown by the use of histological techniques. 2. It exhibits both facilitation and defacilitation in the rate of conduction of pulses. 3. The distance of spread of nerve net activity is not limited by the number of stimuli applied. 4. The nerve net controls fast muscle contractions; the frequency of pulses is important in determining which muscles contract and in which sequence. 5. The nerve net is 'spontaneously' active. 6. A previously undescirbed slow system has been identified in Pennatula. It has many of the properties of slow systems in sea anemones and may well be ectodermal. It is suggested that multiple conduction systems are of common occurrence in the Anthozoa. 相似文献
96.
C. Shelton A. G. Macdonald A. Pequeux I. Gilchrist 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1985,155(5):629-633
Summary This paper reports measurements of the plasma osmolarities, the main inorganic ions and the concentrations of plasma proteins
in nine species of deep sea fish from depths of 900–4,000 m.
Chloride and magnesium values are high compared to those of shallow water fish. These ions may be contaminants from sea water
either contributing to, or resulting from, the animals' moribund condition. They may also contribute to the failure of isolated
erythrocytes to show ionic regulation even after being restored to their normal high pressure. 相似文献
97.
An apparatus for monitoring efflux rates of specific substances from cellular preparations is described. Tissue samples (homogenates, subcellular fractions, small tissue slices, cell suspensions etc.) are placed on a filter, perfused with several different media sequentially and aliquots of the perfusate collected at intervals of 5 sec. Under maximum perfusion rates, the changeover in perfusion media is completed in less than 1 sec, produces no detectable disturbance of the sample and allows only minimal mixing of the different media. The apparatus has been used successfully to study stimulus secretion coupling during release of the neurotransmitter [14C]γ-aminobutyric acid from synaptosomes. 相似文献
98.
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Outcomes of endoscopic ultrasound‐guided pancreatic FNAC diagnosis for solid and cystic lesions at Manchester Royal Infirmary based upon the Papanicolaou Society of Cytopathology pancreaticobiliary terminology classification scheme
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P. K. Wright D. A. Shelton M. R. Holbrook S. A. Thiryayi N. Narine D. Slater D. N. Rana 《Cytopathology》2018,29(1):71-79