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181.
Liu Y Zhao R Shangguan D Zhang H Liu G 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2003,792(2):177-185
To replace conventional affinity ligand like protein A or protein G, a pseudobioaffinity ligand seems to be an alternative for the purification of immunoglobulin G (IgG). In this study, sulfamethazine (SMZ) was chosen as novel affinity ligand for investigating its affinity to human IgG. Monodisperse, non-porous, cross-linked poly (glycidyl methacrylate) (PGMA) beads were employed as the support for high-performance affinity chromatography. SMZ was immobilized on PGMA beads using bisoxirane (ethanediol diglycigyl ether) as spacer. The resultant affinity media presented minimal non-specific interaction with other proteins. Results of high-performance frontal analysis indicated that the media showed specific affinity to human IgG with a dissociation constant on the order of 10(-6) M. The SMZ affinity column proved useful for a very convenient one-step purification of IgG from human plasma. Antibody purity after a one-step purification was higher than 90%, as determined by densitometric scanning of sodium dodecyl sulfate-polyacrylamide gel electrophoresis of purified fraction under reducing condition. The results obtained indicate that SMZ is a valuable affinity ligand for purification of human IgG. 相似文献
182.
Shangguan Lingfei Fang Xiang Jia Haifeng Chen Mengxia Zhang Kekun Fang Jinggui 《Physiology and Molecular Biology of Plants》2020,26(4):617-637
Physiology and Molecular Biology of Plants - The fruit is the most important economical organ in the grape; accordingly, to investigate the grapevine genomic methylation landscape and examine its... 相似文献
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Tao Bing Tianjun Chang Cui Qi Nan Zhang Xiangjun Liu Dihua Shangguan 《Bioorganic & medicinal chemistry letters》2012,22(23):7052-7055
The screening of ligands against proteins plays important role in drug discovery and biological research. Using a dye labelled Streptavidin binding aptamer (SBA) as a competitive reporter probe, we found that adenosine bound to streptavidin specifically. Fluorescence spectral analysis showed that adenosine bound to both avidin and streptavidin with the Kds in the range of 0.1–0.2 mM, and these bindings can be blocked by biotin. Although streptavidin and avidin are well-known and widely used in bioanalysis, their biological role is still a riddle so far. Since adenosine is a ubiquitous physiological regulator present in cells, our finding provides new clues for the understanding of the functions of both proteins. 相似文献