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701.
Characterization of Two New Genes, amoR and amoD, in the amo Operon of the Marine Ammonia Oxidizer Nitrosococcus oceani ATCC 19707
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Molecular analysis of the amo gene cluster in Nitrosococcus oceani revealed that it consists of five genes, instead of the three known genes, amoCAB. The two additional genes, orf1 and orf5, were introduced as amoR and amoD, respectively. Putative functions of the AmoR and AmoD proteins are discussed. 相似文献
702.
Tiina Myyryläinen Sheikh M Talha Sathyamangalam Swaminathan Raija Vainionpää Tero Soukka Navin Khanna Kim Pettersson 《Journal of nanobiotechnology》2010,8(1):27
A highly specific and novel dual-label time-resolved immunofluorometric assay was developed exploiting the unique emission
wavelengths of the intrinsically fluorescent terbium (Tb3+) and europium (Eu3+) tracers for the simultaneous detection of human immunodeficiency virus 1 (HIV-1) and hepatitis B virus (HBV) infections,
respectively. HIV-1 infection was detected using a double antigen sandwich format wherein anti-HIV-1 antibodies were captured
using an in vivo biotinylated version of a chimeric HIV-1 antigen and revealed using the same antigen labeled with Tb3+ chelate. Hepatitis B surface antigen (HBsAg), which served as the marker of HBV infection, was detected in a double antibody
sandwich using two monoclonal antibodies (mAbs), one chemically biotinylated to capture, and the other labeled with Eu3+ nanoparticles, to reveal. The performance of the assay was evaluated using a collection (n = 60) of in-house and commercially
available human sera panels. This evaluation showed the dual-label assay to possess high degrees of specificity and sensitivity,
comparable to those of commercially available, single analyte-specific kits for the detection of HBsAg antigen and anti-HIV
antibodies. This work demonstrates the feasibility of developing a potentially time- and resource-saving multiplex assay for
screening serum samples for multiple infections in a blood bank setting. 相似文献
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706.
Seed germination and salinity tolerance in plant species growing on saline wastelands 总被引:1,自引:0,他引:1
Seven plant species including three chenopods:Suaeda fruticosa, Kochia indica, Atriplex crassifolia and four grasses:Sporobolus arabicus, Cynodon dactylon, Polypogon monspeliensis, Desmostachya bipinnata, varied greatly in their seed germination and growth responses to soil moisture or salinity. The germination percentage of
each species was significantly lower at soil moisture level of 25 % of water holding capacity than at the levels ranging from
50 to 125 %. Increase in salinity resulted in gradual decrease in seed germination of each species. Growth responses of species
to salinity varied widely from significant decrease with slight salinity to stimulation up to salinity levels of 20 dS m-2. Higher K+Na+ratios in plant shoots of all species compared to that in the root medium indicated selective K+uptake. Higher tolerance in chenopod species seems to be attendant on their ability for internal ion regulation.
We are thankful to Mr. Noor Ahmad for his assistance in experimental work. 相似文献
707.
A. Raza M. A. Miller C. Mazewski Y. Sheikh B. Lampkin R. Sawaya† K. Crone† T. Berger† J. Reising‡ J. Gray§ S. Khan H. Preisler 《Cell proliferation》1991,24(2):113-126
In studies using bromodeoxyuridine (BrdUrd) and/or iododeoxyuridine (IdUrd) to label S phase cells in cancer patients, several unique observations were made regarding DNA replication sites and the organization of newly synthesized DNA in post-mitotic cells. While the majority of tumour specimens removed at the end of infusions demonstrated concentration of replication sites around the nuclear membrane, biopsies obtained in leukaemic patients 1 week later demonstrated several distinct patterns of labelling. For example, one, two or all lobes of granulocytes were labelled. Scavenger macrophages bearing labelled leukaemic cells in their cytoplasm were also seen. Sequential IdUrd/BrdUrd labelling of solid tumours showed various patterns of nuclear/nucleolar/membrane labelling, allowing more precise localization of early versus late replication sites. 相似文献
708.