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A new method, based on potential measurements to obtain permeabilities and mobilities of ionic species passing through membranes,
under the influence of concentration gradients is proposed. This method is applied to three different lipid membranes in aqueous
KCl solutions. The values of permeabilities and mobilities obtained by this simple method is in good agreement with those
reported in literature 相似文献
234.
Characteristics of fungal species tolerant to high levels of metals in natural environment can be amplified by isolation and
selection of resistant mutants. Step-by-step culturing led to identification of highly stable Co-resistant (CoR) mutants of A. nidulans. Based on two distinct morphological features, Co-resistant mutants were categorized as CoRI and CoRII. The two mutants varied in their growth behavior and colony morphology that were reflected in supplemented as well as unsupplemented
growth media over the generations. As compared to the CoRI, CoRII mutant exhibited sparse mycelia and conidiation but secreted higher amount of melanin. CoR mutants could tolerate up to 2.5mM Co in the medium, however, required a threshold concentration of 0.25mM Co for optimal
growth and germination. Absence of Co in the medium caused a stressful situation for the CoR mutants and led to the secretion of a white extracellular precipitate found to be a glycoprotein. In response to interactions
with Co-ions, CoR mutants produced oxalic acid and bioprecipitated Co as Co-oxalate providing scope for metal reclamation as well as oxalic
acid extraction. The mutants could help to recover the insoluble Co-oxalate salt from aqueous solutions by entrapping it in
their growing mycelial meshwork. 相似文献