Organogenesis,direct somatic embryogenesis,and shoot proliferation of Rheum spiciforme Royle: an endemic and vulnerable medicinal herb from Indian Trans Himalayas

In Vitro Cellular & Developmental Biology - Plant - Rheum spiciforme Royle is a high value medicinal herb restricted to NW Himalayas. The medicinal properties of Rheum include anti-oxidant,...     

Photosynthetic Efficiency and Antioxidant Defense Potential are Key Players in Inducing Drought Tolerance in Transgenic Tobacco Plants Over-Expressing AVP1

Journal of Plant Growth Regulation - Plants have developed a number of physiological, biochemical, and molecular strategies to overcome water deficit conditions. Arabidopsis vacuolar...     

Synthesis of nonembryonic synseeds in Hemidesmus indicus R. Br.: short term conservation,evaluation of phytochemicals and genetic fidelity of the regenerants

Plant Cell, Tissue and Organ Culture (PCTOC) - Non-embryogenic, synthetic seeds were formed by encapsulating the nodal segments (NS) of Hemidesmus indicus R. Br. in calcium alginate hydrogel...     

Utilizing the Allelopathic Potential of Brassica Species for Sustainable Crop Production: A Review

Journal of Plant Growth Regulation - Sustainable crop production under changing climate is crucial to feed the increasing population of the world. Efforts are underway to discover novel strategies...     

Genetic association of insulin receptor substrate-1 (IRS-1, rs1801278) gene with insulin resistant of type 2 diabetes mellitus in a Pakistani population

Molecular Biology Reports - Insulin resistance (IR), a pathological condition of type 2 diabetes mellitus (T2DM) is characterized by an inability of body’s tissue to respond the secreted or...     

Plasma Diagnostic by Optical Emission Spectroscopy on Dolomite and Cross-Validation Using Scanning Electron Microscopy Coupled with Energy Dispersive X-ray Spectroscopy and Electron Probe Micro-Analysis

Plasma Physics Reports - Dolomite [CaMg(CO3)2] is an important carbonate mineral mainly composed of calcium magnesium carbonate. The correct determination of magnesium content in carbonate rocks is...     

In vitro propagation and assessment of genetic uniformity along with chemical characterization in Hildegardia populifolia (Roxb.) Schott & Endl.: a critically endangered medicinal tree

In Vitro Cellular & Developmental Biology - Plant - Hildegardia populifolia (Roxb.) Schott & Endl. is a critically endangered medicinal tree species which also provide high-quality...     

Comparative growth potential of thermophilic amylolytic Bacillus sp. on unconventional media food wastes and its industrial application

Amylases take part with vital role in industries such as food, fermentation; starch processing, textile and paper etc. Increasing amylases demand, high nutrient expenditure and environmental pollution have forced to utilize agro-industrial residues as a low-cost feedstock for enzyme production. In present study, three soil samples were collected from agro-industrial waste dumping areas in District Faisalabad. Ten thermophilic bacterial isolates were separated at 55 °C on the basis of colonial morphology, three isolates (F6, F11, F17) showed prominent zone of clearance applying iodine test on starch agar plates. Bacterial isolate F-11 showed highest amylase activity with DNS method and molecularly identified through 16S RNA sequencing as Bacillus sp. with Accession number MH917294. Four unconventional food wastes (banana, lemon, mango and potato) pretreated with 0.8% sulphuric acid concentrations taking 1000 g/L weight released the highest sugars contents and phenolic components. Maximum amylase activity i.e. 29.23 mg/ml was achieved in mango waste at, 40 °C, with pH 6.0 and 0.17% nitrogenous source adding 8% inoculum size (2 days old) using Response Surface Methodology (RSM) for optimization. Crude amylase confirmed its efficiency in starch hydrolysis that suggested it as potential candidate for application in starch industries.