全文获取类型
收费全文 | 2372篇 |
免费 | 213篇 |
国内免费 | 6篇 |
专业分类
2591篇 |
出版年
2022年 | 19篇 |
2021年 | 37篇 |
2020年 | 19篇 |
2019年 | 23篇 |
2018年 | 38篇 |
2017年 | 25篇 |
2016年 | 57篇 |
2015年 | 115篇 |
2014年 | 122篇 |
2013年 | 139篇 |
2012年 | 210篇 |
2011年 | 155篇 |
2010年 | 96篇 |
2009年 | 80篇 |
2008年 | 116篇 |
2007年 | 116篇 |
2006年 | 127篇 |
2005年 | 93篇 |
2004年 | 97篇 |
2003年 | 87篇 |
2002年 | 66篇 |
2001年 | 73篇 |
2000年 | 59篇 |
1999年 | 73篇 |
1998年 | 28篇 |
1997年 | 22篇 |
1996年 | 12篇 |
1995年 | 13篇 |
1994年 | 19篇 |
1993年 | 15篇 |
1992年 | 34篇 |
1991年 | 38篇 |
1990年 | 31篇 |
1989年 | 20篇 |
1988年 | 21篇 |
1987年 | 20篇 |
1986年 | 26篇 |
1985年 | 26篇 |
1984年 | 13篇 |
1983年 | 19篇 |
1982年 | 19篇 |
1981年 | 14篇 |
1980年 | 16篇 |
1979年 | 20篇 |
1978年 | 18篇 |
1976年 | 17篇 |
1975年 | 15篇 |
1974年 | 11篇 |
1973年 | 15篇 |
1972年 | 13篇 |
排序方式: 共有2591条查询结果,搜索用时 21 毫秒
101.
KCNQ1 variants associate with hypertension in type 2 diabetes and affect smooth muscle contractility in vitro 下载免费PDF全文
Kuo‐Chin Huang Te‐Mao Li Xiang Liu Jin‐Hua Chen Wen‐Kuei Chien Yi‐Tzone Shiao Hsinyi Tsang Ting‐Hsu Lin Chiu‐Chu Liao Shao‐Mei Huang Ju‐Pi Li Cheng‐Wen Lin Jung‐Chun Lin Chih‐Chien Lin Chih‐Ho Lai Chi‐Fung Cheng Wen‐Miin Liang Chien‐Hui Hung Ching‐Chu Chen Ying‐Ju Lin Fuu‐Jen Tsai 《Journal of cellular physiology》2017,232(12):3309-3316
102.
Bullatacin, isolated from the fruit of Annona atemoya, is one of the most potentially effective antitumor annonaceous acetogenins. Bullatacin was studied here for its ability to inhibit the proliferation of 2.2.15 cells, hepatitis B virus (HBV) DNA transfected human hepatocarcinoma cell line. It was found that bullatacin induced cytotoxicity of 2.2.15 cells in a time- and dose-dependent manner. Fifty percent effective dose (ED50) on day 1 of exposure to bullatacin were 7.8 +/- 2.5 nM for 2.2.15 cells. [3H]-Thymidine incorporation assays showed almost the same results. Bullatacin-treatment also reduced concentrations of hepatitis B surface antigen (HBsAg) in the cultured medium released from 2.2.15 cells, coincident with the decrease in the cell proliferation. Analysis of mophological changes of bullatacin-treated 2.2.15 by inverted phase-contrast microscope and eletron microscopy revealed a possible model of action for bullatacin to inhibit proliferation of 2.2.15 cells by inducing apoptosis. Most of the bullatacin-induced cell death was found to be due to apoptosis, as determined by double staining with fluorescein-isothiocyanate (FITC)-labeled annexin V and propidium iodide (PI). 相似文献
103.
Chiu Chuen Onn Sumiani Yusoff 《The International Journal of Life Cycle Assessment》2010,15(9):985-993
Background, aim, and scope
Life Cycle Assessment (LCA) is an emerging supporting tool designed to help practitioner in systematically assessing the environmental performance of selected product’s life cycle. A product’s life cycle includes the extraction of raw materials, production, and usage, and ends with waste treatment or disposal. Life cycle impact assessment (LCIA) as a part of LCA is a method used to derive the environmental burdens from selected product’s stages. LCIA is structured in classification, characterization, normalization and weighting. Presently most of the LCIA practices use European database to establish the characterization, normalization and weighting value. However, using these values for local LCA practice might not be able to reflect the actual Malaysian’s environmental scenario. The aim of this study is to create a Malaysian version of normalization and weighting value using the pollution database within Malaysia. 相似文献104.
Three-dimensional structure of rotavirus 总被引:40,自引:0,他引:40
105.
106.
Intracerebral hemorrhage (ICH) is associated with high mortality and disability, and hyperglycemia worsens the clinical and neurological outcomes of patients with ICH. In this study, we utilized proteomic approaches to investigate the role of hyperglycemia in ICH. Hyperglycemia was induced by intraperitoneal injection of streptozotocin (STZ) in adult Sprague-Dawley male rats; ICH was induced by stereotaxic infusion of collagenase/heparin into the right striatum. It was observed that the size of induced hemorrhage was significantly larger in the hyperglycemic group (n=6 in each group). On the first day after ICH, an apparent decrease in the bilateral grasp was also observed for the lesioned hyperglycemic rats compared with normoglycemic ones. When employing 2-DE and MS to examine the proteomes of perihematomal and control regions in individual hyperglycemic and normoglycemic rats, eight differentially expressed protein targets were identified. Most noteworthy, in response to ICH significant increase of albumin was ubiquitously observed in the brains of normoglycemic rats but not in the brains of hyperglycemic rats. Coincidentally, more significant neuronal apoptosis were found in the perihematomal regions of hyperglycemic rats. These observations described suggest the protection role of albumin in acute stage of ICH, which may be dependent on different blood sugar levels. 相似文献
107.
108.
Tzu-Ching Huang Pu-Rong Chiu Wen-Tsan Chang Bau-Shan Hsieh Yu-Ci Huang Hsiao-Ling Cheng Li-Wen Huang Yu-Chen Hu Kee-Lung Chang 《Apoptosis : an international journal on programmed cell death》2018,23(3-4):226-236
Epirubicin is an anthracycline and is widely used in tumor treatment, but has toxic and undesirable side effects on wide range of cells and hematopoietic stem cells (HSC). Osteoblasts play important roles in bone development and in supporting HSC differentiation and maturation. It remains unknown whether epirubicin-induced bone loss and hematological toxicity are associated with its effect on osteoblasts. In primary osteoblast cell cultures, epirubicin inhibited cell growth and decreased mineralization. Moreover, epirubicin arrested osteoblasts in the G2/M phase, and this arrest was followed by apoptosis in which both the extrinsic (death receptor-mediated) and intrinsic (mitochondrial-mediated) apoptotic pathways were evoked. The factors involved in the extrinsic apoptotic pathway were increased FasL and FADD as well as activated caspase-8. Those involved in the intrinsic apoptotic pathway were decreased Bcl-2; increased reactive oxygen species, Bax, cytochrome c; and activated caspase-9 and caspase-3. These results demonstrate that epirubicin induced osteoblast apoptosis through the extrinsic and intrinsic apoptotic pathways, leading to the destruction of osteoblasts and consequent lessening of their functions in maintaining bone density and supporting hematopoietic stem cell differentiation and maturation. 相似文献
109.
110.