全文获取类型
收费全文 | 7877篇 |
免费 | 718篇 |
国内免费 | 6篇 |
出版年
2023年 | 36篇 |
2022年 | 101篇 |
2021年 | 208篇 |
2020年 | 112篇 |
2019年 | 117篇 |
2018年 | 158篇 |
2017年 | 125篇 |
2016年 | 238篇 |
2015年 | 384篇 |
2014年 | 436篇 |
2013年 | 501篇 |
2012年 | 602篇 |
2011年 | 646篇 |
2010年 | 434篇 |
2009年 | 360篇 |
2008年 | 473篇 |
2007年 | 461篇 |
2006年 | 408篇 |
2005年 | 435篇 |
2004年 | 400篇 |
2003年 | 385篇 |
2002年 | 379篇 |
2001年 | 67篇 |
2000年 | 40篇 |
1999年 | 76篇 |
1998年 | 100篇 |
1997年 | 67篇 |
1996年 | 59篇 |
1995年 | 50篇 |
1994年 | 62篇 |
1993年 | 59篇 |
1992年 | 40篇 |
1991年 | 33篇 |
1990年 | 51篇 |
1989年 | 27篇 |
1988年 | 28篇 |
1987年 | 19篇 |
1986年 | 31篇 |
1985年 | 33篇 |
1984年 | 35篇 |
1983年 | 28篇 |
1982年 | 32篇 |
1981年 | 25篇 |
1980年 | 23篇 |
1978年 | 23篇 |
1977年 | 22篇 |
1976年 | 24篇 |
1975年 | 21篇 |
1973年 | 21篇 |
1970年 | 15篇 |
排序方式: 共有8601条查询结果,搜索用时 156 毫秒
91.
Kinley Ragan Jan Schipper Heather L. Bateman Sharon J. Hall 《The Journal of wildlife management》2023,87(1):e22322
Wildlife populations in semi-arid regions are increasingly challenged by human activities and dependent on the connectivity of riparian corridors for access to surface water. The Madrean Archipelago is a biodiversity hotspot along the arid United States–Mexico borderlands that support both Neotropical and Nearctic wildlife. Infrastructure development (e.g., the border wall and the expansion of Mexican Federal Highway 2) in this region inhibits wildlife movement along the transnational mountain archipelago by disconnecting habitat. To explore the relationship between habitat variables and mammal use of riparian corridors in northern Sonora, Mexico, we collected data from 19 motion-sensitive cameras between October 2018 and April 2019 and used single-season occupancy models and Royle-Nichols abundance estimation models to analyze our data. We recorded 21 species of mammals, including the first sighting of jaguar (Panthera onca) in this region in 25 years. River characteristics (distance from river, riparian corridor width, water availability), remoteness (distance from highway, productivity, elevation), and topographic variety (vertical elevation difference) influenced patterns of occupancy probability and estimated abundance of mammals >1 kg, but the strength and direction of these relationships varied by species. Additionally, intermittently wet desert washes were comparable in species richness to the perennial system. These results highlight the importance of examining physical and biological aspects of habitat. This is especially true when identifying corridors where mitigation structures should be placed to improve wildlife connectivity in biodiversity hotspots like the Madrean Archipelago and semi-arid ecosystems worldwide. 相似文献
92.
催产时鳝卵磷酸酶活性的变化及其与排卵的关系 总被引:2,自引:0,他引:2
本实验采用Gomori组织化学法和磷酸苯二钠法,分别检测黄鳝卵ALP、ACP的细胞定位和活性变化。结果表明,催产后72h,ALP活性显著高于临近注射时(Oh),催产后48h和96h(p<0.05);临产前(催产后96h),ACP活性极显著高于试验组其它各时程(p<0.01)。提示ALP和ACP活性增强与卵母细胞成熟、排放有直接关系。本研究为探索排卵机制提供资料。 相似文献
93.
Coelho-Rocha Nina Dias de Jesus Luís Cláudio Lima Barroso Fernanda Alvarenga Lima da Silva Tales Fernando Ferreira Enio Gonçalves José Eduardo dos Santos Martins Flaviano de Oliveira Carvalho Rodrigo Dias Barh Debmalya Azevedo Vasco Ariston de Carvalho 《Probiotics and antimicrobial proteins》2023,15(1):160-174
Probiotics and Antimicrobial Proteins - Beneficial effects of Lactiplantibacillus plantarum strains have been widely reported. Knowing that the effects of probiotic bacteria are strain-dependent,... 相似文献
94.
Edward J. Klekowski Jr. Nina Kazarinova-Fukshansky Leonid Fukshansky 《American journal of botany》1989,76(2):185-195
It is an axiom in biology that genes control the ontogeny and ultimately the final form of an organism. In plants a given morphological form can often arise through more than one ontogenetic pattern of cell divisions. Different ontogenetic patterns have different properties with regard to the final age in cell divisions of the initials in the meristems for a given morphological form. If mutation per genome per cell division is an important biological metric, then since the age of a cell in cell divisions is a function of ontogeny, the cellular ontogeny will influence the degree of mutation-loading in meristematic initials. Thus, ontogeny and form may affect the genes (by promoting or lessening genomic stasis) as well as, of course, being determined by the genes. This paper explores mathematically the relationship between different patterns of cell division and mutation-loading. 相似文献
95.
The aims of this study were to investigate the diet and relative abundance of fruit bats in a lowland Malaysian rain forest and to test the hypothesis that the local assemblage structure of fruit bats varies significantly over time in relation to the availability of food. In total, 352 fruit bats of eight species were captured during 72,306 m2 mist‐net hours of sampling between February 1996 and September 1999. Three species of fruit bats (Balionycteris maculita, Chironax melanocephalus, and Cynopterus brachyotis) that fed on a wide range of “steady state” and “big bang” food resources were captured continuously throughout the study period, with no significant variation in capture rates over time. In contrast, five species that fed exclusively or almost exclusively on “big bang” food resources were sampled intermittently, with significant temporal variation in the capture rates of two species (Cynopterus horsfieldi and Megaerops ecaudatus). Significant variation in the capture rates of the remaining three species (Dyacopterus spadiceus, Eonycteris spelaea, and Rousettus amplexicaudatus) could not be detected due to small sample sizes. Since ephemeral “big bang” food resources were only sporadically available within the study area and were associated with large canopy trees and strangler figs, these results suggest that food abundance, or the availability of specific food items, may be important factors limiting local fruit bat species diversity in old‐growth Paleotropical rain forest. Thus, only three fruit bat species were locally resident within the forest throughout the study period. Therefore, further studies on the ranging behavior and habitat requirements of Malaysian fruit bats are required to assess the adequacy of existing reserves and protected areas. 相似文献
96.
A transposon insertion in the Arabidopsis SSR16 gene causes an embryo-defective lethal mutation 总被引:15,自引:3,他引:12
Ryuji Tsugeki Elena Z. Kochieva Nina V. Fedoroff 《The Plant journal : for cell and molecular biology》1996,10(3):479-489
The SSR16 gene of Arabidopsis has been identified as a gene encoding a ribosomal protein S16 homolog through analysis of a transposon insertion mutation. The insertion mutation is lethal, arresting embryonic development at approximately the transition from the globular to the heart stage of embryonic development. Co-segregation of the mutant phenotype with the transposon-borne drug-resistance marker and loss of the inserted transposon concomitant with phenotypic reversion provided evidence that the transposon had caused the mutation. Sequences flanking the insertion site were amplified from DNA of viable heterozygotes by thermal asymmetric interlaced (TAIL) PCR. The amplified fragment flanking the 3' end of the inserted element was sequenced and found to be identical to an Arabidopsis expressed sequence tag (EST). The EST, in turn, contained a coding sequence homologous to the ribosomal protein S16 (RPS16) of bacteria such as Escherichia coli, Bacillus subtilis and Salmonella typhimurium , as well as Neurospora crassa mitochondria and higher plant plastids. Thus the gene identified by the embryo-defective lethal insertion mutation encodes an RPS16 homolog and has been designated the SSR16 gene. 相似文献
97.
Sharon L. Gursky 《International journal of primatology》1995,16(1):843-853
I present quantitative and qualitative data on infant caretaking behaviors collected during a preliminary field study of spectral
tarsiers (Tarsius spectrum),in a northern Sulawesi rain forest. The primary goal of the study is to identify the basic pattern of infant care in this
species. I studied tarsiers at Tangkoko-Dua Saudara Nature Reserve in Sulawesi, Indonesia, from May to July 1992. I observed
two infants, from two groups for a total of 96 hr using focal follows. During individual focal follows, ranging from 20 min
to 6 hr, I recorded behaviors at 5-min intervals. I also recorded distances of group members relative to the infant at 5-min
intervals. I subsampled the data at 35-min intervals to control for statistical autocorrelation between data points. Infants
were alone between 40 and 50% of the time. The two subadults were more frequently in proximity to the infant than the adult
males, the nonmatemal adult female, or the mothers were. This pattern of the subadults maintaining proximity to the infant
continued when the mothers were absent. These results suggest that subadults may be guarding or babysitting infants. It is
also possible that subadults are not traveling as far from the sleeping site as adults do and are therefore more likely to
be found in association with the infant. 相似文献
98.
99.
Assessment of microinjection for introducing DNA into uninuclear microspores of rapeseed 总被引:3,自引:0,他引:3
Elizabeth Jones-Villeneuve Bin Huang Isabelle Prudhomme Sharon Bird Roger Kemble Jiro Hattori Brian Miki 《Plant Cell, Tissue and Organ Culture》1995,40(1):97-100
Approximately 2,000 embryogenic uninuclear microspores of rapeseed (Brassica napus) cv. Topas were intranuclearly injected with a chimaeric -glucuronidase (Escherichia coli Uid A) gene. Stable integration had not occurred among 55 plants that were regenerated. Coinjection of the dye Lucifer Yellow and detection of injected DNA by the polymerase chain reaction revealed high frequencies of transfer. However, the amount of DNA injected was less than 20 copies, which may have been insufficient for stable transformation of microspores.Abbreviations PCR
polymerase chain reaction
- GUS
-glucuronidase 相似文献
100.