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Farzaneh Moshtaghi Maleki Shahzad Iranipour Mir Jalil Hejazi Moosa Saber 《Archives Of Phytopathology And Plant Protection》2016,49(11-12):263-280
Grapevine moth, Lobesia botrana (Lep. Tortricidae) is a key pest of grape in Iran and other vineyards of the world. In this study, eight constant rearing temperatures (5, 10, 15, 20, 25, 30, 32 and 35 ± 1 °C) along with 60 ± 10% RH and a 16:8 (L:D) h photoperiod were chosen for demographic studies of the grapevine moth. Immature stages were unable to develop when reared at 5 and 35 °C, and the progeny moths were unable to successfully mate at 10, 15 and 32 °C. The overall developmental time of juveniles decreased at 30 °C (from 320.7 ± 3.4 d at 10 °C to 34.2 ± 0.2 d) followed by an increase to 42.5 ± 0.6 d at 32 °C. Based on values of the stable population growth parameters, the temperature of 25 °C was found to be optimal for propagation of grapevine moth. The highest values of the intrinsic rate of increase, gross and net reproductive rates were 0.0719 d??1, 55.5 and 23 females per generation, respectively, at 25 °C. Since jackknife and bootstrap estimates of mean and standard error were mainly similar, both methods may equally be used for uncertainty estimates. Our data suggest that cold storage of grapes will help to control grapevine moth infestations and damage. In many grape growing regions of Iran, the first generation is expected to cause damage. It is expected since our reproductive life table analysis suggests that the hot summer temperatures may restrict pest development during subsequent generations. 相似文献
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Ehsan Ullah Mughal Amina Sadiq Shahzad Murtaza Hummera Rafique Muhammad Naveed Zafar Tauqeer Riaz Bilal Ahmad Khan Abdul Hameed Khalid Mohammed Khan 《Bioorganic & medicinal chemistry》2017,25(1):100-106
The present study describes efficient and facile syntheses of varyingly substituted 3-thioaurones from the corresponding 3-oxoaurones using Lawesson’s reagent and phosphorous pentasulfide. In comparison, the latter methodology was proved more convenient, giving higher yields and required short and simple methodology. The structures of synthetic compounds were unambiguously elucidated by IR, MS and NMR spectroscopy. All synthetic compounds were screened for their inhibitory potential against in vitro acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. Molecular docking studies were also performed in order to examine their binding interactions with AChE and BChE human proteins. Both studies revealed that some of these compounds were found to be good inhibitors against AChE and BChE. 相似文献
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Ah-Young Kim Raheem Shahzad Sang-Mo Kang Abdul Latif Khan Seok-min Lee Yeon-Gyeong Park 《Journal of Plant Interactions》2017,12(1):244-253
This study aims to investigate the role of Paenibacillus terrae AY-38 to produce bioactive metabolites and to counteract pathogenic infections caused by B. cinerea. The pure culture of P. terrae (AY-38) showed the secretion of significant amount of IAA (indole-3-acetic acid) (109.57?±?3.2?µg?mL?1). The AY-38 strain also produced siderophore and glucanase, while in the in vitro test, it showed significant antagonism to Botrytis cinerea. In the in vivo plant experiment, the sole application of AY-38 significantly improved plant growth (plant height and leaf area), while in B. cinerea infected plants, AY-38 inoculation not only decreased the disease incidence on leaves and fruits but also reprogramed the plants for higher growth. AY-38 treatments promoted and rescued plant growth by modulating the defence responses of endogenous hormones, such as jasmonic and salicylic acids. Our findings concluded that P. terrae possesses great potential as a possible biocontrol agent against B. cinerea-induced pathogenic infections. 相似文献
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Sajjad Asaf Muhammad Aaqil Khan Abdul Latif Khan Muhammad Waqas Raheem Shahzad Ah-Yeong Kim 《Journal of Plant Interactions》2017,12(1):31-38
The objective of the present study was to determine the potential plant growth-promoting action of bacterial endophytes isolated from arid land-dwelling plants under normal conditions. Overall, five bacterial endophytes LK11 (Sphingomonas sp. LK11), TP5 (Bacillus subtilis), MPB5.3 (B. subtilis subsp. Subtilis), S9 (B. subtilis subsp. Subtilis), and TP1 (Serratia marcescens) were evaluated based on morphological characteristics after isolation and purification. Phytohormonal analysis of these endophytes predicted indole acetic acid (IAA) production 12.31?±?0.45?, 6.8?±?0.59, and 10.5?±?1.02?μM/mL in the culture broths of LK11, MPB5.3, and TP1, respectively. Under controlled greenhouse conditions, these endophytes were inoculated into soybean, and their growth-promoting characteristics were compared with those of non-phytohormone-producing endophytes. In terms of plant growth promotion, among IAA-producing endophytes, LK11 and TP1 greatly improved physiological characteristics such as shoot/root length, fresh/dry weight, and chlorophyll contents. However, the non-phytohormone-producing endophytes TP5 and S9 did not show a growth-promoting effect. Based on these results, plants inoculated with LK11 and TP1 along with a control were subjected to endogenous hormonal analysis and showed a significant increase in abscisic acid (457.30–398.55 vs. 205.93 ng/g D.W.) and a decrease in jasmonic acid content (50.07–85.07 vs. 93.90 ng/g D.W.), respectively. Total gibberellin content was found to significantly increase in endophyte-inoculated plants (155.43–146.94?ng/g D.W.) as compared to that in controls (113.76 ng/g D.W.). In summary, bacterial endophytes might be used to enhance crop plant physiological characteristics isolated from arid land-inhabiting plants under normal conditions. 相似文献
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Farooqi AA Bhatti S Nawaz A Khalid AM 《The International journal of biological markers》2011,26(2):75-81
The touchstone to evaluate accurately the aggressiveness and invasiveness of prostate cancer is something of a holy grail in the facet of urologic oncology. Gene expression and sequencing studies have improved our interpretations of the genetic determinants of the disease but are unsuccessful in the establishment of any unified classification to improve the molecular stratification. These questions addressing failure in rational drug design are difficult to answer in the multifaceted and heterogeneous pathogenesis of prostate cancer. In this review, we have developed a roadmap of the "recalcitrant prostate cancer proteome" to recognize the aspects of prostate cancer that may be helpful in effectively translating these findings to the clinic. 相似文献
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Rizwan Rasheed A. Wahid M. Farooq Iqbal Hussain Shahzad M. A. Basra 《Plant Growth Regulation》2011,65(1):35-45
High temperature strongly hampers the plant growth particularly at early growth stages. In this study, changes in some physiological
and anatomical characteristics and possibility of mitigating the adversities of heat stress by soaking sugarcane nodal buds
in 20 mM proline and glycinebetaine (GB) solutions have been explored. Heat stress reduced the rate of bud sprouting nonetheless
soaking the setts in proline followed by GB was beneficial. In addition, heat stress reduced the bud fresh and dry weights,
generated H2O2, reduced the tissue levels of K+ and Ca2+, while increased the osmolytes synthesis in a time course manner. Heat stress also delayed the emergence and expansion of
new bud leaves, by restricting the number and area of mesophyll cells. It also caused poor and aberrant development and diffused
appearance of mesophyll cells and vascular bundles in the bud leaves. However, soaking of buds in proline and GB solutions
substantially reduced the H2O2 production, improved the accumulation of soluble sugars and protected the developing tissues from heat stress effects; although
proline was more effective than GB. Correlations of various attributes indicated that soaking in GB and proline restricted
the H2O2 generation, improved K+ and Ca2+ contents, and increased the concentrations of free proline, GB and soluble sugars eventually improving the heat tolerance
of buds. Cost-benefit analysis showed that, considering increase in sprouting of buds, soaking in 20 mM solution of both osmoprotectants
is economical. 相似文献
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Rolfe MD Ter Beek A Graham AI Trotter EW Asif HM Sanguinetti G de Mattos JT Poole RK Green J 《The Journal of biological chemistry》2011,286(12):10147-10154
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The crystal structures of DsRed have shown that it contains an unusual non-proline containing cis peptide linkage. We have shown that it is also present in the precyclized immature form of DsRed, thereby eliminating the possibility that cis/trans isomerization drives the formation of the acylimine, which is responsible for DsRed's red fluorescence. Two mechanisms have been proposed for chromophore formation in green fluorescent protein (GFP), a "reduced" and an "oxidized" mechanism. DsRed adopts a tight turn conformation, such as that found in GFP, in the immature intermediate proposed in the oxidized mechanism, but not in the one predicted by the reduced mechanism. 相似文献