Recently an association between a genetic variation in TRAF1/C5 and mortality from sepsis or cancer was found in rheumatoid arthritis (RA). The most prevalent cause of death, cardiovascular
disease, may have been missed in that study, since patients were enrolled at an advanced disease stage. Therefore, we used
an inception cohort of RA patients to investigate the association between TRAF1/C5 and cardiovascular mortality, and replicate the findings on all-cause mortality. As TRAF1/C5 associated mortality may not be restricted to RA, we also studied a large cohort of non-RA patients. 相似文献
High throughput screening led to the discovery of a novel series of 1,3-diaminopropan-2-ol sulfonamides as selective GlyT-1 inhibitors. Structure-activity relationships of this novel series and optimisation of the initial hit that led to the identification of (2), a potent and selective GlyT-1 inhibitor, are also presented. 相似文献
Myzus persicae is a devastating pest affecting potato production. Intron-containing hairpin RNA (ihpRNA) silenced the odorant-binding protein 8 (OBP8) for enhanced protection against Myzus persicae in potatoes. OBP8 was isolated from M. persicae, sequenced, with the allotment of GenBank ID. ERNAi was used to design siRNA targets from OBP8 with no off-targets. Multiple Sequence Alignment show M. persicae OBP8 resemblance with Acyrthosiphon pisum, Rhopalosiphum maidis, Aphis fabae, and Sitobion avenae. dsRNA-OBP8 (7 µg/µL) oral feeding resulted in a 69% mortality, and 58% OBP8 reduced expression 8D post-feeding compared to control. Golden Gate cloning is used for RNA interference taking advantage of type IIs restriction enzyme Eco31I. ihpRNA-OBP8 introduced by agroinfiltration in Solanum tuberosum. Transgenic S. tuberosum fed Myzus show 57.6% mortality and 49% reduction in OBP8 expression 8D post-feeding, compared to control. This work proves OBP8 as promising ihpRNA targets in potato and related crops for whom Myzus is a destructive pest.
Present study provides an easy and efficient protocol for large scale clonal propagation of Coleus forskohlii, a threatened medicinal plant of commercial importance. Basal leaf lamina excised from upper three nodes of shoot was used as explant and its size, position, orientation and season of collection were initially optimized to select the most responsive explant condition. Enhanced shoot production and proliferation has been achieved on medium containing 2 μM BA + 0.1 μM NAA wherein, a highest number of 35 shoots/explant were produced. The regenerated shoots of varied length (3–5 cm) were transferred to root induction medium comprising of IBA, NAA and IAA (1–5 μM) in half-strength MS medium to determine the most suitable shoot length for proper root induction. Rooted plantlets were acclimatized in field conditions after proper hardening. Histological analysis was also carried out to confirm the nature of origin of shoot buds from leaf explants. 相似文献
There have been many algorithms and software programs implemented for the inference of multiple sequence alignments of protein
and DNA sequences. The "true" alignment is usually unknown due to the incomplete knowledge of the evolutionary history of
the sequences, making it difficult to gauge the relative accuracy of the programs. 相似文献
The detection of relationships between a protein sequence of unknown function and a sequence whose function has been characterised
enables the transfer of functional annotation. However in many cases these relationships can not be identified easily from
direct comparison of the two sequences. Methods which compare sequence profiles have been shown to improve the detection of
these remote sequence relationships. However, the best method for building a profile of a known set of sequences has not been
established. Here we examine how the type of profile built affects its performance, both in detecting remote homologs and
in the resulting alignment accuracy. In particular, we consider whether it is better to model a protein superfamily using
a single structure-based alignment that is representative of all known cases of the superfamily, or to use multiple sequence-based
profiles each representing an individual member of the superfamily. 相似文献
Trichomonosis caused by Trichomonas vaginalis is the number one, non-viral sexually transmitted disease (STD) that affects more than 250 million people worldwide. Immunoglobulin
A (IgA) has been implicated in resistance to mucosal infections by pathogens. No reports are available of IgA-reactive proteins
and the role, if any, of this class of antibody in the control of this STD. The availability of an IgA monoclonal antibody
(mAb) immunoreactive to trichomonads by whole cell (WC)-ELISA prompted us to characterize the IgA-reactive protein of T. vaginalis. 相似文献
This research work was carried out to compare the color stripping efficiency of optimized biological method with the chemical stripping, commonly employed in the textile industries. Knitted fabric dyed with Reactive black B dye in 2, 4 and 6% shades strengths was subjected to chemical and biological stripping processes individually. Biological stripping process was found many fold superior to chemical one. It was noted that shade strength does not showed any pronounced effect on the bursting strength of fabric but biological and chemical treatment affect the quality of fabrics in terms of bursting strength/durability of fabric. White rot fungus Ganoderma lucidum IBL-05 showed good potential for decolorization/color stripping of cotton fabric dyed with Reactive black B under optimized set of conditions. The chemical stripping technology is inferior to biological stripping process regarding the quality of fabric and percent color removal from cotton fabric dyed with Reactive black B dye. 相似文献