Conformational analysis of 5-substituted uracil

The high-resolution IR-spectra of 5-nitrouracil and 5-bromouracil isolated in Ar matrices at 11 K were obtained for the first time. The conformational structure of uracil 5-substituents--thymine, 5-bromouracil, 5-nitrouracil--is calculated by the molecular mechanics and quantum--chemical MINDO/3 methods. The possibility of thymine transition to nonplanar conformations is observed.     

Isolation of germ-cell precursors from human ovary tissue

This article describes the isolation of germ-cell precursors from human ovary tissues collected during various gynecological operations. Two cell cultures were established from tunica albuginea tissue. These cells were assayed by immunocytochemical and histological staining and PCR. The study confirms the presence of precursor cells in ovaries of reproductive age women.     

Infrared spectra of associated and hydrated pyrimidine bases of nucleic acids in argon matrix

The spectral characteristics of interacting pyrimidine bases of nucleic acids--cytosine, 1-methylcytosine, uracil in autoassociates and hydrates--are investigated using the high-resolution infrared spectroscopy in argon matrices. Shifts of vibration frequencies in simple complexes are stated to be from 10 to 30 cm-1 in the 4000-400 cm-1 region observed. The isolated state-solid phase transition is followed by the absorption maximum shift, the latter being some hundreds of cm-1 in the 4000-2000 cm-1 region and some tens of cm-1 in the 1800-400 cm-1 region.