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81.
The male annihilation technique (MAT) and sterile insect technique (SIT) are often used to control pestiferous tephritid fruit flies (Diptera: Tephritidae). MAT involves the deployment of traps containing a male attractant and insecticide with the goal of drastically reducing male abundance and ultimately eliminating the entire population. SIT, which involves the mass production, sterilization, and release of the target species, may also be implemented to achieve final extirpation. Generally, simultaneous implementation of MAT and SIT is counterproductive, because the presence of large numbers of male-specific traps in the environment (MAT) would greatly reduce the number of sterile males available for copulating with wild females (SIT). However, studies on the Queensland fruit fly, Bactrocera tryoni (Froggatt), indicate that concurrent use of MAT and SIT may be feasible. Sexually mature males of B. tryoni are attracted to the raspberry ketone and its synthetic analogue cue-lure. Males of B. tryoni fed raspberry-ketone-supplemented diet when newly emerged showed lower attraction to cue-lure baited traps than control males. In addition, newly emerged males provided this diet displayed accelerated sexual maturation, which would allow the early release of sterile males and reduce pre-release holding costs. Here, we examined whether the addition of raspberry ketone to the adult diet of male melon flies, Zeugodacus cucurbitae (Coquillett), produced effects similar to those observed for B. tryoni. Despite using similar methods, no significant effect of raspberry ketone-supplemented diet on time to sexual maturity, survival, mating competitiveness, or attraction to cue-lure baited traps in mass-reared Z. cucurbitae males. 相似文献
82.
Kelsey M. McCoy Emily S. Tubman Allison Claas Damien Tank Shelly Applen Clancy Eileen T. O’Toole Judith Berman David J. Odde 《Molecular biology of the cell》2015,26(22):3999-4014
A characteristic feature of mitotic spindles is the congression of chromosomes near the spindle equator, a process mediated by dynamic kinetochore microtubules. A major challenge is to understand how precise, submicrometer-scale control of kinetochore microtubule dynamics is achieved in the smallest mitotic spindles, where the noisiness of microtubule assembly/disassembly will potentially act to overwhelm the spatial information that controls microtubule plus end–tip positioning to mediate congression. To better understand this fundamental limit, we conducted an integrated live fluorescence, electron microscopy, and modeling analysis of the polymorphic fungal pathogen Candida albicans, which contains one of the smallest known mitotic spindles (<1 μm). Previously, ScCin8p (kinesin-5 in Saccharomyces cerevisiae) was shown to mediate chromosome congression by promoting catastrophe of long kinetochore microtubules (kMTs). Using C. albicans yeast and hyphal kinesin-5 (Kip1p) heterozygotes (KIP1/kip1∆), we found that mutant spindles have longer kMTs than wild-type spindles, consistent with a less-organized spindle. By contrast, kinesin-8 heterozygous mutant (KIP3/kip3∆) spindles exhibited the same spindle organization as wild type. Of interest, spindle organization in the yeast and hyphal states was indistinguishable, even though yeast and hyphal cell lengths differ by two- to fivefold, demonstrating that spindle length regulation and chromosome congression are intrinsic to the spindle and largely independent of cell size. Together these results are consistent with a kinesin-5–mediated, length-dependent depolymerase activity that organizes chromosomes at the spindle equator in C. albicans to overcome fundamental noisiness in microtubule self-assembly. More generally, we define a dimensionless number that sets a fundamental physical limit for maintaining congression in small spindles in the face of assembly noise and find that C. albicans operates very close to this limit, which may explain why it has the smallest known mitotic spindle that still manifests the classic congression architecture. 相似文献
83.
We perform rhythmic and discrete arm movements on a daily basis, yet the motor control literature is not conclusive regarding the mechanisms controlling these movements; does a single mechanism generate both movement types, or are they controlled by separate mechanisms? A recent study reported partial asymmetric transfer of learning from discrete movements to rhythmic movements. Other studies have shown transfer of learning between large-amplitude to small-amplitude movements. The goal of this study is to explore which aspect is important for learning to be transferred from one type of movement to another: rhythmicity, amplitude or both. We propose two hypotheses: (1) Rhythmic and discrete movements are generated by different mechanisms; therefore we expect to see a partial or no transfer of learning between the two types of movements; (2) Within each movement type (rhythmic/discrete), there will be asymmetric transition of learning from larger movements to smaller ones. We used a learning-transfer paradigm, in which 70 participants performed flexion/extension movements with their forearm, and switched between types of movement, which differed in amplitude and/or rhythmicity. We found partial transfer of learning between discrete and rhythmic movements, and an asymmetric transfer of learning from larger movements to smaller movements (within the same type of movement). Our findings suggest that there are two different mechanisms underlying the generation of rhythmic and discrete arm movements, and that practicing on larger movements helps perform smaller movements; the latter finding might have implications for rehabilitation. 相似文献
84.
Quantifying the importance of geographic replication and representativeness when estimating demographic rates,using a coastal species as a case study
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Christopher R. Field Katharine J. Ruskin Bri Benvenuti Alyssa C. Borowske Jonathan B. Cohen Laura Garey Thomas P. Hodgman Rebecca A. Longenecker Erin King Alison R. Kocek Adrienne I. Kovach Kathleen M. O'Brien Brian J. Olsen Nancy Pau Samuel G. Roberts Emma Shelly W. Gregory Shriver Jennifer Walsh Chris S. Elphick 《Ecography》2018,41(6):971-981
Demographic rates are rarely estimated over an entire species range, limiting empirical tests of ecological patterns and theories, and raising questions about the representativeness of studies that use data from a small part of a range. The uncertainty that results from using demographic rates from just a few sites is especially pervasive in population projections, which are critical for a wide range of questions in ecology and conservation. We developed a simple simulation to quantify how this lack of geographic representativeness can affect inferences about the global mean and variance of growth rates, which has implications for the robust design of a wide range of population studies. Using a coastal songbird, saltmarsh sparrow Ammodramus caudacutus, as a case study, we first estimated survival, fecundity, and population growth rates at 21 sites distributed across much of their breeding range. We then subsampled this large, representative dataset according to five sampling scenarios in order to simulate a variety of geographic biases in study design. We found spatial variation in demographic rates, but no large systematic patterns. Estimating the global mean and variance of growth rates using subsets of the data suggested that at least 10–15 sites were required for reasonably unbiased estimates, highlighting how relying on demographic data from just a few sites can lead to biased results when extrapolating across a species range. Sampling at the full 21 sites, however, offered diminishing returns, raising the possibility that for some species accepting some geographical bias in sampling can still allow for robust range‐wide inferences. The subsampling approach presented here, while conceptually simple, could be used with both new and existing data to encourage efficiency in the design of long‐term or large‐scale ecological studies. 相似文献
85.
Shelly Volsche 《Anthrozo?s》2018,31(3):367-377
The American Pet Products Association reports a decade-long increase of pet spending, estimated to reach over $69 billion in 2017. In some cases, these owners apply traditional parenting practices while nurturing their pets. Relatedly, a growing number of pet owners identify themselves as “pet parents.” This study is the first known work to investigate how identity plays a role in the growing population of pet parents. While previous research into the pet parenting phenomenon has sought to quantify the demographics, spending, and frequency of this population, less work seeks to understand what pet parenting looks like in practice. Sampling a population of childfree pet parents, the current study utilized semi-structured interviews and identified two common themes in their daily practice of the identity: 1) Despite the use of parent–child terms and strategies, childfree pet parents are acutely aware of the differences between raising children and raising pets, and 2) pet parents ascribe agency to their pet, identifying individual pet wants and needs to fulfill. This may further inform future research on human–animal relationships by establishing a human identity that connects directly to pet practices in the United States, asking us to consider the lived experiences of this growing population and their pets including spending, lifestyle, and attachment concerns. 相似文献
86.
Many countries operate trapping programs to detect invasions of pestiferous fruit fly species (Diptera: Tephritidae). Surveillance relies heavily on traps baited with male lures, which, while highly attractive, have limited effectiveness, because (i) they are sex-specific and (ii) males of some species do not respond to the lures currently in use. For these reasons, detection programs also include food-baited traps that are neither sex- nor species-specific. Compared to male lure-baited traps, however, few studies have measured the attractiveness of food-based traps. The present study describes a mark-release-recapture study conducted in a fruit orchard in Hawaii that measured the attractiveness of a liquid protein hydrolysate-based (torula yeast/borax slurry) trap to adults of the melon fly Zeugodacus cucurbitae (Coquillett). Multiple release points were used at varying distances from a single, central trap to generate estimates of distance-dependent capture probabilities. The potential influences of sex and pre-release diet on capture probability were also examined. Flies were released at 14 d of age and were maintained on one of four dietary regimes that offered a protein hydrolysate-rich diet for varying intervals (i.e., 0, 3, 7, or 14?d, respectively). Recapture rates were similar between the sexes and over both sexes and all diets averaged 3.6%, 3.2%, and 0.6% for release distances of 10, 25, and 50?m, respectively. Pre-release diet had a significant effect on recapture probability for releases at 10 and 25?m: flies fed sugar only or protein hydrolysate-rich diet for only 3?d were captured more frequently than flies that had longer access to yeast extract prior to release. 相似文献
87.
88.
Insung Na Shelly DeForte Bosko M. Stojanovski 《Journal of biomolecular structure & dynamics》2018,36(1):152-165
Heme biosynthesis, a complex, multistage, and tightly controlled process, starts with 5-aminolevulinate (ALA) production, which, in metazoa and certain bacteria, is a reaction catalyzed by 5-aminolevulinate synthase (ALAS), a pyridoxal 5′-phosphate (PLP)-dependent enzyme. Functional aberrations in ALAS are associated with several human diseases. ALAS can adopt open and closed conformations, with segmental rearrangements of a C-terminal, 16-amino acid loop and an α-helix regulating accessibility to the ALAS active site. Of the murine erythroid ALAS (mALAS2) forms previously engineered to assess the role of the flexible C-terminal loop versus mALAS2 function one stood out due to its impressive gain in catalytic power. To elucidate how the simultaneously introduced seven mutations of this activity-enhanced variant affected structural and dynamic properties of mALAS2, we conducted extensive molecular dynamics simulation analysis of the dimeric forms of wild-type mALAS2, hepta-variant and Rhodobacter capsulatus ALAS (aka R. capsulatus HemA). This analysis revealed that the seven simultaneous mutations in the C-terminal loop, which extends over the active site of the enzyme, caused the bacterial and murine proteins to adopt different conformations. Specifically, a new β-strand in the mutated ‘loop’ led to interaction with two preexisting β-strands and formation of an anti-parallel three-stranded β-sheet, which likely endowed the murine hepta-variant a more ‘stable’ open conformation than that of wild-type mALAS2, consistent with a kinetic mechanism involving a faster closed-to-open conformation transition and product release for the mutated than wild-type enzyme. Further, the dynamic behavior of the mALAS2 protomers was strikingly different in the two dimeric forms. 相似文献
89.
Chl fluorescence was used to measure the photosynthetic capacity of the green alga Dunaliella tertiolecta in order to investigate interactions between susceptibility to acute UV‐B radiation (UVBR, 280–320 nm) exposure and decreased nitrogen availability. Under UVBR exposure the decline in the fluorescence parameters Fv/Fm (the maximum effective quantum yield ΦPSIIe‐max) and Fv′/Fm′ (the operational quantum yield of PSII, ΦPSIIe) were enhanced with higher UVBR fluxes, with the data well described by the Kok model, inferring that a dynamic balance existed between damage and repair with the repair proportional to the pool size of inactivated targets. When UVBR exposure was coupled with nitrogen limitation, the inhibition of photosynthesis was intensified. Under the more severely N‐limited conditions, the damage rate increased. Unexpectedly, repair rates were also stimulated under N‐limited conditions, although this was insufficient to counteract the increase in damage, so the overall effect of N limitation was an enhancement of UVBR‐induced inhibition of photosynthesis. 相似文献
90.
Annual productivity and excretion of organic compounds by epibenthic algae in a coastal Texas marsh were investigated using 14C techniques. Over the course of two years, productivity and extracellular release were measured bimonthly to assess the environmental factors affecting seasonal patterns. Annual productivity was estimated to be 71 gc·m?2, approximately 10% of the estimated net aerial productivity of the grass canopy in this area. The percentage of photoassimilated carbon released was fairly constant, and the rate of excretion appeared to be primarily dependent upon the rate of carbon fixation. Greater rates of release were observed with high salinity, low soil moisture conditions. It was estimated that up to 10% of the DOC in adjacent tidal creek water could be produced by this community and could represent an important source of nutrients for consumers in the system. 相似文献