全文获取类型
收费全文 | 8730篇 |
免费 | 494篇 |
国内免费 | 6篇 |
专业分类
9230篇 |
出版年
2023年 | 78篇 |
2022年 | 122篇 |
2021年 | 258篇 |
2020年 | 153篇 |
2019年 | 171篇 |
2018年 | 235篇 |
2017年 | 217篇 |
2016年 | 273篇 |
2015年 | 359篇 |
2014年 | 407篇 |
2013年 | 570篇 |
2012年 | 584篇 |
2011年 | 565篇 |
2010年 | 337篇 |
2009年 | 279篇 |
2008年 | 392篇 |
2007年 | 352篇 |
2006年 | 335篇 |
2005年 | 333篇 |
2004年 | 276篇 |
2003年 | 225篇 |
2002年 | 232篇 |
2001年 | 202篇 |
2000年 | 179篇 |
1999年 | 164篇 |
1998年 | 60篇 |
1997年 | 59篇 |
1996年 | 65篇 |
1995年 | 53篇 |
1994年 | 60篇 |
1993年 | 39篇 |
1992年 | 119篇 |
1991年 | 119篇 |
1990年 | 91篇 |
1989年 | 96篇 |
1988年 | 88篇 |
1987年 | 90篇 |
1986年 | 87篇 |
1985年 | 93篇 |
1984年 | 83篇 |
1983年 | 50篇 |
1982年 | 48篇 |
1981年 | 44篇 |
1980年 | 48篇 |
1979年 | 78篇 |
1978年 | 58篇 |
1977年 | 49篇 |
1976年 | 42篇 |
1974年 | 39篇 |
1973年 | 44篇 |
排序方式: 共有9230条查询结果,搜索用时 0 毫秒
71.
S D Idriss R B Pilz V S Sharma G R Boss 《Biochemical and biophysical research communications》1992,183(1):312-320
We have purified the soluble form of guanylate cyclase from human placenta greater than 2400-fold. The enzyme shared several characteristics with the enzyme purified from other sources including molecular mass and subunit composition, activation by divalent cations, inhibition by ATP and Michaelis constants. The enzyme, however, had a lower absorption maximum in the Soret region (417 +/- 1 nm) than the enzyme from other sources and was activated only one-fifth as much by nitric oxide as the bovine lung enzyme. It appears that the heme prosthetic group in the human placental enzyme may be hexa-coordinate and in the bovine lung enzyme the heme group may be penta-coordinate. 相似文献
72.
73.
Forest density expressing the stocking status constitutes the major stand physiognomic parameter of Indian forest. Density
and age are often taken as surrogate to structural and compositional changes that occur with the forest succession. Satellite
remote sensing spectral response is reported to provide information on structure and composition of forest stands. The various
vegetation indices are also correlated with forest canopy closure. The paper presents a three way crown density model utilizing
the vegetation indices viz., advanced vegetation index, bare soil index and canopy shadow index for classification of forest
crown density. The crop and water classes which could not be delineated by the model were finally masked from normalized difference
vegetation index and TM band 7 respectively. The rule based approach has been implemented for land use and forest density
classification. The broad land cover classification accuracy has been found to be 91.5%. In the higher forest density classes
the classification accuracy ranged between 93 and 95%, whereas in the lower density classes it was found to be between 82
and 85%. 相似文献
74.
S. Gulati M. Khullar B. K. Sharma N. K. Ganguly 《Molecular and cellular biochemistry》1996,156(1):37-42
Intracellular free Ca2+ concentration has been shown to be elevated in platelets of patients with essential hypertension. This study was designed to characterize Ca2+-pump activity of the platelet membranes (surface and intracellular) in these patients. A double-blind study was carried out. Untreated and treated (on R-blockers) essential hypertensives were studied in comparison with normotensive control subjects. First degree blood relatives of essential hypertensives were also studied. The Ca2+-activation kinetics of the enzyme showed a significant decrease in the Vmax. (for the plasma- and intracellular membranes) and Km (for the intracellular membranes) in the essential hypertensive patients. Increased platelet membrane cholesterol content was observed in these patients. Lowered Ca2+-efflux by Ca2+-ATPase may lead to elevated intracellular free Ca2+-levels in platelets of essential hypertensives. A lowered Ca2+-ATPase activity may emerge as a marker for essential hypertension. 相似文献
75.
Poloxamer 188 decreases susceptibility of artificial lipid membranes to electroporation. 总被引:1,自引:0,他引:1 下载免费PDF全文
The effect of a nontoxic, nonionic block co-polymeric surface active agent, poloxamer 188, on electroporation of artificial lipid membranes made of azolectin, was investigated. Two different experimental protocols were used in our study: charge pulse and voltage clamp. For the charge pulse protocol, membranes were pulsed with a 10-micronsecond rectangular voltage waveform, after which membrane voltage decay was observed through an external 1-M omega resistance. For the voltage clamp protocol the membranes were pulsed with a waveform that consisted of an initial 10-microsecond rectangular phase, followed by a negative sloped ramp that decayed to zero in the subsequent 500 microseconds. Several parameters characterizing the electroporation process were measured and compared for the control membranes and membranes treated with 1.0 mM poloxamer 188. For both the charge pulse and voltage clamp experiments, the threshold voltage (amplitude of initial rectangular phase) and latency time (time elapsed between the end of rectangular phase and the onset of membrane electroporation) were measured. Membrane conductance (measured 200 microseconds after the initial rectangular phase) and rise time (tr; the time required for the porated membrane to reach a certain conductance value) were also determined for the voltage clamp experiments, and postelectroporation time constant (PE tau; the time constant for transmembrane voltage decay after onset of electroporation) for the charge pulse experiments. The charge pulse experiments were performed on 23 membranes with 10 control and 13 poloxamer-treated membranes, and voltage pulse experiments on 49 membranes with 26 control and 23 poloxamer-treated membranes. For both charge pulse and voltage clamp experiments, poloxamer 188-treated membranes exhibited a statistically higher threshold voltage (p = 0.1 and p = 0.06, respectively), and longer latency time (p = 0.04 and p = 0.05, respectively). Also, poloxamer 188-treated membranes were found to have a relatively lower conductance (p = 0.001), longer time required for the porated membrane to reach a certain conductance value (p = 0.05), and longer postelectroporation time constant (p = 0.005). Furthermore, addition of poloxamer 188 was found to reduce the membrane capacitance by approximately 4-8% in 5 min. These findings suggest that poloxamer 188 adsorbs into the lipid bilayers, thereby decreasing their susceptibility to electroporation. 相似文献
76.
Transport of bacteria in porous media: II. A model for convective Transport and growth 总被引:1,自引:0,他引:1
A model is presented for the coupled processes of bacterial growth and convective transport of bacteria has been modeled using a fractional flow approach. The various mechanisms of bacteria retention can be incorporated into the model through selection of an appropriate shape of the fractional flow curve. Permeability reduction due to pore plugging by bacteria was simulated using the effective medium theory. In porous media, the rates of transport and growth of bacteria, the generation of metabolic products, and the consumption of nutrients are strongly coupled processes. Consequently, the set of governing conservation equations form a set of coupled, nonlinear partial differential equations that were solved numerically. Reasonably good agreement between the model and experimental data has been obtained indicating that the physical processes incorporated in the model are adequate. The model has been used to predict the in situ transport and growth of bacteria, nutrient consumption, and metabolite production. It can be particularly useful in simulating laboratory experiments and in scaling microbial-enhanced oil recovery or bioremediation processes to the field. (c) 1994 John Wiley & Sons, Inc. 相似文献
77.
Xanthomonas campestris pv. glycines , (Xcg), the causative agent of the bacterial pustule disease of soybean was isolated and characterized. On susceptible soybean the pathogenic isolates displayed characteristic chlorotic lesions around the site of infection within 48 h of inoculation. The pathogenic isolates were found to contain two cryptic plasmids. A smaller plasmid of 1.5 kb and a larger one of size about 25 kb. SDS-PAGE profile of the soluble proteins of the pathogenic isolatess, howed a different pattern compared to that of the non-pathogenic isolates. 相似文献
78.
Summary The susceptibility of 50 drug resistant strains of Escherichia coli of human gut was determined against ciprofloxacin, acridine orange (AO) and sodium dodecyl sulphate (SDS). Curing efficacy of these agents were worked out at subminimal inhibitory concentrations. Ciprofloaxacin was found a better curing agent for E coli R-plasmids, eliminating R-factors from 48% of the strains followed by SDS and AO which eliminated 24% and 20% of the drug resistance determinants, respectively. Elimination of R-plasmids was found dependent on the concentrations of curing agents and nature of R-plasmids. 相似文献
79.
80.
Bahman Tabaraie Bal Krishan Sharma Praveen Rishi nee Sharma Rakesh Sehgal Nirmal Kumar Ganguly 《Microbiology and immunology》1994,38(7):553-559
Porins were prepared from smooth strain of Salmonella typhi 0–901 and chemotype of rough mutant of S. typhimurium Ra-30. Mice were immunized with both the porin preparations in different groups and challenged with S. typhimurium LT2–71 and S. enteritidis SH-1269. Porin immunized mice showed significant protection (P <0.01) against challenge with homologous as well as heterologous strains. Hence, the use of porins may be attempted in future to protect against salmonellosis. 相似文献