全文获取类型
收费全文 | 170篇 |
免费 | 4篇 |
专业分类
174篇 |
出版年
2023年 | 4篇 |
2022年 | 6篇 |
2021年 | 9篇 |
2020年 | 2篇 |
2019年 | 8篇 |
2018年 | 3篇 |
2017年 | 6篇 |
2016年 | 6篇 |
2015年 | 5篇 |
2014年 | 10篇 |
2013年 | 15篇 |
2012年 | 18篇 |
2011年 | 8篇 |
2010年 | 8篇 |
2009年 | 5篇 |
2008年 | 7篇 |
2007年 | 7篇 |
2006年 | 5篇 |
2005年 | 7篇 |
2004年 | 5篇 |
2003年 | 4篇 |
2002年 | 4篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1995年 | 4篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1980年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1968年 | 1篇 |
1966年 | 2篇 |
排序方式: 共有174条查询结果,搜索用时 0 毫秒
101.
Varun Garg Harmanpreet Singh Amit Bhatia Kaisar Raza Sachin Kumar Singh Bhupinder Singh Sarwar Beg 《AAPS PharmSciTech》2017,18(1):58-71
Piroxicam is used in the treatment of rheumatoid arthritis, osteoarthritis, and other inflammatory diseases. Upon oral administration, it is reported to cause ulcerative colitis, gastrointestinal irritation, edema and peptic ulcer. Hence, an alternative delivery system has been designed in the form of transethosome. The present study describes the preparation, optimization, characterization, and ex vivo study of piroxicam-loaded transethosomal gel using the central composite design. On the basis of the prescreening study, the concentration of lipids and ethanol was kept in the range of 2–4% w/v and 0–40% v/v, respectively. Formulation was optimized by measuring drug retention in the skin, drug permeation, entrapment efficiency, and vesicle size. Optimized formulation was incorporated in hydrogel and compared with other analogous vesicular (liposomes, ethosomes, and transfersomes) gels for the aforementioned responses. Among the various lipids used, soya phosphatidylcholine (SPL 70) and ethanol in various percentages were found to affect drug retention in the skin, drug permeation, vesicle size, and entrapment efficiency. The optimized batch of transethosome has shown 392.730 μg cm?2 drug retention in the skin, 44.312 μg cm?2 h?1 drug permeation, 68.434% entrapment efficiency, and 655.369 nm vesicle size, respectively. It was observed that the developed transethosomes were found superior in all the responses as compared to other vesicular formulations with improved stability and highest elasticity. Similar observations were noted with its gel formulation. 相似文献
102.
103.
Bogdan JA Yuan W Long-Rowe KO Sarwar J Brucker EA Blake MS 《Applied and environmental microbiology》2003,69(10):6272-6279
The introduction of acellular pertussis vaccines has greatly enhanced the safety profile of vaccines to prevent whooping cough. Pertussis toxin (Ptx) is one component produced by Bordetella pertussis that is contained in all of these vaccines, either in combination with other known pertussis virulence factors or as the sole pertussis component, combined with tetanus and diphtheria toxoids. A hydrogen peroxide toxoid of Ptx has been shown to be efficacious in preventing pertussis infections in a mass vaccination trial and is presently licensed in the United States and Europe (B. Trollfors, J. Taranger, T. Lagergard, L. Lind, V. Sundh, G. Zackrisson, C. U. Lowe, W. Blackwelder, and J. B. Robbins, N. Engl. J. Med. 333:1045-1050, 1995). The industrial production of Ptx can be performed through the cultivation of B. pertussis in well-defined growth media, in which the components can be well characterized and their origins can be documented. Once the bacteria are removed from the culture, Ptx can be isolated from the supernatant and purified by using the technique described by Sekura et al. (R. D. Sekura, F. Fish, C. R. Manclark, B. Meade, and Y. L. Zhang, J. Biol. Chem. 258:14647-14651, 1983). The only drawback of this procedure, which combines two affinity chromatography steps, one with Blue Sepharose and a second with matrix-bound bovine fetuin (BF), is the source and purity of the BF. Concern about vaccine preparations that may possibly risk contamination by material associated with bovine spongioform encephalopathy has continued to increase. We thus sought a replacement for the BF affinity chromatography and, more specifically, for the glycosidic moiety on BF. We describe here the identification of a seven-amino-acid peptide that mimics the glycosidic moiety on BF to which Ptx binds. Furthermore, we have constructed an affinity column containing this peptide that can be used to replace BF in Ptx purification. Finally, we used the X-ray crystallographic structure of Ptx bound to the oligosaccharide moiety of BF as a scaffold and replaced the oligosaccharide with the peptide. 相似文献
104.
Alrefai WA Annaba F Sarwar Z Dwivedi A Saksena S Singla A Dudeja PK Gill RK 《American journal of physiology. Gastrointestinal and liver physiology》2007,292(1):G369-G376
Niemann-Pick C1-like 1 (NPC1L1) is an essential intestinal component of cholesterol absorption. However, little is known about the molecular regulation of intestinal NPC1L1 expression and promoter activity. We demonstrated that human NPC1L1 mRNA expression was significantly decreased by 25-hydroxycholesterol but increased in response to cellular cholesterol depletion achieved by incubation with Mevinolin (an inhibitor of 3-hydroxy-3-methylglutaryl-CoA reductase) in human intestinal Caco-2 cells. We also showed that a -1741/+56 fragment of the NPC1L1 gene demonstrated high promoter activity in Caco-2 cells that was reduced by 25-hydroxycholesterol and stimulated by cholesterol depletion. Interestingly, we showed that the NPC1L1 promoter is remarkably transactivated by the overexpression of sterol regulatory element (SRE) binding protein (SREBP)-2, suggesting its involvement in the sterol-induced alteration in NPC1L1 promoter activity. Finally, we identified two putative SREs in the human NPC1L1 promoter and established their essential roles in mediating the effects of cholesterol on promoter activity. Our study demonstrated the modulation of human NPC1L1 expression and promoter activity by cholesterol in a SREBP-2-dependent mechanism. 相似文献
105.
A. K. M. Golam Sarwar Md. Sabibul Haque Md. Ekramul Haque Md. Amir Hossain Md. Golam Azam Md. Nesar Uddin Eldessoky S. Dessoky Mahmoud A. Basry Md. Alamgir Hossain 《Phyton》2022,91(8):1721-1743
Linseed is a multipurpose crop and the crop needs further improvement to increase production and yield due to
its high value and demand. This study aimed to assess the extent and pattern of genetic variability of forty linseed
genotypes based on diverse agro–morphological and yield attributes. The field experiment was conducted following a Randomized Complete Block Design with three replications. Linseed germplasm showed a wide range of
phenotypic expression, genetic variability and heritability for 30 studied traits. A low to high phenotypic coeffi-
cient of variation (PCV) and genotypic coefficient of variation (GCV) were observed. The lowest genotypic (σ2
g)
and phenotypic variances (σ2
p) were found in capsule diameter (CD), length of calyx (LC), capsule length (CL),
seed length (SL), and seed breadth (SB). High broad-sense heritability (h2b) with high genetic advance as a percentage of mean (GAM) were observed in days to germination started (DGS), days to 80% emergence (DE), plant
height at 28 and 40 DAS, number of flowers (NFPP), filled capsules (NFCPP) and yield per plant (YPP) indicating
additive gene action exists for these characters. Hierarchical cluster analysis separated 40 genotypes into five clusters, where Clusters I to V assembled with 13, 4, 4, 5 and 14 genotypes, respectively. Considering yield and yield
attributes, Cluster-IV (G3, G4, G6, G10 and G31) genotypes showed promising while, Cluster-II (G2, G16, G35,
G36) and Cluster-III (G1, G33, G39 and G40) genotypes were dominant on plant morphological traits. Based on
principal component analysis (PCA), few characters such as YPP, NFPP, NFCPP, days to first flowering and capsule formation, early emergence, days to branch initiation and plant heights at different growth stages revealed
important and effective traits for consideration in the selection of linseed breeding programs. 相似文献
106.
Abdul Latif Saeed Ahmad Malik Shafqat Saeed Syed Muhammad Zaka Zahid Mahmood Sarwar Muqarrab Ali Muhammad Farooq Azhar Muhammad Javaid Muhammad Ishtiaq Unsar Naeem-Ullah Mamoona Naoreen Khalid Ali Khan Hamed A. Ghramh Muhammad Ahmed Shahzad 《Saudi Journal of Biological Sciences》2019,26(7):1548-1552
Indian siris, Albizia lebbeck (L.) Benth. (Fabaceae: Mimosoideae) has significant importance to human beings for its multipurpose use. Insects play a crucial role in the pollination biology of flowering plants. In the current study, we studied the pollination biology of A. lebbeck with special reference to insect floral visitors. The effectiveness of floral visitors was investigated in term of visitation frequency, visitation rate and pollen load during 2012 and 2013. In the second experiment, effect of pollinators on yield of A. lebbeck was studied in open and cage pollination experiments. Floral visitor fauna of A. lebbeck included eight-bees, two wasps, two flies, and two butterflies species. Among them, Apis dorsata, Apis florea, Amegilla cingulata, and Nomia oxybeloides had maximum abundance ranging from 349–492, 339–428, 291–342 and 235–255 numbers of individuals, respectively during two flowering seasons. A. dorsata had the highest visitation frequency (6.44 ± 0.49–8.78 ± 0.48 visits/flower/5min) followed by Amegilla cingulata (6.03 ± 0.43–7.99 ± 0.33 visits/flower/5min) and A. florea (3.61 ± 0.31–4.44 ± 0.18 visits/flower/5min). A. dorsata, N. oxybeloides, and Amegilla cingulata had the highest visitation rates (18.904 ± 1.53–11.43 ± 1.17 flower visited/min) and pollen load (15333 ± 336.22–19243 ± 648.45 pollen grains). The open pollinated flowers had significantly higher capsule weight (4.97 ± 0.21 g), seed weight (1.04 ± 0.05 g), seed numbers per pod (9.80 ± 0.34) and seed germination percentage (84.0 ± 1.78%) as compared to caged flowers. The results suggested bees especially A. dorsata, N. oxybeloides and Amegilla cingulata could be effective pollinators of A. lebbeck. 相似文献
107.
Recently, the attention of researchers has been drawn toward the synthesis of chitosan derivatives and their nanoparticles with enhanced antimicrobial activities. In this study, chitosan derivatives with different azides and alkyne groups were synthesized using click chemistry, and these were further transformed into nanoparticles by using the ionotropic gelation method. A series of chitosan derivatives was successfully synthesized by regioselective modification of chitosan via an azide-alkyne click reaction. The amino moieties of chitosan were protected during derivatization by pthaloylation and subsequently unblocked at the end to restore their functionality. Nanoparticles of synthesized derivatives were fabricated by ionic gelation to form complexes of polyanionic penta-sodium tripolyphosphate (TPP) and cationic chitosan derivatives. Particle size analysis showed that nanoparticle size ranged from 181.03 ± 12.73 nm to 236.50 ± 14.32 nm and had narrow polydispersity index and positive surface charge. The derivatives and corresponding nanoparticles were evaluated in vitro for antibacterial and antifungal activities against three gram-positive and gram-negative bacteria and three fungal strains, respectively. The minimum inhibitory concentration (MIC) of all derivatives ranged from 31.3 to 250 µg/mL for bacteria and 188 to1500 µg/mL for fungi and was lower than that of native chitosan. The nanoparticles with MIC ranging from 1.56 to 25 µg/mLfor bacteria and 94 to 750 µg/mL for fungi exhibited higher activity than the chitosan derivatives. Chitosan O-(1-methylbenzene) triazolyl carbamate and chitosan O-(1-methyl phenyl sulfide) triazolyl carbamate were the most active against the tested bacterial and fungal strains. The hemolytic assay on erythrocytes and cell viability test on two different cell lines (Chinese hamster lung fibroblast cells V79 and Human hepatic cell line WRL68) demonstrated the safety; suggesting that these derivatives could be used in future medical applications. Chitosan derivatives with triazole functionality, synthesized by Huisgen 1,3-dipolar cycloaddition, and their nanoparticles showed significant enhancement in antibacterial and antifungal activities in comparison to those associated with native, non-altered chitosan. 相似文献
108.
109.
Katherine S Elliott Marc Haber Hinda Daggag George B Busby Rizwan Sarwar Derek Kennet Michael Petraglia Lawrence J Petherbridge Parisa Yavari Frauke U Heard-Bey Bindu Shobi Tariq Ghulam Dalia Haj Alia Al Tikriti Alshafi Mohammad Suma Antony Maitha Alyileili Shatha Alaydaroos Evelyn Lau Mark Butler Arash Yavari Julian C Knight Houman Ashrafian Maha T Barakat 《Molecular biology and evolution》2022,39(3)
The indigenous population of the United Arab Emirates (UAE) has a unique demographic and cultural history. Its tradition of endogamy and consanguinity is expected to produce genetic homogeneity and partitioning of gene pools while population movements and intercontinental trade are likely to have contributed to genetic diversity. Emiratis and neighboring populations of the Middle East have been underrepresented in the population genetics literature with few studies covering the broader genetic history of the Arabian Peninsula. Here, we genotyped 1,198 individuals from the seven Emirates using 1.7 million markers and by employing haplotype-based algorithms and admixture analyses, we reveal the fine-scale genetic structure of the Emirati population. Shared ancestry and gene flow with neighboring populations display their unique geographic position while increased intra- versus inter-Emirati kinship and sharing of uniparental haplogroups, reflect the endogamous and consanguineous cultural traditions of the Emirates and their tribes. 相似文献
110.