Phase transition in natural collagen]

The state of water protons and peptide chains in natural collagen was investigated by the method of nuclear magnetic resonance. The study of orientation and temperature dependence of collagen PMR spectra allows us to mage a conclusion about the considerable disorder of mutual orientation in the polypeptide chains at the temperature above -4 degrees C. Under these conditions it was observed that natural collagen was near to the liquid-crystal. The phasic transition at -4 degrees C was discovered, the latter was followed by an abrupt change of the character of water proton mobility and by the change of orientation of collagen fibres order. The thermal effect of transition was measured, the latter presents 18k/gr of the whole specimen.     

Chemotherapy of Campylobacter infections]

A total of 600 patients with suspected alimentary food poisoning were hospitalized. The ++clinico-laboratory findings showed that 27 (4.5 per cent) of them had Campylobacter infection. The cultures of Campylobacter jejuni and Campylobacter coli were isolated from 24 and 3 patients, respectively. The patients underwent complex pathogenetic treatment with oral rehydration saline solutions, symptomatic agents, enzymatic preparations and diet (the basic therapy) supplemented with biological bacterial preparations in less severe cases (8 patients). Antibacterial drugs such as furazolidone (roxytromycin), gentamicin and levomycetin, as well as fluoroquinolones such as ofloxacin and ciprofloxacin were additionally used in the treatment of both the patients with the generalized infection and those with more severe processes of the disease, pronounced diarrhea with blood traces and persisting isolation of Campylobacter (12 patients). The most favourable results in the treatment of more severe patients with Campylobacter infection were obtained with the fluoroquinolones used after inadequately efficient therapy with furazolidone or antibiotics especially in the cases with repeated isolation of campylobacteria.     

Contritution of correction to genetic recombination in T4 phage measured by the effect of map contraction

Marker-dependence of the fine structure map contraction in T4 phage is studied in two-factor crosses between rIIB mutants separated by indicator distances. The genetic intervals, which were short as compared with mean length of the heteroduplex region in hybrid DNA molecules but which exceeded the length of the DNA strand involved in a single correction event, were selected as indicator ones. On the basis of a deviation of measured frequencies from additivity (map contraction) the marker-specific frequencies of wild type recombinants arising as a result of correction to the wild type (kappa (- leads to +)) were calculated. For the most of the marker studied both of the base substitution and frame shift type the frequencies kappa (- leads to +) have the values below 2.10(-4). In the case of three most highly corrected frame shift markers with kappa (- leads to +) being 14.10(-4)--17.10(-4), about ten percent of all mismatched regions are corrected to the wild type.     

The leading routes and factors in the transmission of the causative agent of campylobacteriosis under current conditions]

Epidemiological investigations carried out at the foci of Campylobacter infection in Moscow and the Moscow region in 1987-1990 demonstrated that Campylobacter infection was recently registered as sporadic cases in a few foci. The alimentary route of the transmission of this infection was the main factor of its spread. A high role of everyday contacts in the spread of this infection was noted. The possibility for outbreaks and sporadic cases of Campylobacter infection to be masked by very frequently occurring associations of these bacteria is discussed.     

The effect of finoptin on the metabolism and pharmacological action of cyclophosphane in vivo and in vitro

Phynoptin (Ph) and cyclophosphamide (CP) gave rise to a type I spectral changes with liver microsomal fraction. KS were 15 microM and 2150 microM, respectively. Ph increases the concentration of NBP product(s) of CP and acrolein in the blood plasma of animals. Ph increases a toxicity of CP. LD50 was 388.0 +/- 13.9 mg/kg for CP and LD50 was 342.8 +/- 16.9 mg/kg for CP in combination with Ph. Ph changes a therapeutic action of CP in mice with hemocytoblastosis La. Pharmacokinetic interactions have been demonstrated between calcium antagonists Ph and CP.     

Interaction of acridine orange with teichoic acids of Staphylococcus aureus]

Teichoic acids of the cell wall of Staphylococcus aureus 209-P belong to the class of ribitol teichoic acids and can bind actively the molecules or acridine orange, changing its optical properties. The intravital fluorochromy of the cells with acridine orange may be caused by the sorption of its molecules by the teichoic acids of the cell wall.