全文获取类型
收费全文 | 10719篇 |
免费 | 1048篇 |
国内免费 | 3篇 |
出版年
2022年 | 92篇 |
2021年 | 191篇 |
2020年 | 137篇 |
2019年 | 141篇 |
2018年 | 173篇 |
2017年 | 160篇 |
2016年 | 243篇 |
2015年 | 410篇 |
2014年 | 460篇 |
2013年 | 604篇 |
2012年 | 630篇 |
2011年 | 681篇 |
2010年 | 439篇 |
2009年 | 401篇 |
2008年 | 566篇 |
2007年 | 548篇 |
2006年 | 482篇 |
2005年 | 470篇 |
2004年 | 432篇 |
2003年 | 398篇 |
2002年 | 405篇 |
2001年 | 183篇 |
2000年 | 221篇 |
1999年 | 182篇 |
1998年 | 127篇 |
1997年 | 105篇 |
1996年 | 90篇 |
1995年 | 87篇 |
1994年 | 86篇 |
1992年 | 130篇 |
1991年 | 113篇 |
1990年 | 128篇 |
1989年 | 100篇 |
1988年 | 144篇 |
1987年 | 100篇 |
1986年 | 86篇 |
1985年 | 117篇 |
1984年 | 90篇 |
1983年 | 98篇 |
1982年 | 102篇 |
1981年 | 69篇 |
1980年 | 67篇 |
1979年 | 74篇 |
1978年 | 78篇 |
1977年 | 89篇 |
1976年 | 77篇 |
1975年 | 74篇 |
1974年 | 83篇 |
1973年 | 67篇 |
1972年 | 70篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
11.
Amy M. LaFountain Shannon Cawley Steven J. Schwartz Richard O. Prum 《Archives of biochemistry and biophysics》2010,504(1):142-153
Recent advances in the fields of chromatography, mass spectrometry, and chemical analysis have greatly improved the efficiency with which carotenoids can be extracted and analyzed from avian plumage. Prior to these technological developments, Brush (1968) [1] concluded that the burgundy-colored plumage of the male pompadour Cotinga Xipholena punicea is produced by a combination of blue structural color and red carotenoids, including astaxanthin, canthaxanthin, isozeaxanthin, and a fourth unidentified, polar carotenoid. However, X. punicea does not in fact exhibit any structural coloration. This work aims to elucidate the carotenoid pigments of the burgundy color of X. punicea plumage using advanced analytical methodology. Feathers were collected from two burgundy male specimens and from a third aberrant orange-colored specimen. Pigments were extracted using a previously published technique (McGraw et al. (2005) [2]), separated by high-performance liquid chromatography (HPLC), and analyzed by UV/Vis absorption spectroscopy, chemical analysis, mass spectrometry, nuclear magnetic resonance (NMR), and comparison with direct synthetic products. Our investigation revealed the presence of eight ketocarotenoids, including astaxanthin and canthaxanthin as reported previously by Brush (1968) [1]. Six of the ketocarotenoids contained methoxyl groups, which is rare for naturally-occurring carotenoids and a novel finding in birds. Interestingly, the carotenoid composition was the same in both the burgundy and orange feathers, indicating that feather coloration in X. punicea is determined not only by the presence of carotenoids, but also by interactions between the bound carotenoid pigments and their protein environment in the barb rami and barbules. This paper presents the first evidence of metabolically-derived methoxy-carotenoids in birds. 相似文献
12.
Roy Ronen Glenn Tesler Ali Akbari Shay Zakov Noah A. Rosenberg Vineet Bafna 《PLoS genetics》2015,11(9)
Methods for detecting the genomic signatures of natural selection have been heavily studied, and they have been successful in identifying many selective sweeps. For most of these sweeps, the favored allele remains unknown, making it difficult to distinguish carriers of the sweep from non-carriers. In an ongoing selective sweep, carriers of the favored allele are likely to contain a future most recent common ancestor. Therefore, identifying them may prove useful in predicting the evolutionary trajectory—for example, in contexts involving drug-resistant pathogen strains or cancer subclones. The main contribution of this paper is the development and analysis of a new statistic, the Haplotype Allele Frequency (HAF) score. The HAF score, assigned to individual haplotypes in a sample, naturally captures many of the properties shared by haplotypes carrying a favored allele. We provide a theoretical framework for computing expected HAF scores under different evolutionary scenarios, and we validate the theoretical predictions with simulations. As an application of HAF score computations, we develop an algorithm (PreCIOSS: Predicting Carriers of Ongoing Selective Sweeps) to identify carriers of the favored allele in selective sweeps, and we demonstrate its power on simulations of both hard and soft sweeps, as well as on data from well-known sweeps in human populations. 相似文献
13.
14.
15.
16.
17.
E N Baramova J D Shannon J B Bjarnason J W Fox 《Archives of biochemistry and biophysics》1989,275(1):63-71
The proteolytic activity of four hemorrhagic metalloproteinases (Ht-a, c, d, and e) isolated from the venom of the Western diamondback rattlesnake (Crotalus atrox) was investigated using isolated extracellular matrix (ECM) proteins. We determined that all of the proteinases are capable of cleaving fibronectin, laminin, type IV collagen, nidogen (entactin), and gelatins. However, none of the proteinases were proteolytic against the interstitial collagen types I and III or type V collagen. With all of the substrates listed above Ht-c and Ht-d produced identical digestion patterns, as would be expected for these isoenzymes. With fibronectin, Ht-a produces a different ratio of products from Ht-c and Ht-d, while Ht-e produces a unique pattern of digestion. Ht-e and Ht-a produced nonidentical patterns with the laminin/nidogen preparation although some similarity was shared between them as well as with the Ht-c/d digestion pattern. Similar results were also observed for these proteinases with nidogen 150 as the substrate. The type IV collagen digestion patterns by Ht-e and Ht-a were similar to the pattern observed with Ht-c/d but differed by two bands. The digestion patterns of the three gelatins produced by the proteinases show differences between Ht-c and Ht-d when compared to Ht-e and Ht-a. This investigation clearly shows that several of the ECM proteins are efficiently digested by these toxins. The proteinases have some digestion sites in common but show differing specificities. In addition, the range of ECM proteins digested by these hemorrhagic proteinases is nearly identical to that demonstrated by the ECM proteinase stromelysin (MMP-3). From these data, and the knowledge of the roles these ECM proteins have in maintaining basement membrane structural/functional integrity, one can envision that the degradation of these ECM proteins could readily lead to loss of capillary integrity resulting in hemorrhage occurring at those sites. 相似文献
18.
Abstract The bifunctional T-protein (chorismate mutase-T: cyclohexadienyl dehydrogenase) of l -tyrosine biosynthesis was found to be present in all genera making up the enteric bacteria. The dehydrogenase component of the T-protein was active with both prephenate and l -arogenate, showing it to be a cyclohexadienyl dehydrogenase. The dehydrogenase component, but not the mutase component, of the T-protein was feedback-inhibited by l -tyrosine. Unlike some other bifunctional proteins, the T-protein has evolved recently and is not ubiquitous. However, once the biochemical specialization of bifunctionality becomes established, the results indicate that such character states are strongly conserved through evolutionary time. Thus, bifunctional proteins can provide particularly reliable markers for small (recent origin), intermediate, and large (ancient origin) phylogenetic clusters. 相似文献
19.
Marjorie Jones Roy W. Keenan 《Biochimica et Biophysica Acta (BBA)/General Subjects》1981,678(3):403-407
A procedure was developed for the detection of 2′,3′-cyclic nucleotide 3′-phosphohydrolase in myelin. This assay was sufficiently sensitive to detect the low levels of 2′,3′-cyclic nucleotide 3′-phosphohydrolase in human erythrocytes. The 2′,3′-cyclic nucleotide 3′-phosphohydrolase of human erythrocytes was determined to be exclusively associated with the inner (cytosolic) side of the membrane. Leaky ghostsand resealed ghosts were assayed for 2′,3′-cyclic nucleotide 3′-phosphohydrolase, (Ca2+/Mg2+-ATPase, and acetylcholinesterase activity, and the 2′,3′-cyclic nucleotide 3′-phosphohydrolase profile is the same as that of the (Ca2+/Mg2+)-ATPase, an established inner membrane maker. 相似文献
20.
The shikimate/arogenate pathway: Link between carbohydrate metabolism and secondary metabolism 总被引:2,自引:0,他引:2
Roy A. Jensen 《Physiologia plantarum》1986,66(1):164-168