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161.
Zhang Fan Wang Jiawen Lü Dongyuan Zheng Lu Shangguan Bing Gao Yuxin Wu Yi Long Mian 《Biomechanics and modeling in mechanobiology》2021,20(1):205-222
Biomechanics and Modeling in Mechanobiology - Human embryonic stem cells (hESCs) can differentiate to three germ layers within biochemical and biomechanical niches. The complicated mechanical... 相似文献
162.
Shaoyan Chang Li Wang Yunqian Guan Shaofang Shangguan Qingan Du Yang Wang Ting Zhang Prof. Yu Zhang 《Journal of cellular biochemistry》2013,114(7):1549-1558
Folate is thought to contribute to health and development by methylation regulation. Long interspersed nucleotide element‐1 (LINE‐1), which is regulated by methylation modification, plays an important role in sculpting the structure and function of genomes. Some studies have shown that folate concentration is related to LINE‐1 methylation. However, the direct association between LINE‐1 methylation and folate deficiency remains unclear. To explore whether folate deficiency directly induced LINE‐1 hypomethylation and to analyze the relationship between folate concentration and the LINE‐1 methylation level, mouse ESCs were treated with various concentrations of folate which was measured by chemiluminescent immunoassay, and the homocysteine content was detected by ELISA. LINE‐1 methylation was examined by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry at various time points. Concurrently, cell proliferation and differentiation were observed. The result showed that the intracellular folate decreases under folate‐deficient condition, conversely, homocysteine content increased gradually and there was a negatively correlated between them. Folate insufficiency induced LINE‐1 hypomethylation at the lowest levels in folate‐free group and moderate in folate‐deficient group, compared with that in the folate‐normal group at day 18. Moreover, LINE‐1 methylation level was positively correlated with folate content, and negatively correlated with homocysteine content. At corresponding time points, proliferation and differentiation of mouse ESCs showed no alteration in all groups. Our data indicated that folate deficiency affected the homeostasis of folate‐mediated one‐carbon metabolism, leading to reduced LINE‐1 methylation in mouse ESCs. This study provides preliminary evidence of folate deficiency affecting early embryonic development. J. Cell. Biochem. 114: 1549–1558, 2013. © 2013 Wiley Periodicals, Inc. 相似文献
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164.
Huang Jin-Quan Lin Jia-Ling Guo Xiao-Xiang Tian Xiu Tian Ye Shangguan Xiao-Xia Wang Ling-Jian Fang Xin Chen Xiao-Ya 《中国科学:生命科学英文版》2020,63(9):1297-1302
The reactive electrophilic species(RES), typically the molecules bearing α,β-unsaturated carbonyl group, are widespread in living organisms and notoriously known for their damaging effects. Many of the mycotoxins released from phytopathogenic fungi are RES and their contamination to cereals threatens food safety worldwide. However, due to their high reactivity, RES are also used by host organisms to synthesize specific metabolites. The evolutionary conserved glyoxalase(GLX) system scavenges the cytotoxic α-oxoaldehydes that bear RES groups, which cause host disorders and diseases. In cotton, a specialized enzyme derived from glyoxalase I(GLXI) through gene duplications and named as specialized GLXI(SPG), acts as a distinct type of aromatase in the gossypol pathway to transform the RES intermediates into the phenolic products. In this review, we briefly introduce the research progress in understanding the RES, especially the RES-type mycotoxins, the GLX system and SPG, and discuss their application potential in detoxification and synthetic biology. 相似文献
165.
Lu Chengrong Liu Huan Shangguan Wendan Chen Song Zhong Qingping 《Archives of microbiology》2021,203(1):125-135
Archives of Microbiology - Vibrio parahaemolyticus and Escherichia coli are two major foodborne pathogens. In this paper, the antibiofilm activities of the ethanol extract of cinnamon against these... 相似文献
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Zhida Wu Meiling Zuo Ling Zeng Kaisa Cui Bing Liu Chaojun Yan Li Chen Jun Dong Fugen Shangguan Wanglai Hu He He Bin Lu Zhiyin Song 《EMBO reports》2021,22(1)
Many cancer cells maintain enhanced aerobic glycolysis due to irreversible defective mitochondrial oxidative phosphorylation (OXPHOS). This phenomenon, known as the Warburg effect, is recently challenged because most cancer cells maintain OXPHOS. However, how cancer cells coordinate glycolysis and OXPHOS remains largely unknown. Here, we demonstrate that OMA1, a stress‐activated mitochondrial protease, promotes colorectal cancer development by driving metabolic reprogramming. OMA1 knockout suppresses colorectal cancer development in AOM/DSS and xenograft mice models of colorectal cancer. OMA1‐OPA1 axis is activated by hypoxia, increasing mitochondrial ROS to stabilize HIF‐1α, thereby promoting glycolysis in colorectal cancer cells. On the other hand, under hypoxia, OMA1 depletion promotes accumulation of NDUFB5, NDUFB6, NDUFA4, and COX4L1, supporting that OMA1 suppresses OXPHOS in colorectal cancer. Therefore, our findings support a role for OMA1 in coordination of glycolysis and OXPHOS to promote colorectal cancer development and highlight OMA1 as a potential target for colorectal cancer therapy. 相似文献
169.
Shangguan Lingfei Fang Xiang Jia Haifeng Chen Mengxia Zhang Kekun Fang Jinggui 《Physiology and Molecular Biology of Plants》2020,26(4):617-637
Physiology and Molecular Biology of Plants - The fruit is the most important economical organ in the grape; accordingly, to investigate the grapevine genomic methylation landscape and examine its... 相似文献