Fuzzy logic feedback control for fed-batch enzymatic hydrolysis of lignocellulosic biomass

A fuzzy logic feedback control system was developed for process monitoring and feeding control in fed-batch enzymatic hydrolysis of a lignocellulosic biomass, dilute acid-pretreated corn stover. Digested glucose from hydrolysis reaction was assigned as input while doser feeding time and speed of pretreated biomass were responses from fuzzy logic control system. Membership functions for these three variables and rule-base were created based on batch hydrolysis data. The system response was first tested in LabVIEW environment then the performance was evaluated through real-time hydrolysis reaction. The feeding operations were determined timely by fuzzy logic control system and efficient responses were shown to plateau phases during hydrolysis. Feeding of proper amount of cellulose and maintaining solids content was well balanced. Fuzzy logic proved to be a robust and effective online feeding control tool for fed-batch enzymatic hydrolysis.     

Methylmercury toxicity: amelioration by selenium and water‐soluble chelators as N‐acetyl cysteine and dithiothreitol

The protective potential of chelators, i.e. N‐acetyl cysteine (0.6 mg /kg, intraperitoneally) and dithiothreitol (15.4 mg kg^?1, intraperitoneally) with selenium (0.5 mg kg^?1, pre‐oral) were evaluated individually and in combination against methylmercury‐induced biochemical alterations and oxidative stress consequences. Forty‐two male Sprague–Dawley rats were exposed with methylmercury (1.5 mg kg^?1, pre‐oral) daily for 21 days followed by different treatments for five consecutive days. Administration of methylmercury caused significant enhancement in the release of transaminases, alkaline phosphatases and lactate dehydrogenases in serum. A significant increased was observed in lipid peroxidation level with a concomitant decreased in glutathione content after methylmercury exposure in liver, kidney and brain. Hepatic microsomal drug metabolizing enzymes (aniline hydroxylase and amidopyrine N‐demethylase) of cytochrome p4502E₁ showed sharp depletion after methylmercury exposure. Alterations in histological changes in liver, kidney and brain were also noted in methylmercury administered group. All treated groups showed recovery pattern, but the combined treatments with N‐acetyl cysteine and dithiothreitol in combination with selenium were more effective than that with either alone treatments in recovering blood biochemical changes after methylmercury toxicity. In conclusion, the results demonstrated that combination therapy may recover all blood biochemical alterations and offer maximum protection against methylmercury‐induced toxicity. Copyright © 2014 John Wiley & Sons, Ltd.     

A pregnane ester diglycoside from periploca calophylla

A new pregnane ester diglycoside of ornogenin named as plocinine was isolated from the dried twigs of Periploca calophylla. On the basis of chemical and spectroscopic evidences its structure was established as 12,20-di-O-cinnamoyl sarcostin-3-O-α-L-oleandropyranosyl (1 → 4)-O-α-L-oleandropyranoside.     

Sufficient-completeness, ground-reducibility and their complexity

Summary The sufficient-completeness property of equational algebraic specifications has been found useful in providing guidelines for designing abstract data type specifications as well as in proving inductive properties using the induction-less-induction method. The sufficient-completeness property is known to be undecidable in general. In an earlier paper, it was shown to be decidable for constructor-preserving, complete (canonical) term rewriting systems, even when there are relations among constructor symbols. In this paper, the complexity of the sufficient-completeness property is analyzed for different classes of term rewriting systems. A number of results about the complexity of the sufficient-completeness property for complete (canonical) term rewriting systems are proved: (i) The problem is co-NP-complete for term rewriting systems with free constructors (i.e., no relations among constructors are allowed), (ii) the problem remains co-NP-complete for term rewriting systems with unary and nullary constructors, even when there are relations among constructors, (iii) the problem is provably in almost exponential time for left-linear term rewriting systems with relations among constructors, and (iv) for left-linear complete constructor-preserving rewriting systems, the problem can be decided in steps exponential innlogn wheren is the size of the rewriting system. No better lower-bound for the complexity of the sufficient-completeness property for complete (canonical) term rewriting system with nonlinear left-hand sides is known. An algorithm for left-linear complete constructor-preserving rewriting systems is also discussed. Finally, the sufficient-completeness property is shown to be undecidable for non-linear complete term rewriting systems with associative functions. These complexity results also apply to the ground-reducibility property (also called inductive-reducibility) which is known to be directly related to the sufficient-completeness property.Some of the results in this paper were reported in a paper titled Complexity of Sufficient-Completeness presented at theSixth Conf. on Foundations of Software Technology and Theoretical Computer Science, New Delhi, India, Dec. 1986. The term quasi-reducibility is replaced in this paper by ground-reducibility as the latter seems to convey a lot more about the concept than the former.Partially supported by the National Science Foundation Grant nos. CCR-8408461 and CCR-8906678Partially supported by the National Science Foundation Grant nos. CCR-8408461 and CCR-9009414Partially supported by the National Science Foundation Grant no. DCR-8603184     

Simulation Studies of Photoacoustic Response from Gold-Silica Core-Shell Nanoparticles

Metal nanoparticles especially of noble metals are used as an exogenous contrast agent for biomedical photoacoustic (PA) imaging in the tissue transmission window extending from visible to near infrared 700–1100 nm band. Different geometrical configurations of gold and silver nanoparticles like spherical core-shell, nanorod, and nanocages are promising candidates for thermoplasmonics, photothermal therapy, photothermal imaging, and photoacoustic imaging. In the current study, we simulated the photoacoustic response of gold and silica core-shell nanoparticle in water medium. Finite element simulations were carried out to study the spectral absorption response and effect of nanosecond laser pulse excitation on the spatial/temporal temperature as well as photoacoustic pressure variations of different core-shell geometry of nanoparticle. We have optimized the dimensions of gold nanosphere, gold-silica, and silica-gold core-shell geometries for optimum photoacoustic conversion efficiency. Further, the effect of shell thickness on the pulse photoacoustic signals for core-shell gold-silica and silica-gold nanoparticle has been studied. We concluded that silica-gold core-shell nanoparticles possess better photoacoustic conversion efficiency in comparison to gold nanosphere and gold-silica core-shell geometries. The prime aim of this study is to design efficient nano-probes for photoacoustic imaging, photoacoustic tomography, photothermal therapy, and drug delivery.     

Paradigm for disease deconvolution in rare neurodegenerative disorders in Indian population: insights from studies in cerebellar ataxias

Cerebellar ataxias are a group of rare progressive neurodegenerative disorders with an average prevalence ranges from 4.8 to 13.8 in 100,000 individuals. The inherited disorders affect multiple members of the families, or a community that is endogamous or consanguineous. Presence of more than 3000 mutations in different genes with overlapping clinical symptoms, genetic anticipation and pleiotropy, as well as incomplete penetrance and variable expressivity due to modifiers pose challenges in genotype–phenotype correlation. Development of a diagnostic algorithm could reduce the time as well as cost in clinicogenetic diagnostics and also help in reducing the economic and social burden of the disease. In a unique research collaboration spanning over 20 years, we have been able to develop a paradigm for studying cerebellar ataxias in the Indian population which would also be relevant in other rare diseases. This has involved clinical and genetic analysis of thousands of families from diverse Indian populations. The extensive resource on ataxia has led to the development of a clinicogenetic algorithm for cost-effective screening of ataxia and a unique ataxia clinic in the tertiary referral centre in All India Institute of Medical Sciences. Utilizing a population polymorphism scanning approach, we have been able to dissect the mechanisms of repeat instability and expansion in many ataxias, and also identify founders, and trace the mutational histories in the Indian population. This provides information for genetic testing of at—risk as well as protected individuals and populations. To dissect uncharacterized cases which comprises more than 50% of the cases, we have explored the potential of next-generation sequencing technologies coupled with the extensive resource of baseline data generated in-house and other public domains. We have also developed a repository of patient-derived peripheral blood mononuclear cells, lymphoblastoid cell lines and neuronal lineages (derived from iPSCs) for ascribing functionality to novel genes/mutations. Through integrating these technologies, novel genes have been identified that has broadened the diagnostic panel, increased the diagnostic yield to over 75%, helped in ascribing pathogenicity to novel mutations and enabled understanding of disease mechanisms. It has also provided a platform for testing novel molecules for amelioration of pathophysiological phenotypes. This review through a perspective on CAs suggests a generic paradigm from diagnostics to therapeutic interventions for rare disorders in the context of heterogeneous Indian populations.